Key roles for the small leucine-rich proteoglycans in renal and pulmonary pathophysiology

Nastase, Madalina V.; Iozzo, Renato V.; Schaefer, Liliana

doi:10.1016/j.bbagen.2014.01.035

Cited by 57 publications

(57 citation statements)

References 192 publications

(269 reference statements)

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“…Furthermore, it is also of interest that the SLRPs may also regulate collagen turnover because several SLRPs bind and regulate the strong inducer of matrix formation, transforming growth factor-β, hereby possessing antifibrotic properties. 20,21 In contrast to the observed regulation of the collagen fibrilassociated proteoglycans, none of the abundant fibrillar collagens (I, III, and V), 22 which all were present in our data set, were regulated.…”

Section: Discussioncontrasting

confidence: 46%

Proteome Analysis of Human Arterial Tissue Discloses Associations Between the Vascular Content of Small Leucine-Rich Repeat Proteoglycans and Pulse Wave Velocity

Hansen

Beck

Irmukhamedov

et al. 2015

ATVB

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Objectives-We hypothesized that arterial stiffness is associated with changes in the arterial protein profile, particularly of extracellular matrix components. We aimed at determining differentially expressed proteins by quantitative proteome analysis in arterial tissue from patients with different degrees of arterial stiffness. Approach and Results-Arterial stiffness, assessed by carotid-femoral pulse wave velocity (PWV), central blood pressure and augmentation index by pulse wave analysis were measured the day before surgery in a group of patients undergoing coronary artery bypass grafting. Protein extracts of well-defined, homogenous, nonatherosclerotic individual samples of the left mammary artery from 10 of these patients with high PWV and 9 with low PWV were compared by quantitative proteome analysis, using tandem mass tag labeling and nano-liquid chromatography mass spectrometry/mass spectrometry. Of 418 quantified proteins, 28 were differentially expressed between the groups with high and low PWV (P<0.05). Three of 7 members of the extracellular matrix family of small leucine-rich repeat proteoglycans displayed significant differences between the 2 groups (P=0.0079; Fisher exact test). Three other ECM proteins were differentially regulated, that is, collagen, type VIII, α-1 and α-2 and collagen, type IV, α-1. Several proteins related to smooth muscle cell function and structure were also found in different amounts between the 2 groups. Conclusions-Changes in the arterial amounts of small leucine-rich proteoglycans, known to be involved in collagen fibrillogenesis, and of some nonfibrillar collagens in combination with alterations in proteins related to functions of the human arterial smooth muscle are associated with arterial stiffness, as determined by PWV. fibril organization and fibrillogenesis. 8 Mice knockout models for SLRPs display disorganized collagen fibril network and loses functions of the connective tissue and present with different disorders related to abnormal collagen fibril sizes and network, such as aortic dissection and rupture, skin fragility, joint laxity, and tendon weakness. 9,10 In this study, we hypothesized that patients with a high degree of arterial stiffness display a specific protein expression pattern different from patients with a normal low degree of arterial stiffness. We applied a quantitative proteomic approach to identify differentially expressed proteins in individual arterial tissue samples obtained from patients with high and low PWV. We investigate the internal mammary artery because this vessel has proven to be a suitable model artery for studies of generalized nonatherosclerotic arterial changes, that is, its matrix composition, endothelial function, and biochemistry reflect alterations in both the coronary and carotid arteries, as well as other features related to the arterial system. 11-13 MethodsMaterials and Methods are available in the online-only Data Supplement. ResultsBaseline patient characteristics are summarized in Table 1. There were no statistical d...

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Section: Discussioncontrasting

confidence: 46%

Proteome Analysis of Human Arterial Tissue Discloses Associations Between the Vascular Content of Small Leucine-Rich Repeat Proteoglycans and Pulse Wave Velocity

Hansen

Beck

Irmukhamedov

et al. 2015

ATVB

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“…Selected examples include kidney fibrosis,59 Marfan syndrome60 or epidermolysis bullosa 61. Decorin and other SLRPs are important in this context 62. Unlike in fibrotic diseases, TGFβ sequestration by decorin, as shown here, may have detrimental consequences in the development OA.…”

Section: Discussionmentioning

confidence: 81%

Forced exercise-induced osteoarthritis is attenuated in mice lacking the small leucine-rich proteoglycan decorin

et al. 2016

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“…Interestingly, instead of being a canonical DAMP, lumican, a keratan sulfate member of the SLRP family, acts as a helper of PAMP via binding to and presenting LPS to the TLR adaptor CD14 (19). For further details regarding the mechanism of SLRP release and their use as biomarkers in inflammation, please refer to recent more specific reviews (5,7,13,20,21).…”

Section: Damps Derived From the Extracellular Matrix (Ecm)mentioning

confidence: 99%

Complexity of Danger: The Diverse Nature of Damage-associated Molecular Patterns

Schaefer

2014

Journal of Biological Chemistry

Self Cite

519

467

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In reply to internal or external danger stimuli, the body orchestrates an inflammatory response. The endogenous triggers of this process are the damage-associated molecular patterns (DAMPs). DAMPs represent a heterogeneous group of molecules that draw their origin either from inside the various compartments of the cell or from the extracellular space. Following interaction with pattern recognition receptors in crosstalk with various non-immune receptors, DAMPs determine the downstream signaling outcome of septic and aseptic inflammatory responses. In this review, the diverse nature, structural characteristics, and signaling pathways elicited by DAMPs will be critically evaluated.

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Key roles for the small leucine-rich proteoglycans in renal and pulmonary pathophysiology

Cited by 57 publications

References 192 publications

Proteome Analysis of Human Arterial Tissue Discloses Associations Between the Vascular Content of Small Leucine-Rich Repeat Proteoglycans and Pulse Wave Velocity

Proteome Analysis of Human Arterial Tissue Discloses Associations Between the Vascular Content of Small Leucine-Rich Repeat Proteoglycans and Pulse Wave Velocity

Forced exercise-induced osteoarthritis is attenuated in mice lacking the small leucine-rich proteoglycan decorin

Complexity of Danger: The Diverse Nature of Damage-associated Molecular Patterns

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