Keratinocytes derived from embryonic stem cells induce wound healing in mice

Uluer, Elgin Türköz; Vatansever, Hafize Seda; Aydede, Hasan; Özbilgin, Mahmut Kemal

doi:10.1080/10520295.2018.1541479

Cited by 9 publications

(9 citation statements)

References 48 publications

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“…These results indicate that poriferan chitin may be potentially useful in skin tissue regeneration. Fibroblasts and keratinocytes are two major groups of cells that take part in wound healing [ 42 , 67 , 74 ]. This process requires several phases: hemostasis, formation of a fibrin clot, inflammation/granulation, re-epithelialization, and tissue remodeling.…”

Section: Resultsmentioning

confidence: 99%

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

et al. 2021

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Tissue engineering (TE) is a field of regenerative medicine that has been experiencing a special boom in recent years. Among various materials used as components of 3D scaffolds, naturally formed chitinous materials seem to be especially attractive because of their abundance, non-toxic and eco-friendly character. In this study, chitinous skeleton isolated from the marine sponge Aplysina fistularis (phylum: Porifera) was used for the first time as a support for the cultivation of murine fibroblasts (Balb/3T3), human dermal fibroblasts (NHDF), human keratinocyte (HaCaT), and human neuronal (SH-SY5Y) cells. Characterization techniques such as ATR FTIR, TGA, and μCT, clearly indicate that an interconnected macro-porous, thermostable, pure α-chitin scaffold was obtained after alkali–acid treatment of air-dried marine sponge. The biocompatibility of the naturally formed chitin scaffolds was confirmed by cell attachment and proliferation determined by various microscopic methods (e.g., SEM, TEM, digital microscopy) and specific staining. Our observations show that fibroblasts and keratinocytes form clusters on scaffolds that resemble a skin structure, including the occurrence of desmosomes in keratinocyte cells. The results obtained here suggest that the chitinous scaffold from the marine sponge A. fistularis is a promising biomaterial for future research about tissues regeneration.

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Section: Resultsmentioning

confidence: 99%

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

et al. 2021

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“…EGF plays a significant role during intestinal development in establishing a selective intestinal barrier (reviewed in 24 ). The source of circulating EGF could be epithelial cells and/or stem cells of the skin, the respiratory, or intestinal epithelium 25–29 . EGF receptor (EGFR) signaling regulates various processes, including tight junction protein expression, autophagy, and apoptosis of epithelial cells, and further modulates the bacterial and fungal colonization of the epithelium 30–32 …”

Section: Discussionmentioning

confidence: 99%

“…The source of circulating EGF could be epithelial cells and/or stem cells of the skin, the respiratory, or intestinal epithelium. [25][26][27][28][29] EGF receptor (EGFR) signaling regulates various processes, including tight junction protein expression, autophagy, and apoptosis of epithelial cells, and further modulates the bacterial and fungal colonization of the epithelium. [30][31][32] When we analyzed the relation between EGF and IgE sensitization to allergens, we observed that early-life EGF levels were higher in those children who did not develop allergen-specific IgE…”

Section: Discussionmentioning

confidence: 99%

Higher circulating EGF levels associate with a decreased risk of IgE sensitization in young children

Reinert-Hartwall

Siljander

Härkönen

et al. 2021

Pediatric Allergy Immunology

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Background Decreased exposure to microbial agents in industrialized countries and urban living areas is considered as a risk factor of developing immune‐mediated diseases, such as allergies and asthma. Epithelial surfaces in the gastrointestinal and respiratory tracts and in the skin constitute the primary areas in contact with the environmental microbial load. Methods We analyzed the levels of 30 cytokines and growth factors in serum or plasma as markers of the immune maturation in the participants in the DIABIMMUNE study from Russian Karelia (n = 60), Estonia (n = 83) and Finland (n = 89), three neighboring countries with remarkable differences in the incidences of allergies, asthma and autoimmune diseases. Results We observed an upregulation of T helper cell signature cytokines during the first 12 months of life, reflecting natural development of adaptive immune responses. During the first years of life, circulating concentrations of epidermal growth factor (EGF) were significantly higher, especially in Russian children compared with Finnish children. The children who developed IgE sensitization showed lower levels of EGF than those without such responses. Conclusion Our results suggest that low circulating EGF levels associate with the risk of allergies possibly via the effects on the epithelial integrity and mucosal homeostasis.

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“…mesoderm, de nitive endoderm and ectoderm, which in turn give rise to progenitor and mature cells of various lineages. Differentiation of mESC to generate epithelial cells for the purpose of tissue engineering and wound healing has been reported (33,34), but it has not been used to explore the genetic and/or environmental factors as the underlying etiology of epithelial disorders.…”

Section: Introductionmentioning

confidence: 99%

The combined effects of Map3k1 mutation and dioxin on differentiation of keratinocytes derived from mouse embryonic stem cells

Wang

Xiao

Kimura

et al. 2022

Preprint

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Epithelial development starts with stem cell commitment to ectoderm followed by differentiation to basal keratinocytes. The basal keratinocytes, first committed in embryogenesis, constitute the basal layer of the epidermis. They have robust proliferation and differentiation potential, giving rise to suprabasal cells, and are responsible for expansion, maintenance and regeneration of the epidermis. We generated basal epithelial cells in vitro through differentiation of mouse embryonic stem cells (mESCs). Early on in differentiation, the expression of stem cell markers, Oct4 and Nanog, decreased rapidly along with increased ectoderm marker keratin (Krt) 18. Later on, Krt 18 expression was subdued when cells displayed basal keratinocyte characteristics, including regular polygonal shape, adherent and tight junctions and Krt 14 expression. Using Map3k1 mutant mESCs and environmental dioxin, we examined the gene and environment effects on differentiation. Neither Map3k1 mutation nor dioxin altered mESC differentiation to ectoderm and basal keratinocytes, but they, individually and in combination, potentiated Krt 1 expression and basal to spinous differentiation. Similar gene-environment effects were observed in vivo where dioxin exposure increased Krt 1 more substantially in the epithelium of Map3k1+/− than wild type embryos. Thus, the in vitro model of epithelial differentiation can detect genetic and environmental effects on epidermal development.

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Keratinocytes derived from embryonic stem cells induce wound healing in mice

Cited by 9 publications

References 48 publications

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

Higher circulating EGF levels associate with a decreased risk of IgE sensitization in young children

The combined effects of Map3k1 mutation and dioxin on differentiation of keratinocytes derived from mouse embryonic stem cells

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