Keratoconus: I. Biochemical studies

Critchfield, James W.; Calandra, Anthony J.; Nesburn, Anthony B.; Kenney, M. Cristina

doi:10.1016/s0014-4835(88)80047-2

Cited by 66 publications

(36 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The literature describing collagen levels in keratoconic corneae is inconclusive: Critch®eld et al (1988) described decreased collagen and total protein levels in keratoconic tissue by western blotting. Radda et al (1982) found a 5 % increase in type I collagen in keratoconus, while Zimmermann et al (1988) found no differences in collagen composition of biochemical extracts from keratoconus.…”

Section: Extracellular Matrix Proteinsmentioning

confidence: 99%

Cellular Incursion into Bowman's Membrane in the Peripheral Cone of the Keratoconic Cornea

Sherwin

Brookes

Loh

et al. 2002

Experimental Eye Research

View full text Add to dashboard Cite

Section: Extracellular Matrix Proteinsmentioning

confidence: 99%

Cellular Incursion into Bowman's Membrane in the Peripheral Cone of the Keratoconic Cornea

Sherwin

Brookes

Loh

et al. 2002

Experimental Eye Research

View full text Add to dashboard Cite

“…[34][35][36][37][38] MMPs are inhibited by tissue inhibitors of MMP (TIMPs) which comprise a family of four protease inhibitors, TIMP-1, -2, -3, and -4. 39 Thus, MMPs are widely assumed to have a central role in the pathogenesis of keratoconus.…”

mentioning

confidence: 99%

Resistance of Corneal RFUVA–Cross-Linked Collagens and Small Leucine-Rich Proteoglycans to Degradation by Matrix Metalloproteinases

Zhang¹,

Mao²,

Schwend³

et al. 2013

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

PURPOSE. Extracellular matrix metalloproteinases (MMPs) are thought to play a crucial role in corneal degradation associated with the pathological progression of keratoconus. Currently, corneal cross-linking by riboflavin and ultraviolet A (RFUVA) has received significant attention for treatment of keratoconus. However, the extent to which MMPs digest cross-linked collagen and small leucine-rich proteoglycans (SLRPs) remains unknown. In this study, the resistance of RFUVA-cross-linked collagens and SLRPs to MMPs has been investigated. METHODS.To investigate the ability of MMPs to digest crosslinked collagen and SLRPs, a model reaction system using purified collagen type I, type IV, and nonglycosylated, commercially available recombinant SLRPs, keratocan, lumican, mimecan, decorin, and biglycan in solution in vitro has been compared using reactions inside an intact bovine cornea, ex vivo.RESULTS. Our data demonstrate that corneal cross-linked collagen type I and type IV are resistant to cleavage by MMP-1, MMP-2, MMP-9, and MMP-13, whereas non-cross-linked collagen I, IV, and natively glycosylated SLRPs are susceptible to degradation by MMPs. In addition, both cross-linked SLRPs themselves and cross-linked polymers of SLRPs and collagen appear able to resist degradation. These results suggest that the interactions between SLRPs and collagen caused by RFUVA protect both SLRPs and collagen fibrils from cleavage by MMPs. CONCLUSIONS.A novel approach for understanding the biochemical mechanism whereby RFUVA cross-linking stops keratoconus progression has been achieved. (Invest Ophthalmol Vis Sci.

show abstract

“…1 Histopathologic studies of keratoconic corneas 2,3 show decreased numbers of collagen fibrils with normal morphology, keratocyte abnormalities, basement membrane fragmentation, iron deposition in the basal epithelial layer, disintegration of Bowman membrane, and breaks in Descemet membrane. Biochemical 4,5 studies show a decrease in total protein and abnormalities in expression levels of degradative enzymes and protease inhibitors, leading to decreased mechanical and biochemical stability of the cornea.…”

mentioning

confidence: 99%

Imaging corneal crosslinking by autofluorescence 2-photon microscopy, second harmonic generation, and fluorescence lifetime measurements

Steven

Hovakimyan

Guthoff

et al. 2010

Journal of Cataract and Refractive Surgery

View full text Add to dashboard Cite

show abstract

Keratoconus: I. Biochemical studies

Cited by 66 publications

References 12 publications

Cellular Incursion into Bowman's Membrane in the Peripheral Cone of the Keratoconic Cornea

Cellular Incursion into Bowman's Membrane in the Peripheral Cone of the Keratoconic Cornea

Resistance of Corneal RFUVA–Cross-Linked Collagens and Small Leucine-Rich Proteoglycans to Degradation by Matrix Metalloproteinases

Imaging corneal crosslinking by autofluorescence 2-photon microscopy, second harmonic generation, and fluorescence lifetime measurements

Contact Info

Product

Resources

About