Key Apoptotic Pathways for Heat-Induced Programmed Germ Cell Death in the Testis

Hikim, Amiya P. Sinha; Lue, Yanhe; Yamamoto, Cindy M.; Vera, Yanira; Rodriguez, Susana; Yen, Pauline H.; Soeng, Kevin; Wang, Christina; Swerdloff, Ronald S.

doi:10.1210/en.2003-0175

Cited by 179 publications

(109 citation statements)

References 51 publications

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“…Apoptosis is a normal process for mammal germ cells during spermatogenesis. In adult rats, these losses are mostly observed during spermatogonial development and to a lesser extend during maturation of spermatocytes and spermatids (Hikim et al 2003). Apoptotic appearances of spermatids are rare because apoptotic cells are phagocytosed by sustentacular cells (Blanco-Rodriguez and Martinez-Garcia 1999;Cai et al 1997;Hikim and Swerdloff 1999).…”

Section: Discussionmentioning

confidence: 99%

Effects of Hexachlorocyclohexane (HCH-γ-Isomer, Lindane) Intoxication on the Proliferation and Apoptosis in Rat Testes

et al. 2009

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In this study, experimentally lindane-induced histopathological changes and proliferation and/or apoptosis in germ cells in the rat testes were investigated. A total of 40 healthy fertile 3-month-old male rats were used. Animals were divided into 4 groups, each containing 10 rats. Group 1 (control) was given only pure olive oil, Groups 2, 3 and 4 were administered lindane at 10, 20 and 40 mg/kg/bw, respectively, by gastric gavage for 30 days. Microscopically, degenerative changes were observed in the lindane-treated groups. For proliferative activity PCNA immunolabelling and for germ cells apoptosis TUNEL methods were performed. Although a strong PCNA positivity in the control group was observed, a gradual decrease was noted in the lindane-treated groups especially at higher doses. Significant increases of apoptosis were seen in the lindane-treated groups compared to the control group. A decrease in testosterone concentrations was observed in lindane-treated groups compared to the control group. The study indicates that high-dose lindane intoxication contributes to the suppression of spermatogenesis through a reduction of germ cell proliferation and an increase of germ cell death in rat testes.

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Section: Discussionmentioning

confidence: 99%

Effects of Hexachlorocyclohexane (HCH-γ-Isomer, Lindane) Intoxication on the Proliferation and Apoptosis in Rat Testes

et al. 2009

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“…Immunohistochemistry: Caspase Detection in Testicular Tissue Immunohistochemistry was used in several studies to investigate caspases in testicular tissue (Giampietri et al, 2005;Hikim et al, 2003;Kim et al, 2007;Pentikainen et al, 1999;Tesarik et al, 2002;Zheng et al, 2006).…”

Section: Colorimetric Assaysmentioning

confidence: 99%

Impact of caspase activation in human spermatozoa

Grunewald

Sharma

Paasch

et al. 2009

Microscopy Res & Technique

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Caspases are central components in the apoptosis signaling cascade. The family of cysteine proteases transduces and enhances the apoptosis signal, and activation of effector caspases results in controlled cellular degradation. Although initially the presence of caspases in spermatozoa was controversially discussed in recent years, many studies demonstrated their activation in male germ cells. Activated apoptosis signaling results in decreased fertilizing capacity of the sperm. This review presents the current knowledge on the role of caspases in human sperm. Techniques of caspase monitoring are highlighted. With regard to the high impact of caspases on the sperm fertilizing potential, physiological and pathological settings of caspase activation and inactivation are discussed. Finally, the effects of depletion of caspase-positive sperm are shown with various standard and molecular sperm preparation methods. Microsc. Res. Tech. 00:000-000, 2009. V

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“…The presence of programmed cell death or apoptosis in somatic as well as germ cells, especially during spermatogenesis, is well established (Hikim et al 2003;Shaha 2007;Jia et al 2009). However, the presence of apoptotic markers in ejaculated spermatozoa is contentious (Martin et al 2004;Brum et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway

Rahman

Vandaele²,

Rijsselaere

et al. 2014

Reprod. Fertil. Dev.

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Abstract. Heat stress has long been recognised as a cause of subfertility in farm animals. The objectives of the present study were to elucidate the effect of heat stress on sperm function and involvement of the mitogen-activated protein kinase (MAPK) 14 signalling pathway. Spermatozoa incubated for 4 h at a physiological temperature (38.58C) exhibited significantly (P , 0.05) reduced motility, plasma membrane integrity and mitochondrial potential compared with nonincubated spermatozoa; the reductions in these parameters were more severe following incubation at a hyperthermic (418C) temperature (P , 0.01). Percentages of fertilisation and embryo development were highly affected in spermatozoa incubated at 418C compared with non-incubated spermatozoa (P , 0.01). Similarly, embryo quality was adversely affected by sperm incubation at 418C, as indicated by a higher apoptotic cell ratio in Day 7 blastocysts compared with that in the non-incubated control group (14.6% vs 6.7%, respectively; P , 0.01). Using SB203580 (10 mg mL À1 ), a specific inhibitor of the p38 MAPK pathway, during sperm hyperthermia reduced MAPK14 activation (24.9% vs 35.6%), increased sperm motility (45.8% vs 26.5%) and reduced DNA fragmentation (16.9% vs 23.4%) compared with the untreated control group, but did not improve subsequent fertilisation and embryo development. In conclusion, heat stress significantly affects the potential of spermatozoa to penetrate oocytes, as well as subsequent embryo development and quality. Notably, the data show that the MAPK14 signalling pathway is largely involved in heat-induced sperm damage. However, further research is needed to elucidate other signalling pathways possibly involved in heat-induced sperm damage.Additional keywords: functional parameters, mitogen-activated protein kinase 14 phosphorylation, terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling.

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Key Apoptotic Pathways for Heat-Induced Programmed Germ Cell Death in the Testis

Cited by 179 publications

References 51 publications

Effects of Hexachlorocyclohexane (HCH-γ-Isomer, Lindane) Intoxication on the Proliferation and Apoptosis in Rat Testes

Effects of Hexachlorocyclohexane (HCH-γ-Isomer, Lindane) Intoxication on the Proliferation and Apoptosis in Rat Testes

Impact of caspase activation in human spermatozoa

Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway

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