Key Players in the Regulation of Fungal Secondary Metabolism

Knox, Benjamin P.; Keller, Nancy P.

doi:10.1007/978-1-4939-2531-5_2

Cited by 18 publications

(18 citation statements)

References 107 publications

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“…Such proteins, typically harboring a Zn(II) 2 Cys 6 TF domain, have been characterized in different fungal species (Knox and Keller, 2015). Nevertheless, the deletion of the genes encoding such regulators not always affects the expression of all the genes in the cluster.…”

Section: Botrydial Biosynthesis Depends On the Putative Pathway-specimentioning

confidence: 99%

“…In general, fungal SM gene clusters contain a gene encoding a Zn (II) 2 Cys 6 TF that specifically regulates the genes of the cluster (Brakhage, 2012;Knox and Keller, 2015). As the protein encoded by Bcbot6 displays this DNA-binding domain (IPR001138), we hypothesized that it might be a specific regulator of the BOT gene cluster even though the gene is separated from the Bcbot5 gene by a 10 kb-long A + T-rich stretch.…”

Section: Bcbot6 Regulates the Bcbot Genesmentioning

confidence: 99%

“…Around 60% of known fungal secondary metabolism gene clusters possess a pathway specific transcription factor (TF; Brakhage, 2012). In general, those proteins belong to the Zn(II) 2 -Cys 6 TF family and specifically regulate the genes of their corresponding cluster (Knox and Keller, 2015).…”

Section: Introductionmentioning

confidence: 99%

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The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6

Porquier¹,

Morgant²,

Moraga³

et al. 2016

Fungal Genetics and Biology

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a b s t r a c tBotrydial (BOT) is a non-host specific phytotoxin produced by the polyphagous phytopathogenic fungus Botrytis cinerea. The genomic region of the BOT biosynthetic gene cluster was investigated and revealed two additional genes named Bcbot6 and Bcbot7. Analysis revealed that the G + C/A + T-equilibrated regions that contain the Bcbot genes alternate with A + T-rich regions made of relics of transposable elements that have undergone repeat-induced point mutations (RIP). Furthermore, BcBot6, a Zn(II) 2 Cys 6 putative transcription factor was identified as a nuclear protein and the major positive regulator of BOT biosynthesis. In addition, the phenotype of the DBcbot6 mutant indicated that BcBot6 and therefore BOT are dispensable for the development, pathogenicity and response to abiotic stresses in the B. cinerea strain B05.10. Finally, our data revealed that B. pseudocinerea, that is also polyphagous and lives in sympatry with B. cinerea, lacks the ability to produce BOT. Identification of BcBot6 as the major regulator of BOT synthesis is the first step towards a comprehensive understanding of the complete regulation network of BOT synthesis and of its ecological role in the B. cinerea life cycle.

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Section: Botrydial Biosynthesis Depends On the Putative Pathway-specimentioning

confidence: 99%

Section: Bcbot6 Regulates the Bcbot Genesmentioning

confidence: 99%

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The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6

Porquier¹,

Morgant²,

Moraga³

et al. 2016

Fungal Genetics and Biology

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“…These motifs constitute an important group of regulatory elements in eukaryotes in which they act as ciselements (Knox & Keller 2015). Many transcription factors (TF) bind palindromic sequences with high affinity (Narlikar & Hartemink 2006;Qian et al 2006).…”

Section: Promoter Insertionsmentioning

confidence: 99%

“…The group of bZIP TFs target palindromic DNA sequences as dimers, thereby regulating e.g. secondary metabolism (Knox & Keller 2015). The importance of the palindromic sequences might explain the existence of isolates with few full repeats and a partial "A" element insertion in Pfcyp51 while they are categorized as DMI resistant (Figure 7 and S5).…”

Section: Promoter Insertionsmentioning

confidence: 99%

The origin, versatility and distribution of azole fungicide resistance in the banana black Sigatoka pathogen Pseudocercospora fijiensis

Aguirre

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A world‐wide analysis of reduced sensitivity to DMI fungicides in the banana pathogen Pseudocercospora fijiensis

Chong

Essoh

Isaza

et al. 2021

Pest Management Science

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BACKGROUND: Pseudocercospora fijiensis is the causal agent of the black leaf streak disease (BLSD) of banana. Bananas are important global export commodities and a major staple food. Their susceptibility to BLSD pushes disease management towards excessive fungicide use, largely relying on multisite inhibitors and sterol demethylation inhibitors (DMIs). These fungicides are ubiquitous in plant disease control, targeting the CYP51 enzyme. We examined sensitivity to DMIs in P. fijiensis field isolates collected from various major banana production zones in Colombia, Costa Rica, Dominican Republic, Ecuador, the Philippines, Guadalupe, Martinique and Cameroon and determined the underlying genetic reasons for the observed phenotypes. RESULTS:We observed a continuous range of sensitivity towards the DMI fungicides difenoconazole, epoxiconazole and propiconazole with clear cross-sensitivity. Sequence analyses of PfCYP51 in 266 isolates showed 28 independent amino acid substitutions, nine of which correlated with reduced sensitivity to DMIs. In addition to the mutations, we observed up to six

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Key Players in the Regulation of Fungal Secondary Metabolism

Cited by 18 publications

References 107 publications

The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6

The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6

The origin, versatility and distribution of azole fungicide resistance in the banana black Sigatoka pathogen Pseudocercospora fijiensis

A world‐wide analysis of reduced sensitivity to DMI fungicides in the banana pathogen Pseudocercospora fijiensis

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