2007
DOI: 10.1152/ajplung.00460.2006
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Keratinocyte growth factor protects against elastase-induced pulmonary emphysema in mice

Abstract: Pulmonary emphysema is characterized by persistent inflammation and progressive alveolar destruction. The keratinocyte growth factor (KGF) favorably influences alveolar maintenance and repair and possesses anti-inflammatory properties. We aimed to determine whether exogenous KGF prevented or corrected elastase-induced pulmonary emphysema in vivo. Treatment with 5 mg x kg(-1) x day(-1) KGF before elastase instillation prevented pulmonary emphysema. This effect was associated with 1) a sharp reduction in broncho… Show more

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Cited by 55 publications
(56 citation statements)
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“…11 In some experiments, iNOS À/À and eNOS À/À animals were used, with wild-type C57BL/6 mice as controls (Jackson Laboratory, Bar Harbor, ME, USA). In other experiments, wild-type mice (Janvier, Le Genest Saint-Isle, France) were treated subcutaneously with two injections of 10 mg/kg (20 mg/kg/ day) N-3-aminomethyl-benzyl-acetamidine-dihydrochloride (1400W, Cayman Chemical, Ann Arbor, MI, USA), a specific iNOS inhibitor, 12 from the day before tracheal instillation to the day they were killed, whereas control mice received saline injections.…”
Section: Materials and Methods Animal Modelmentioning
confidence: 99%
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“…11 In some experiments, iNOS À/À and eNOS À/À animals were used, with wild-type C57BL/6 mice as controls (Jackson Laboratory, Bar Harbor, ME, USA). In other experiments, wild-type mice (Janvier, Le Genest Saint-Isle, France) were treated subcutaneously with two injections of 10 mg/kg (20 mg/kg/ day) N-3-aminomethyl-benzyl-acetamidine-dihydrochloride (1400W, Cayman Chemical, Ann Arbor, MI, USA), a specific iNOS inhibitor, 12 from the day before tracheal instillation to the day they were killed, whereas control mice received saline injections.…”
Section: Materials and Methods Animal Modelmentioning
confidence: 99%
“…The sequences of primers are indicated in Supplementary Table 1. Normalization was achieved by quantification of the expression of either CXXC1 or ribosomal protein 13 mRNA (RPL13) as described previously, 11 chosen as control housekeeping genes (HKG) among four HKG tested because of their stable expression in the different conditions of the experiment (KO iNOS and 1400W-treated mice with or without tracheal instillation of elastase), and/or their adequate expression level as compared with the considered target gene. All PCR conditions were adjusted in order to obtain equivalent optimal amplification efficiency between the different assays.…”
Section: Determination Of Nnos Inos and Enos Mrna And Protein Levelmentioning
confidence: 99%
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“…The trachea was surgically exposed, and 5 U in 50 mL of porcine pancreatic elastase (purchased from Elastin Products, Owensville, MO) was injected intratracheally in nine mice (elastase-instilled group), whereas the rest of the animals (N ¼ 5) were instilled with 50 mL of saline (control group; Ref. 15). All animals survived the instillation protocol.…”
Section: Emphysema Experimental Modelmentioning
confidence: 99%
“…antichemokine or receptor blockade in hyperoxia-exposed rats [17,65] pentoxyfilline in VLBW infants [64] No adverse effects observed inhibitory peptide in mouse model [43] curcumin [66], simvastatin [67], lovastatin [68], or HO-1 [69] Retinoids RA in hyperoxic model in rats [82] RA in angiogenic inhibition in mice [83] vitamin A in premature baboons [86] RA in rats and mice with or without inhibited alveolar septation [77,81] RA in mouse models [85] Growth factor supplementation VEGF [54] or FGF7 [98] in hyperoxia-exposed rats HGF [99] or EPO [83] in mouse models VEGF injurious in mouse prenatal lung [51] FGF7 in mouse model, preventive [101] HGF in rats [100] FGF7 in mouse model, curative [101] …”
Section: Positive Effectsmentioning
confidence: 99%