Ki-67 and MCM-2 in Dental Follicle and Odontogenic Cysts: The Effects of Inflammation on Proliferative Markers

Abstract

The aim of this study was to investigate whether there is any association between inflammation and the expression of markers of cell cycle entry (Ki-67 and MCM-2) in dental follicle (DF) of asymptomatic impacted teeth and odontogenic cysts. The study consisted of 70 DFs and 20 odontogenic cysts (radicular cyst (RC), dentigerous cyst (DC) and keratocytic odontogenic tumor (KCOT) located at posterior mandibular region. Histological findings of inflammation for all specimen and mucous cell prosoplasia, squamous metaplasia, glandular epithelium for all DFs were stained with hematoxyline and eosin, periodic acid schiff, alcian blue, and mucin. Epithelial cell proliferation was determined by using immunohistochemical labeling for Ki-67 and MCM-2. The histologic examinations showed 16% mucous cell prosoplasia, 54% squamous metaplasia, 20% glandular epithelium, 37% inflammation. Inflammation was detected in all RCs and %62 in DF, %43 in DC and KCOT. Positive correlation was found between the inflammation of DF and odontogenic cysts (P < 0.01). The mean Ki-67 and MCM-2 expressions were found 9, 64 ± 5, 99 and 6, 34 ± 3, 81 in DF, 11, 85 ± 9, 01 and 13, 6 ± 9, 94 in odontogenic cysts, respectively. While the mean Ki-67 expressions were statistically significant in DF and KCOT (P < 0.01), MCM-2 were significant in RC and KCOT (P < 0.01). MCM-2 expresion in RCs were statistically significant than KCOT (P < 0.01). The results of this study indicated that the higher MCM-2 expressions in RC than the KCOT might be related to the inflammation and this protein might be more sensitive to inflammation.

Expression of podoplanin and TGF‐beta in glandular odontogenic cyst and its comparison with developmental and inflammatory odontogenic cystic lesions

“…Maxillary and mandibular odontogenic cysts are the most common cystic lesions in the human skeleton, which are further categorized as developmental and inflammatory . The lesions can originate from different cells like the remnants of odontogenic epithelium, epithelial cells of the oral mucosa and the dental follicle . Inflammatory odontogenic cysts result from inflammation, while the mechanism of the creation of developmental cysts is not clear .…”

Comparison of Proliferative Activity of Epithelial Cells between the Follicles of Impacted Teeth

“…The proliferative potential of dental follicles surrounding the crown of unerupted/impacted teeth has not yet been investigated in detail. Earlier studies focused on the follicles of impacted mandibular third molars (Szelachowska et al;Piattelli et al;Adelsperger et al, 2000;da Silva Baumgart et al, 2007;Oliveira et al, 2011Oliveira et al, , 2012Özarslan et al, 2013) and their associated pathologies (Saraçoglu et al, 2005;Edamatsu et al, 2005;Cabbar et al, 2008;Güler et al, 2012). To the knowledge of the authors, there is no data on the proliferative capacity of follicles of impacted teeth other than mandibular third molars.…”

“…Of the 48 specimens with epithelium lining, only one specimen in each group had reduced enamel epithelium, the rest were composed of stratified squamous epithelium. Some authors considered any dental follicle with squamous epithelium spreading along its surface as pathologic and the first visible sign of cystic transformation of the dental follicle (Oliveira et al, 2011;Cabbar et al;Güler et al;. According to Kim & Ellis (1993), evaluation of the dental follicles based solely on histological findings may lead to incorrect interpretation and the clinical, radiographic, and microscopic features should be considered in concert in arriving at a final diagnosis.…”

Study of the relationship between mononuclear inflammatory infiltrate and Ki‐67 and basement membrane and extracellular matrix protein expression in radicular cysts

“…In effect, the presence of proteolytic enzymes may cause the degradation of collagen in the basement membrane, leading to tissue destruction (Bilate , Güler et al . ).…”