Keratose hydrogel for tissue regeneration and drug delivery

Ledford, Benjamin T.; Barron, Catherine; Dyke, Mark Van; He, Jia‐Qiang

doi:10.1016/j.semcdb.2021.06.017

Cited by 8 publications

(7 citation statements)

References 73 publications

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“…The accuracy of this analysis is testified by the low number of chemicals that are considered as misclassified (3/358). These results would seem to confirm that keratin can safely be used for human applications as already tested for keratins in other forms. ,,,, Although in silico tests are very accurate, in vitro and in vivo tests still need to confirm these results.…”

Section: Resultsmentioning

confidence: 68%

“…The keratin must be extracted by wool, and this extraction can be performed using techniques such as reduction, sulfitolysis, alkali hydrolysis, enzymatic hydrolysis, ionic liquid treatment, microwave irradiation, steam explosion, and oxidation. − The oxidation method of extraction allows one to obtain the soluble fraction of keratin, keratoses, breaking the disulfide bonds and converting some thiol in cysteic acids . Typically, keratoses are used alone or in blends with other polymers for making fibers, films, sponges, and hydrogels for the biomedical field. , More specifically, many reports demonstrate keratin’s potential, mainly in the form of hydrogels for drug delivery, wound healing, nerve regeneration, dental implants, and vascular smooth muscle differentiation . In addition, keratin can bind metals and its properties can be tailored, resulting in biomaterials with different mechanical, swelling, and chelating properties.…”

Section: Introductionmentioning

confidence: 99%

“… 41 , 42 More specifically, many reports demonstrate keratin’s potential, mainly in the form of hydrogels for drug delivery, wound healing, nerve regeneration, dental implants, and vascular smooth muscle differentiation. 43 In addition, keratin can bind metals and its properties can be tailored, resulting in biomaterials with different mechanical, swelling, and chelating properties.…”

Section: Introductionmentioning

confidence: 99%

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Keratose Self-Cross-Linked Wound Dressing for Iron Sequestration in Chronic Wounds

Anceschi,

Patrucco,

Bhavsar

et al. 2023

ACS Omega

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Chronic wound diseases affect a large part of the world population, and therefore, novel treatments are becoming fundamental. People with chronic wounds show high iron and protease levels due to genetic disorders or other comorbidities. Since it was demonstrated that iron plays an important role in chronic wounds, being responsible for oxidative processes (ROS generation), while metalloproteinases prevent wound healing by literally "eating" the growing skin, it is crucial to design an appropriate wound dressing. In this paper, a novel bioactive dressing for binding iron in chronic wounds has been produced. Wool-derived keratose wound dressing in the form of films has been prepared by casting an aqueous solution of keratoses. These films are water-soluble; therefore, in order to increase their stability, they have been made insoluble through a thermal cross-link treatment. Fourier transform infrared (FTIR), differential scanning calorimetry (DSC), and thermogravimetric analyzer (TGA) analyses clarified the structure and the properties of the keratose wound dressing films. The capability of this new biomaterial in iron sequestration has been investigated by testing the adsorption of Fe 3+ by inductively coupled plasma-optical emission spectrometry (ICP-OES). The results suggest that the keratose cross-linked films can adsorb a large amount of iron (about 85% of the average amount usually present in chronic wounds) following pseudo-second-order kinetics and an intraparticle diffusion model, thus opening new perspectives in chronic wound care. Furthermore, the QSAR Toolbox was applied for conducting in silico tests and for predicting the chemical behavior of the C-Ker-film. All of the data suggest that the keratose bioactive dressing can significantly contribute to wound healing by mechanisms such as iron depletion, acting as a radical scavenger, diminishing the proteolytic damage, acting as a substrate in place of skin, and, finally, promoting tissue regeneration.

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Section: Resultsmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Keratose Self-Cross-Linked Wound Dressing for Iron Sequestration in Chronic Wounds

Anceschi,

Patrucco,

Bhavsar

et al. 2023

ACS Omega

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“…It is noteworthy that the keratin protein KRT family, S100A protein family, and SPRR genes (small proline‐rich protein) exhibit significant downregulation in certain subpopulations of both LNM (−) OTSCC tissue and LNM (+) OTSCC tissue. These genes have been previously associated with tumor migration, infiltration, and metastasis 47–49 . In this study, a specific group of epithelial tumor cells was identified as having elevated levels of SPRR3, which is a cross‐linked envelope protein found in keratinocytes.…”

Section: Discussionmentioning

confidence: 87%

“…These genes have been previously associated with tumor migration, infiltration, and metastasis. [47][48][49] In this study, a specific group of epithelial tumor cells was identified as having elevated levels of SPRR3, which is a crosslinked envelope protein found in keratinocytes. SPRR3 has been identified as a prognostic biomarker for lung adenocarcinoma and has been observed to be overexpressed in human OTSCC stem-like cells.…”

Section: Discussionmentioning

confidence: 99%

Single‐cell RNA sequencing reveals the heterogeneity of epithelial cell and fibroblast cells from non‐ to metastatic lymph node OTSCC

Song,

Yang,

Sun

et al. 2024

The FASEB Journal

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Lymph node metastasis (LNM) is one of the common features of oral tongue squamous cell carcinoma (OTSCC). LNM is also taken as a sign of advanced OTSCC and poor survival rate. Recently, single‐cell RNA sequencing has been applied in investigating the heterogeneity of tumor microenvironment and discovering the potential biomarkers for helping the diagnosis and prognosticating. Pathogenesis of LNM in OTSCC remains unknown. Specifically, cancer‐associated fibroblasts (CAFs) and epithelial tumor cells could foster the progression of tumors. Thus, in this study, we aimed to comprehensively analyze the roles of subpopulations of CAFs and epithelial tumor cells in lymph node metastatic OTSCC using the integration of OTSCC single‐cell RNA sequencing datasets. Four distinct subtypes of CAFs, namely vascular CAFs, myofibroblast CAFs, inflammatory CAFs, and growth arrest CAFs were successfully discovered in LNM tumor and confirmed the roles of GAS and PTN pathways in the progression of tumor metastasis. In addition, NKAIN2+ epithelial cells and FN1+ epithelial cells specifically exhibited an upregulation of PTN, NRG, MIF, and SPP1 signaling pathways in the metastatic OTSCC. In doing so, we put forth some potential biomarkers that could be utilized for the purpose of diagnosing and prognosticating OTSCC during its metastatic phase and tried to confirm by immunofluorescence assays.

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Optimization of Keratin Sponge Preparation Conditions for Hemostatic Application Using Response Surface Methodology (RSM)

Goudarzi,

Dadashian,

Vatanara

et al. 2023

J Polym Environ

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Keratose hydrogel for tissue regeneration and drug delivery

Cited by 8 publications

References 73 publications

Keratose Self-Cross-Linked Wound Dressing for Iron Sequestration in Chronic Wounds

Keratose Self-Cross-Linked Wound Dressing for Iron Sequestration in Chronic Wounds

Single‐cell RNA sequencing reveals the heterogeneity of epithelial cell and fibroblast cells from non‐ to metastatic lymph node OTSCC

Optimization of Keratin Sponge Preparation Conditions for Hemostatic Application Using Response Surface Methodology (RSM)

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