Keratoblast and Keratocyte, not Keratinocyte

Hamilton, A. Ian

doi:10.1038/238098a0

Cited by 9 publications

(2 citation statements)

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“…Newly assembled immature collagen fibrils assemble, grow, and mature during postnatal development of the cornea (Birk et al, 1996;Birk, 2001). The corneal keratocyte is the major cell type in the corneal stroma that synthesizes the stromal extracellular matrix (Hamilton, 1972;Beales et al, 1999;Fini, 1999;Chakravarti et al, 2004). It secretes two fibril-forming collagens.…”

Section: Introductionmentioning

confidence: 99%

Collagen fibril assembly during postnatal development and dysfunctional regulation in the lumican‐deficient murine cornea

Chakravarti

Zhang

Chervoneva

et al. 2006

Developmental Dynamics

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The transparent cornea is the outer barrier of the eye and is its major refractive surface. Development of a functional cornea requires a postnatal maturation phase involving development, growth and organization of the stromal extracellular matrix. Lumican, a leucine-rich proteoglycan, is implicated in regulating assembly of collagen fibrils and the highly organized extracellular matrix essential for corneal transparency. We investigated the regulatory role(s) of lumican in fibril assembly during postnatal corneal development using wild type (Lum ϩ/ϩ ) and lumican-null (Lum Ϫ/Ϫ ) mice. In Lum ϩ/ϩ mice, a regular architecture of small-diameter fibrils is achieved in the anterior stroma by postnatal day 10 (P10), while the posterior stroma takes longer to reach this developmental maturity. Thus, the anterior and the posterior stroma follow distinct developmental timelines and may be under different regulatory mechanisms. In Lum Ϫ/Ϫ mice, it is the posterior stroma where abnormal lateral associations of fibrils and thicker fibrils with irregular contours are evident as early as P10. In contrast, the anterior stroma is minimally perturbed by the absence of lumican. In Lum ϩ/ϩ mice, lumican is expressed throughout the developing stroma at P10, with strong expression limited to the posterior stroma in the adult. Therefore, the posterior stroma, which is most vulnerable to lumican-deficiency, demonstrates an early developmental defect in fibril structure and architecture in the Lum Ϫ/Ϫ mouse. These defects underlie the reported increased light scattering and opacity detectable in the adult. Our findings emphasize the early regulation of collagen structure by lumican during postnatal development of the cornea. Developmental Dynamics 235:2493-2506, 2006.

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Section: Introductionmentioning

confidence: 99%

Collagen fibril assembly during postnatal development and dysfunctional regulation in the lumican‐deficient murine cornea

Chakravarti

Zhang

Chervoneva

et al. 2006

Developmental Dynamics

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“…A study had showed high functional and tissue specific activity with the use of three 5 truncated fragments of the keratin K12 promoter in human corneal epithelial cell lines [195]. The stroma of the cornea consists of many cell types, most of the cells being keratocytes [196]. Keratocan is a cornea stroma-specific keratan sulfate proteoglycan (KSPG) expressed in adult vertebrate cornea [195].…”

Section: Promoter Selection For Targeted Therapeuticsmentioning

confidence: 99%

Corneal Regeneration Using Gene Therapy Approaches

Sarkar

Panikker

D’Souza

et al. 2023

Cells

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One of the most remarkable advancements in medical treatments of corneal diseases in recent decades has been corneal transplantation. However, corneal transplants, including lamellar strategies, have their own set of challenges, such as graft rejection, delayed graft failure, shortage of donor corneas, repeated treatments, and post-surgical complications. Corneal defects and diseases are one of the leading causes of blindness globally; therefore, there is a need for gene-based interventions that may mitigate some of these challenges and help reduce the burden of blindness. Corneas being immune-advantaged, uniquely avascular, and transparent is ideal for gene therapy approaches. Well-established corneal surgical techniques as well as their ease of accessibility for examination and manipulation makes corneas suitable for in vivo and ex vivo gene therapy. In this review, we focus on the most recent advances in the area of corneal regeneration using gene therapy and on the strategies involved in the development of such therapies. We also discuss the challenges and potential of gene therapy for the treatment of corneal diseases. Additionally, we discuss the translational aspects of gene therapy, including different types of vectors, particularly focusing on recombinant AAV that may help advance targeted therapeutics for corneal defects and diseases.

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Regional variations in mouse skin : Quantitation of epidermal compartments in two different body sites

Christophers

Laurence

1972

Virchows Arch. Abt. B Zellpath.

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Keratoblast and Keratocyte, not Keratinocyte

Cited by 9 publications

References 0 publications

Collagen fibril assembly during postnatal development and dysfunctional regulation in the lumican‐deficient murine cornea

Collagen fibril assembly during postnatal development and dysfunctional regulation in the lumican‐deficient murine cornea

Corneal Regeneration Using Gene Therapy Approaches

Regional variations in mouse skin : Quantitation of epidermal compartments in two different body sites

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