KHDC1B Is a Novel CPEB Binding Partner Specifically Expressed in Mouse Oocytes and Early Embryos

Cai, Congli; Tamai, Keiko; Molyneaux, Kathleen

doi:10.1091/mbc.e10-03-0255

Cited by 13 publications

(17 citation statements)

References 50 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mammalian species have no comparable ortholog to maskin; the mammalian TACC3 gene codes for a protein similar to Xenopus maskin, but lacks the EIF4E binding domain (de Moor et al, ). Nevertheless, recent findings have pointed towards alternative models of translational repression that are maskin‐independent (Minshall et al, ; Cai et al, ; Tomek and Wollenhaupt ), and the expression pattern of CP‐associated genes observed herein is consistent with the activity of such post‐transcriptional regulatory mechanisms during bovine oocyte maturation.…”

Section: Discussionsupporting

confidence: 77%

RNA‐Seq profiling of single bovine oocyte transcript abundance and its modulation by cytoplasmic polyadenylation

Reyes

Chitwood

Ross

2015

Molecular Reproduction Devel

View full text Add to dashboard Cite

Molecular changes occurring during mammalian oocyte maturation are partly regulated by cytoplasmic polyadenylation (CP) and affect oocyte quality, yet the extent of CP activity during oocyte maturation remains unknown. Single bovine oocyte RNA sequencing (RNA-Seq) was performed to examine changes in transcript abundance during in vitro oocyte maturation in cattle. Polyadenylated RNA from individual germinal-vesicle and metaphase-II oocytes was amplified and processed for Illumina sequencing, producing approximately 30 million reads per replicate for each sample type. A total of 10,494 genes were found to be expressed, of which 2,455 were differentially expressed (adjusted P<0.05 and fold change >2) between stages, with 503 and 1,952 genes respectively increasing and decreasing in abundance. Differentially expressed genes with complete 3’-untranslated-region sequence (279 increasing and 918 decreasing in polyadenylated transcript abundance) were examined for the presence, position, and distribution of motifs mediating CP, revealing enrichment (85%) and lack there of (18%) in up- and down-regulated genes, respectively. Examination of total and polyadenylated RNA abundance by quantitative PCR validated these RNA-Seq findings. The observed increases in polyadenylated transcript abundance within the RNA-Seq data are likely due to CP, providing novel insight into targeted transcripts and resultant differential gene expression profiles that contribute to oocyte maturation.

show abstract

Section: Discussionsupporting

confidence: 77%

RNA‐Seq profiling of single bovine oocyte transcript abundance and its modulation by cytoplasmic polyadenylation

Reyes

Chitwood

Ross

2015

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…KHDC3L codes for a KH-domain containing protein, of which another member, KHDC1B, has been shown to bind mRNA and is believed to play a role in the regulation of their translation during oocyte maturation. 23 The closest NLRP gene to NLRP7 is NLRP2, which is located 25-kb distal to NLRP7 on human chromosome 19q13.4 in a head-to-head orientation. NLRP7 does not have rodent or bovine orthologues and is believed to have originated from a common NLRP2/7 ancestor in primates.…”

Section: Introductionmentioning

confidence: 99%

The genomic architecture of NLRP7 is Alu rich and predisposes to disease-associated large deletions

Reddy¹,

Nguyen²,

Sarrabay³

et al. 2016

Eur J Hum Genet

View full text Add to dashboard Cite

NLRP7 is a major gene responsible for recurrent hydatidiform moles. Here, we report 11 novel NLRP7 protein truncating variants, of which five deletions of more than 1-kb. We analyzed the transcriptional consequences of four variants. We demonstrate that one large homozygous deletion removes NLRP7 transcription start site and results in the complete absence of its transcripts in a patient in good health besides her reproductive problem. This observation strengthens existing data on the requirement of NLRP7 only for female reproduction. We show that two other variants affecting the splice acceptor of exon 6 lead to its in-frame skipping while another variant affecting the splice donor site of exon 9 leads to an in-frame insertion of 54 amino acids. Our characterization of the deletion breakpoints demonstrated that most of the breakpoints occurred within Alu repeats and the deletions were most likely mediated by microhomology events. Our data define a hotspot of Alu instability and deletions in intron 5 with six different breakpoints and rearrangements. Analysis of NLRP7 genomic sequences for repetitive elements demonstrated that Alu repeats represent 48% of its intronic sequences and these repeats seem to have been inserted into the common NLRP2/7 primate ancestor before its duplication into two genes.

show abstract

“…Maskin is a prime example of a species‐specific difference, as mammalian TACC3 lacks the eIF4E binding domain, suggesting an alternate protein or modified translational regulatory system may exist in mammals. Indeed, recent advances have revealed the potential for Maskin‐independent translational regulation . In mice, KH‐domain containing 1A ( Khdc1a ) and KH‐domain containing 1B ( Khdc1b ) were found to be expressed and translated in mouse oocytes, one of which is capable of binding eIF4e and CPEB .…”

Section: Mammalian Species Express Cp‐associated Factorsmentioning

confidence: 99%

“…40, 65 -69 In mice, KH-domain containing 1A (Khdc1a) and KH-domain containing 1B (Khdc1b) were found to be expressed and translated in mouse oocytes, one of which is capable of binding eIF4e and CPEB. 66 Alternatively, another protein containing a coiled-coil domain resembling TACC3 was discovered in cow oocytes, termed Maskin-like, and found not to bind CPEB but able to bind eIF4e preventing cap-binding. 65 Although KHDC1 and Maskin-like proteins are potential Maskin alternatives, further studies are required to elucidate their function in relation to gene-specific regulation and whether these proteins are conserved in other mammals.…”

Section: Figure 2 | Expression Of Polyadenylation and Translationalmentioning

confidence: 99%

Cytoplasmic polyadenylation in mammalian oocyte maturation

Reyes

Ross

2015

WIREs RNA

View full text Add to dashboard Cite

Oocyte developmental competence is the ability of the mature oocyte to be fertilized and subsequently drive early embryo development. Developmental competence is acquired by completion of oocyte maturation, a process that includes nuclear (meiotic) and cytoplasmic (molecular) changes. Given that maturing oocytes are transcriptionally quiescent (as are early embryos), they depend on post-transcriptional regulation of stored transcripts for protein synthesis, which is largely mediated by translational repression and deadenylation of transcripts within the cytoplasm, followed by recruitment of specific transcripts in a spatiotemporal manner for translation during oocyte maturation and early development. Motifs within the 3' untranslated region (UTR) of messenger RNA (mRNA) are thought to mediate repression and downstream activation by their association with binding partners that form dynamic protein complexes that elicit differing effects on translation depending on cell stage and interacting proteins. The cytoplasmic polyadenylation (CP) element, Pumilio binding element, and hexanucleotide polyadenylation signal are among the best understood motifs involved in CP, and translational regulation of stored transcripts as their binding partners have been relatively well-characterized. Knowledge of CP in mammalian oocytes is discussed as well as novel approaches that can be used to enhance our understanding of the functional and contributing features to transcript CP and translational regulation during mammalian oocyte maturation. WIREs RNA 2016, 7:71-89. doi: 10.1002/wrna.1316 For further resources related to this article, please visit the WIREs website.

show abstract

KHDC1B Is a Novel CPEB Binding Partner Specifically Expressed in Mouse Oocytes and Early Embryos

Cited by 13 publications

References 50 publications

RNA‐Seq profiling of single bovine oocyte transcript abundance and its modulation by cytoplasmic polyadenylation

RNA‐Seq profiling of single bovine oocyte transcript abundance and its modulation by cytoplasmic polyadenylation

The genomic architecture of NLRP7 is Alu rich and predisposes to disease-associated large deletions

Cytoplasmic polyadenylation in mammalian oocyte maturation

Contact Info

Product

Resources

About