Key Features of Common Lygus Species in the Central San Joaquin Valley

Mueller, Shannon; Goodell, Peter B.; Summers, Charles G.

doi:10.3733/ucanr.8105

Cited by 3 publications

(3 citation statements)

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“…Wild WTPBs were collected with entomological sweep nets at the alfalfa fields belonging to the Department of Animal Science at UC Davis, within an approx. 200 m radius from coordinates: N 38°32.038 0 , W 121°47.850 0 , elevation 34 ft. (10 m) WTPB individuals were sorted and a field key was used for their identification (Mueller et al 2003).…”

Section: Insect Supplymentioning

confidence: 99%

Polygalacturonase causes lygus-like damage on plants: cloning and identification of western tarnished plant bug (Lygus hesperus) polygalacturonases secreted during feeding

Celorio‐Mancera

Allen

Powell

et al. 2008

Arthropod-Plant Interactions

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Polygalacturonase (PG), an enzyme that degrades pectin within the plant tissue cell wall, has been postulated as the chemical cause of damage to plants by the mirid Lygus hesperus. Micro-injection of two pure recombinant Aspergillus niger PG II protein forms, the wild type enzymically active and the mutant inactive one, into alfalfa (Medicago sativa L.) florets, demonstrates that the enzymatic activity rather than the PG protein structure per se elicits damage symptoms. A PG gene family has been described for the tarnished plant bug, L. lineolaris. Here we report cloning members of the L. hesperus PG gene family, Lhpg2, obtained with L. lineolaris PG-specific primers and a novel Lhpg4, amplified with degenerate primers that were designed based, in part on the N-terminal sequence from an active, partially purified L. hesperus salivary gland PG protein. Proteomic analyses revealed that the salivary gland PGs encoded by Lhpg2 and Lhpg4 are detected in a diet into which L. hesperus has extruded its saliva when feeding.

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Section: Insect Supplymentioning

confidence: 99%

Polygalacturonase causes lygus-like damage on plants: cloning and identification of western tarnished plant bug (Lygus hesperus) polygalacturonases secreted during feeding

Celorio‐Mancera

Allen

Powell

et al. 2008

Arthropod-Plant Interactions

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“…200 m radius from coordinates: N 381 32.038', W 1211 47.850', elevation 34 ft. WTPB individuals were sorted and identified (Mueller et al, 2003).…”

Section: Insectsmentioning

confidence: 99%

Identification of endo‐ and exo‐polygalacturonase activity in Lygus hesperus (Knight) salivary glands

Celorio‐Mancera

Greve

Teuber

et al. 2008

Arch Insect Biochem Physiol

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Polygalacturonase (PG) activity found in the salivary gland apparatus of the western tarnished plant bug (WTPB, Lygus hesperus Knight) has been thought to be the main chemical cause of the damage inflicted by this mirid when feeding on its plant hosts. Early viscosity and thermal stability studies of the PG activity in L. hesperus protein extracts were difficult to interpret. Thus, it has been suggested that one or more PG protein(s) with different hydrolytic modes of action are produced by this mirid. In order to understand the quantitative complexity of the WTPB salivary PG activity, PG purification from a protein extract from salivary glands excised from L. hesperus insects was performed using affinity and ion exchange chromatography. To elucidate the qualitative complexity of the purified PGs, the digestion products generated by the PGs were separated using high performance anion exchange chromatography with pulsed amperometric detection. At least five PG proteins were detected; these differing in terms of their glycosylation, mass-to-charge ratios, and/or molecular mass. The characterization of the products generated by these PGs showed that endo- and exo-acting PGs are produced by WTPB. Although none of the PGs was purified to homogeneity, the present work provides biochemical evidence of a multiplicity of PGs that degrade the pectin component of the plant tissue in different fashions. The implications of these findings affect the understanding of WTPB feeding damage and, potentially, help identify ways to control this important crop pest. Arch. Insect Biochem. Physiol. 2008. (c) 2008 Wiley-Liss, Inc.

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“…The female has a groove that begins at the bottom and rises to the middle of her abdomen as the ovipositor lies in the center, almost hidden. This groove is absent for males and the abdomen is tapered at the end 26–28 . Cotton plants were sampled for L. lineolaris before and during the experiments.…”

Section: Methodsmentioning

confidence: 99%

Combining visual cues and pheromone blends for monitoring and management of the tarnished plant bug Lygus lineolaris (Hemiptera: Miridae)

George

Reddy

Little

et al. 2023

Pest Management Science

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BACKGROUND: The tarnished plant bug Lygus lineolaris (Palisot de Beauvois) is considered the most damaging pest of cotton (Gossypium hirsutum L.) in the mid-southern United States. Previous studies have reported the role of different ratios of volatile metathoracic gland components such as hexyl butyrate, (E)-2-hexenyl butyrate and (E)-4-oxo-2-hexenal in eliciting low-level attraction of L. lineolaris. In this study, we tested different visual cues (colored sticky cards) in combination with olfactory cues (pheromone blends) to optimize the attraction and capture of L. lineolaris in the field.RESULTS: Red-colored sticky cards were more attractive to L. lineolaris adults than white, blue or yellow cards. Red sticky cards combined with blends of three potential pheromone components attracted significantly more L. lineolaris adults than sticky cards without a blend added. Traps baited with a blend of hexyl butyrate, (E)-2-hexenyl butyrate and (E)-4-oxo-2-hexenal in 4:10:7 ratio, respectively, caught a significantly higher number of L. lineolaris than those baited with 10:4:2 or 7:10:4 blends or an unbaited control in the first week of the experiment. CONCLUSIONS: Combining visual cues (red color) with olfactory cues (pheromone blends) significantly increased the capture of L. lineolaris in the field. This device or a future iteration could contribute towards sustainable and environmentally appropriate early-season monitoring and management of L. lineolaris in the field.

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Key Features of Common Lygus Species in the Central San Joaquin Valley

Cited by 3 publications

References 0 publications

Polygalacturonase causes lygus-like damage on plants: cloning and identification of western tarnished plant bug (Lygus hesperus) polygalacturonases secreted during feeding

Polygalacturonase causes lygus-like damage on plants: cloning and identification of western tarnished plant bug (Lygus hesperus) polygalacturonases secreted during feeding

Identification of endo‐ and exo‐polygalacturonase activity in Lygus hesperus (Knight) salivary glands

Combining visual cues and pheromone blends for monitoring and management of the tarnished plant bug Lygus lineolaris (Hemiptera: Miridae)

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