KGC3P attenuates ovalbumin-induced airway inflammation through downregulation of p-PTEN in asthmatic mice

Lee, Young-sil; Yang, Won‐Kyung; Yee, Su-Min; Kim, Su-Man; Park, Yang-Chun; Shin, Han Jae; Han, Chang Kyun; Lee, Young Cheol; Kang, Hyung‐Sik; Kim, Seung-Hyung

doi:10.1016/j.phymed.2019.152942

Cited by 10 publications

(6 citation statements)

References 18 publications

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“…For example, M4a namely nepetin, is a natural flavonoid present in different plants. In recent years, accumulating evidence has shown that nepetin exhibits various pharmacological activities, especially potent anti-inflammatory properties, which might be related to the strong anti-inflammatory activity of eupatorin [30,31,32]. Overall, the identification of metabolites of eupatorin provides a basis for new pharmacological studies and these metabolites will be further explored in the future.…”

Section: Resultsmentioning

confidence: 99%

Identification of Metabolites of Eupatorin in Vivo and in Vitro Based on UHPLC-Q-TOF-MS/MS

Chen

Xue

et al. 2019

Molecules

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Eupatorin is the major bioactive component of Java tea (Orthosiphon stamineus), exhibiting strong anticancer and anti-inflammatory activities. However, no research on the metabolism of eupatorin has been reported to date. In the present study, ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) combined with an efficient online data acquisition and a multiple data processing method were developed for metabolite identification in vivo (rat plasma, bile, urine and feces) and in vitro (rat liver microsomes and intestinal flora). A total of 51 metabolites in vivo, 60 metabolites in vitro were structurally characterized. The loss of CH2, CH2O, O, CO, oxidation, methylation, glucuronidation, sulfate conjugation, N-acetylation, hydrogenation, ketone formation, glycine conjugation, glutamine conjugation and glucose conjugation were the main metabolic pathways of eupatorin. This was the first identification of metabolites of eupatorin in vivo and in vitro and it will provide reference and valuable evidence for further development of new pharmaceuticals and pharmacological mechanisms.

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Section: Resultsmentioning

confidence: 99%

Identification of Metabolites of Eupatorin in Vivo and in Vitro Based on UHPLC-Q-TOF-MS/MS

Chen

Xue

et al. 2019

Molecules

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“…A more popular animal model for airway inflammation is via the sensitization of ovalbumin (Y.-S. Lee, Yang, et al, 2019;Liang et al, 2017;J. Wang, Fu, et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…A more popular animal model for airway inflammation is via the sensitization of ovalbumin (Y.‐S. Lee, Yang, et al, 2019; Liang et al, 2017; J. Wang, Fu, et al, 2017). However, the use of ovalbumin as an allergen is less applicable to investigate pollution and PM‐induced airway inflammation.…”

Section: Introductionmentioning

confidence: 99%

Hypericum ascyron L. extract reduces particulate matter‐induced airway inflammation in mice

Lee

Yang

Han

et al. 2020

Phytotherapy Research

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The consequences of increased industrialization increased the risk of asthma and breathing difficulties due to increased particulate matter in the air. We aim to investigate the therapeutic properties of Hypericum ascyron L. extract (HAE) in airway inflammation and unravel its mechanism of action. We conducted nitric oxide and cell viability assay, real-time PCR and western blot analyses along with in vitro studies. in vivo studies include a model of coal fly ash and diesel exhaust particle (CFD)induced airway inflammation in mice. HAE reduced coal fly ash (CFA)-induced nitric oxide secretion without exhibiting cytotoxicity in MH-S cells. HAE also reduced the mRNA expression of pro-inflammatory cytokines and reduced the expression of proteins in the NFκB and MAPK pathways. In a mice model of CFD-induced airway inflammation, HAE effectively reduced neutrophil infiltration in bronchoalveolar lavage fluid (BALF) and increased the amount of T cells in the BALF, lungs, and blood while reducing all other immune cell subtypes to reduce airway inflammatory response. CXCL-1, IL-17, MIP-2, and TNF-α expression in the BALF were also reduced. HAE effectively reduced MIP-2 and TNF-α mRNA expression in the lung tissue of mice. In a nutshell, HAE is effective in preventing airway inflammation induced by CFA in MH-S cells, as well as inflammation induced by CFD in mice.

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“…In addition, α - and γ -mangostin, major xanthones present in mangosteen fruit ( Garcinia mangostana Linn), mitigate airway inflammation in OVA-induced allergic asthmatic mice by restoration of PTEN expression, which inactivates PI3K/Akt pathway and its downstream molecule NF- κ B, consequently reducing inflammatory cell recruitment into the airway and elevating the Th2 cytokine expression [ 135 ]. Lee's group reported that the Korean red ginseng extract and its component nepetin could attenuate Th2-derived cytokines, eosinophil infiltration, and airway remodeling in the OVA-induced allergic asthma model, and the mechanism is implicated in PPAR γ upregulation and PTEN-PI3K/Akt pathway inhibition by reducing the phosphorylation level of PTEN [ 136 ]. Although Korean ginseng extract and its component nepetin showed a promising antiasthmatic activity, no evidence for the active compounds and how to regulate PTEN phoshorylation was provided in this study.…”

Section: Pten In Chronic Lung Diseasesmentioning

confidence: 99%

PTEN: An Emerging Potential Target for Therapeutic Intervention in Respiratory Diseases

Cai

Yang

Jung

et al. 2022

Oxidative Medicine and Cellular Longevity

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Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a potent tumor suppressor that regulates several key cellular processes, including proliferation, survival, genomic integrity, migration, and invasion, via PI3K-dependent and independent mechanisms. A subtle decrease in PTEN levels or catalytic activity is implicated not only in cancer but also in a wide spectrum of other diseases, including various respiratory diseases. A systemic overview of the advances in the molecular and cellular mechanisms of PTEN involved in the initiation and progression of respiratory diseases may offer novel targets for the development of effective therapeutics for the treatment of respiratory diseases. In the present review, we highlight the novel findings emerging from current research on the role of PTEN expression and regulation in airway pathological conditions such as asthma/allergic airway inflammation, pulmonary hypertension (PAH), chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF), and other acute lung injuries (ALI). Moreover, we discuss the clinical implications of PTEN alteration and recently suggested therapeutic possibilities for restoration of PTEN expression and function in respiratory diseases.

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KGC3P attenuates ovalbumin-induced airway inflammation through downregulation of p-PTEN in asthmatic mice

Cited by 10 publications

References 18 publications

Identification of Metabolites of Eupatorin in Vivo and in Vitro Based on UHPLC-Q-TOF-MS/MS

Identification of Metabolites of Eupatorin in Vivo and in Vitro Based on UHPLC-Q-TOF-MS/MS

Hypericum ascyron L. extract reduces particulate matter‐induced airway inflammation in mice

PTEN: An Emerging Potential Target for Therapeutic Intervention in Respiratory Diseases

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