Ketoisomeric conversion of glucose derived from microalgal biomasses

Lee, Da Gyung; Choi, Doo Jin; Park, Jae Kweon

doi:10.1016/j.procbio.2015.03.011

Cited by 8 publications

(6 citation statements)

References 43 publications

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“…However, until 1976, sucrose was the main sweetener derived from sugar beet (40%) and sugar cane (60%). In recent years, attention has also been placed on defatted microalgal biomasses as substrates for producing fructose with immobilized GI, resulting in various monosaccharides, such as glucose, galactose, fructose, and xylose. − …”

Section: Biocatalytic Processesmentioning

confidence: 99%

Glucose Isomerization by Enzymes and Chemo-catalysts: Status and Current Advances

et al. 2017

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The well-known interconversion of aldoses to their corresponding ketoses was discovered more than a century ago, but has recently attracted renewed attention due to alternative application areas. Since the pioneering discovery, much work has been directed toward improving the process of isomerization of aldoses in terms of yields, catalysts, solvents, catalytic systems, etc., by both enzymatic and chemo-catalytic approaches. Among aldose–ketose interconversion reactions, fructose production by glucose isomerization to make high-fructose corn syrup (HFCS) is an industrially important and large biocatalytic process today, and a large number of studies have been reported on the process development. In parallel, also alternative chemo-catalytic systems have emerged, as enzymatic conversion has drawbacks, though they are typically more selective and produce fructose under mild reaction conditions. Isomerization of glucose is also a central reaction for making renewable platform chemicals, such as lactic acid, 5-hydroxymethylfurfural (HMF), and levulinic acid. In these other applications, thermally stable catalysts are required, thus making use of enzymatic catalysis inadequate, since enzymes generally possess a limited temperature operating window, typically less than 80 °C. From this viewpoint, the chemo-catalystsespecially solid heterogeneous catalystsare playing a key role for the development of not only making HFCS, but also making chemicals and fuels from glucose via the isomerized product/intermediate fructose. This review focuses on how both enzyme- and chemo-catalysts are being useful for the isomerization of glucose to fructose. Specifically, development of Lewis acid-containing zeolites for glucose isomerization is reviewed in detail, including mechanism, isotopic labeling, and computational studies.

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Section: Biocatalytic Processesmentioning

confidence: 99%

Glucose Isomerization by Enzymes and Chemo-catalysts: Status and Current Advances

et al. 2017

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“…El-Shora et al [24] found their glucose isomerase from Bacillus thuringiensis isomerizing best at pH of 7.0. Lee et al [32] reported pH of 6.0 as optimum for Dunaliellater tiolecta. Mu et al [33] observed optimal pH at 6.5 for Acidothermus cellulolyticus.…”

Section: Achromobacter Xylosoxidans Mck-4 Started Accumulating Intracellular Enzyme After 12 Hrsmentioning

confidence: 98%

“…El-Shora et al [24] found out that glucose isomerase from Bacillus thuringiensis isomerizing best at 50°C. Lee et al [32] observed the optimal activity of GI from Dunaliellater tiolecta to be 60°C. Mu et al [33] found optimal isomerization temperature of 80 ˚C for GI from Acidothermus cellulolyticus.…”

Section: Achromobacter Xylosoxidans Mck-4 Started Accumulating Intracellular Enzyme After 12 Hrsmentioning

confidence: 99%

Production, Characterization and Optimal Performance Studies of Glucose Isomerase by Achromobacter xylosoxidans mck-4 Isolated from Starch Milling Wastes

Mbagwu¹,

Egong²,

Akan³

2018

MRJI

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Seventy-five (75) bacterial isolates from starch milling wastes in Nsukka, Nigeria were screened for their ability to produce glucose isomerase (GI). The isolate Achromobacter xylosoxidans Mck-4 gave the highest activity of intracellular GI using starch casein agar (SCA) medium supplemented with nystatin to eliminate fungi contaminations, incubated at 30°C for 48 hours and pH 7. The SDS PAGE showed that the enzyme was partially purified and the band was near the protein marker of 43 KDa. The enzyme was purified 4.7 fold with a specific activity of 6.4±0.52 U/mg of protein and Original Research Article

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“…The production of fructose from lactose includes two steps, i.e., first, lactose hydrolysis into glucose and galactose using β-galactosidase and, second, glucose isomerisation to fructose by glucose isomerase (GI) [9]. Fructose is usually used as a sweet substance in food and beverage production, as well as an additive for special food for diabetics [10]. It is sweeter than sucrose and can be absorbed more slowly than glucose, as well as being easily metabolised without the involvement of insulin [11].…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of Lactose into Glucose–Galactose Syrup by Two-Stage Enzymatic Hydrolysis

Majore

Ciproviča

2022

Foods

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Fermentation technology enables the better use of resources and the conversion of dairy waste into valuable food products. The aim of this study is to evaluate the conversion rate of glucose into fructose by immobilised glucose isomerase (GI) in sweet and acid whey permeates for glucose–galactose syrup production. The experiments demonstrated that the highest concentration of glucose and galacto-oligosaccharides (GOSs) in sweet and acid whey permeates was reached by GODO-YNL2 β-galactosidase, 32 ± 2% and 28 ± 1%, respectively. After glucose isomerisation, the highest fructose yield was 23 ± 0.3% and 13 ± 0.4% in sweet and acid whey permeates, where Ha-Lactase 5200 β-galactosidase was used for lactose hydrolysis in sweet and acid whey permeates. Finally, the results of this study highlight the potential for two-stage enzymatic hydrolysis to increase the sweetness of glucose–galactose syrup made from sweet and acid whey permeates.

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Ketoisomeric conversion of glucose derived from microalgal biomasses

Cited by 8 publications

References 43 publications

Glucose Isomerization by Enzymes and Chemo-catalysts: Status and Current Advances

Glucose Isomerization by Enzymes and Chemo-catalysts: Status and Current Advances

Production, Characterization and Optimal Performance Studies of Glucose Isomerase by Achromobacter xylosoxidans mck-4 Isolated from Starch Milling Wastes

Bioconversion of Lactose into Glucose–Galactose Syrup by Two-Stage Enzymatic Hydrolysis

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