Key Drivers Influencing the Large Scale Production of Xylitol

Hou-rui, Zhang

doi:10.1007/978-3-642-31887-0_12

Cited by 11 publications

(9 citation statements)

References 36 publications

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“…Biotechnological production of xylitol from lignocellulosic biomass has been widely studied as an alternative to the commercial chemical process, since neither purification of xylose nor high temperatures and pressures are required for this biologically selective process. 11 , 12 , 13 This bioprocess is based on the ability of pentose-assimilating yeasts to reduce xylose to xylitol as the first step of xylose metabolism, with participation of NAD(P)H-dependent xylose reductase (XR, E.C.1.1.1.21). 14 , 15 , 16 According to these authors, accumulation of xylitol is caused by a NADH/NAD + imbalance, i.e., low levels of NAD + and high levels of NADH, resulting from limited O 2 supply and a consequent reduction in cell respiratory rate.…”

Section: Introductionmentioning

confidence: 99%

Sugarcane straw as a feedstock for xylitol production by Candida guilliermondii FTI 20037

Hernández-Pérez

Arruda

Felipe

2016

Brazilian Journal of Microbiology

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Sugarcane straw has become an available lignocellulosic biomass since the progressive introduction of the non-burning harvest in Brazil. Besides keeping this biomass in the field, it can be used as a feedstock in thermochemical or biochemical conversion processes. This makes feasible its incorporation in a biorefinery, whose economic profitability could be supported by integrated production of low-value biofuels and high-value chemicals, e.g., xylitol, which has important industrial and clinical applications. Herein, biotechnological production of xylitol is presented as a possible route for the valorization of sugarcane straw and its incorporation in a biorefinery. Nutritional supplementation of the sugarcane straw hemicellulosic hydrolyzate as a function of initial oxygen availability was studied in batch fermentation of Candida guilliermondii FTI 20037. The nutritional supplementation conditions evaluated were: no supplementation; supplementation with (NH4)2SO4, and full supplementation with (NH4)2SO4, rice bran extract and CaCl2·2H2O. Experiments were performed at pH 5.5, 30 °C, 200 rpm, for 48 h in 125 mL Erlenmeyer flasks containing either 25 or 50 mL of medium in order to vary initial oxygen availability. Without supplementation, complete consumption of glucose and partial consumption of xylose were observed. In this condition the maximum xylitol yield (0.67 g g−1) was obtained under reduced initial oxygen availability. Nutritional supplementation increased xylose consumption and xylitol production by up to 200% and 240%, respectively. The maximum xylitol volumetric productivity (0.34 g L−1 h−1) was reached at full supplementation and increased initial oxygen availability. The results demonstrated a combined effect of nutritional supplementation and initial oxygen availability on xylitol production from sugarcane straw hemicellulosic hydrolyzate.

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Section: Introductionmentioning

confidence: 99%

Sugarcane straw as a feedstock for xylitol production by Candida guilliermondii FTI 20037

Hernández-Pérez

Arruda

Felipe

2016

Brazilian Journal of Microbiology

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“…The xylose solution is hydrogenated to xylitol at high pressure (4-7 MPa) and moderate temperatures (80-140 • C) with hydrogen gas and a metal catalysis e.g., Raney-Nickel (Rafiqul and Sakinah, 2013;Gerbrandt, 2014;Delgado Arcaño et al, 2018). The xylitol solution is crystallized and the mother liquor containing the remaining contaminants is separated from the xylitol crystals, which are dried before packaging (Hou-Rui, 2012;Rafiqul and Sakinah, 2013).…”

Section: Process/product Options Considered For the Valorization Of W...mentioning

confidence: 99%

“…Bioprocesses involving enzymatic fermentation can be used to produce xylitol, although the technology is to our knowledge only developed at the pilot scale (Rafiqul and Sakinah, 2013;zuChem, 2013). The bioprocess does not require a purified xylose solution because the sugar conversion to xylitol is more selective (Hou-Rui, 2012). After the PHL post-hydrolysis, the hydrolyzate is detoxified to remove inhibitors such as phenolic compounds, furfural, HMF, organic acids, and non-organic components (Mpabanga et al, 2012).…”

Section: Process/product Options Considered For the Valorization Of W...mentioning

confidence: 99%

Comparing Biorefinery Processes at the Early Design Stage Using Large Block Analysis

2022

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The transformation of pulp and paper mills through the integration of biorefineries is increasingly considered essential to the future of many existing sites. However, evaluating the risk and return of different biorefinery process alternatives at the early design stage is challenging. There are many strategies and technologies that must be considered, each of which is typically accompanied by its unique risks, including high levels of uncertainty in capital and operating cost estimates often obtained from technology providers. The novel methodology presented in this study, called Large Block Analysis (LBA), comprises a systematic approach for addressing these important challenges at the early design stage. LBA is used to obtain relative cost estimates for six process/product combinations incorporating different Technology Readiness Levels (TRLs), for adding value to a hemicellulose stream extracted from hardwood chips. In this case study, it was found that the fixed capital costs obtained using the LBA method differed from the original costs by between 121 and −19%, and operating cost estimates differed by between 117 and −17% from the original. The results show that the most economically-viable options for the hemicellulose stream having reasonable technology risk included the production of (1) animal feed additives, (2) xylitol using a variant of the classical chemical process, and (3) furfural.

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“…Xylitol production in crude hemicellulosic hydrolysates using S. cerevisiae has been reported using birch wood, corn, and wheat as natural xylose sources (Delgado Arcaño et al, 2020; Hou-Rui et al, 2012; Jofre et al, 2022). At the same time, Candida spp.…”

Section: Introductionmentioning

confidence: 99%

Strategies for improved xylitol production in batch fermentation of sugarcane hydrolysate using Saccharomyces cerevisiae

Lizarazo¹,

Pereira²,

Mello³

2022

Preprint

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A plethora of studies have focused on improvements of xylitol production. The challenges of establishing a biotechnological route for the industrial production of this sugar have been explored using different microorganisms and renewable feedstock. Nevertheless, sugarcane biomass has been neglected as the pentose source for xylitol production using Saccharomyces cerevisiae. Therefore, here we investigate the use of an industrial S. cerevisiae strain for xylitol production in batch fermentation of non-detoxified sugarcane straw hydrolysate, envisioning the diversification of the current infrastructure used for second-generation bioethanol production from the same lignocellulosic material. In order to optimize the xylose conversion in a non-fed cultivation system, guidelines in cell inoculum and medium supplementation are suggested, as well as the first attempt to use electro-fermentation for this purpose. Accordingly, our results show that the increase in initial cell density and hydrolysate supplementation allows a xylitol production of 19.24 ± 0.68 g/L, representing 0,132 g/L.h productivity.

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Key Drivers Influencing the Large Scale Production of Xylitol

Cited by 11 publications

References 36 publications

Sugarcane straw as a feedstock for xylitol production by Candida guilliermondii FTI 20037

Sugarcane straw as a feedstock for xylitol production by Candida guilliermondii FTI 20037

Comparing Biorefinery Processes at the Early Design Stage Using Large Block Analysis

Strategies for improved xylitol production in batch fermentation of sugarcane hydrolysate using Saccharomyces cerevisiae

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