Keratose/poly (vinyl alcohol) blended nanofibers: Fabrication and biocompatibility assessment

Ju, Wang; Hao, Shilei; Luo, Tao; Zhou, Tao; Yang, Xin; Wang, Bochu

doi:10.1016/j.msec.2016.11.071

Cited by 42 publications

(29 citation statements)

References 28 publications

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“…55 kDa). Moreover, SDS-PAGE analysis indicated that the recombinant proteins were more purified than the keratin extracts, since the only bands could be observed in the gel [11,20]. Inclusion body proteins instead of soluble proteins were obtained in this study, which could be explained by the fact that human hair keratins are not water-soluble in nature.…”

Section: Molecular Weight Of the K37 And K81mentioning

confidence: 70%

“…Human hair keratins are highly cross-linked proteins typically containing a-helix and are rich in cystine, threonine, serine and proline [10]. The common extraction methods, including oxidative reaction, reductive reactions and enzyme hydrolysis, are used to break the cystine bonds of insoluble proteins [6,11,12]. These extraction procedures hardly control the amino acid composition and molecular weight (MW) of extracted keratins due to the random break of cystine bonds [10].…”

Section: Introductionmentioning

confidence: 99%

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Recombinant human hair keratin proteins for halting bleeding

Guo

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Keratins derived from human hair have been widely used for tissue engineering. However, some drawbacks relative to the traditional keratins extracts have been found: (a): difficultly controlling the amino acid composition; (b): batch to batch inconsistent quality; and (c): producing complex keratin and keratin-associated proteins (KAPs), which problems have made some studies concerning human hair keratins stagnant, especially in the mechanism studies related to hemostasis of keratins. Herein, a type-I human hair keratin of K37 and a type-II human hair keratin of K81 were heterologously expressed and firstly used for haemostatic application. SDS-PAGE analysis shows that the recombinant keratins had higher purity compared to the extracted keratins. The circular dichroism (CD) spectra of K37 and K81 suggested that the secondary structures were rich in α-helix. In addition, the recombinant keratin proteins could enhance fibrin colt formation at the site of injury and decrease the bleeding time and blood loss in liver puncture and femoral artery injury rat models. This study provides a new strategy for future works involving design and mechanism studies of keratin biomaterials.

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Section: Molecular Weight Of the K37 And K81mentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Recombinant human hair keratin proteins for halting bleeding

Guo

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…At the same time, keratin markedly increases the fiber hydrophilicity compared with pure PCL, which improved the adhesion and proliferation of human mesenchymal stem cells [127]. Similarly, co-electrospinning of keratose (i.e., oxidative keratin) with PVA resulted in nanofibers with uniform fibrous structure, suitable hydrophilicity and mechanical properties [125]. Properties of electrospun keratin nanofibers were also improved by incorporation of hydrotalcites, intended for delivery of diclofenac.…”

Section: Nature-derived Nanofibers Degradable In the Human Tissuesmentioning

confidence: 98%

“…Keratin is a fibrous structural protein, present in skin appendages, such as hair, wool, feather, nails, horns, claws, hooves, and in the outer (cornified) layer of epidermis [125,126]. Keratin protects epithelial cells from damage and stress and is insoluble in water and organic solvents.…”

Section: Nature-derived Nanofibers Degradable In the Human Tissuesmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Bačáková¹,

Pajorová²,

Zikmundová³

et al. 2020

Current and Future Aspects of Nanomedicine

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Nanofibrous scaffolds belong to the most suitable materials for tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. This chapter is focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is the largest and vitally important organ in the human body. Nanofibrous meshes can serve as substrates for adhesion, growth and differentiation of skin and stem cells, and also as an antimicrobial and moistureretaining barrier. These meshes have been prepared from a wide range of synthetic and nature-derived polymers. This chapter is focused on the use of nature-derived polymers. These polymers have good or limited degradability in the human tissues, which depends on their origin and on the presence of appropriate enzymes in the human tissues. Non-degradable and less-degradable polymers are usually produced in bacteria, fungi, algae, plants or insects, and include, for example, cellulose, dextran, pullulan, alginate, pectin and silk fibroin. Well-degradable polymers are usually components of the extracellular matrix in the human body or at least in other verte brates, and include collagen, elastin, keratin and hyaluronic acid, although some polymers produced by non-vertebrate organisms, such as chitosan or poly(3-hydroxybutyrate-co-3-hydroxyvalerate), are also degradable in the human body.Current and Future Aspects of Nanomedicine 2 microbial infection, and at the same time, they can keep appropriate moisture and gas exchange at the wound site.Nanofibrous scaffolds for skin tissue engineering have been fabricated from a wide range of synthetic and nature-derived polymers, which can be either biostable or degradable within the human body. Biostable synthetic polymers used in nanofiber-based skin regenerative therapies include, for example, polyurethane [2], polydimethylsiloxane [3], polyethylene terephthalate [4], polyethersulfone [5], and also hydrogels such as poly(acrylic acid) (PAA, [6]), poly(methyl methacrylate) (PMMA, [7]), and poly[di(ethylene glycol) methyl ether methacrylate] (PDEGMA, [8]). Degradable synthetic polymers typically include poly(ε-caprolactone) (PCL, [9]) and its copolymers with polylactides (PLCL, [10]), polylactides (PLA, [11]) and their copolymers with polyglycolides (PLGA, [12]), and also so-called auxiliary polymers, such as poly(ethylene glycol) (PEG), poly(ethylene oxide) (PEO, [13]) or poly(vinyl alcohol) (PVA, [14]), which facilitated the electrospinning process and improved the mechanical properties and wettability of the chief polymer. However, the synthetic polymers, although they are well-chemically defined and tailorable, are often bioinert, hydrophobic and thus not promoting cell adhesion, and also not well-adhering to the wound site. Therefore, they need to be combined with other bioactive substances, particularly nature-derived polymers.This chapter is focused on nature-derived polymers used for fabrication of nanofibrous scaffolds for skin tissue engineering and wound healing. The advantages of...

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“…Since keratin is not thermoplastic, the blending must be carried out using a common suitable solvent [16] [17]. For instance, water was previously used to process keratin/poly(ethylene oxide) [18] and keratin/polyvinyl alcohol [19] while formic acid was used for keratin/silk-fibroin [20], keratin/polyamide 6 [21] and keratin/poly-(caprolacton) blends [22]. In particular, polyamide 6 is a synthetic polymer having a molecular structure similar to proteins and it has been successfully exploited to process keratin-based solutions into films and nanofibres mats that were tested for adsorption of heavy metal ions in waters and VOCs like formaldehyde in air [8] [21].…”

Section: Introductionmentioning

confidence: 99%