Keratinization induced by air exposure in the reconstructed human epidermal model: An in vitro model of a cultured epithelial autograft

Hanada, Takao; Itahara, Yuichi; Katoh, Masakazu; Inoie, Masukazu; Hata, Ken‐ichiro

doi:10.1016/j.jbiosc.2014.02.019

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…, ; Hanada et al. , ). Our study confirmed only the construction of keratinocyte sheets, but differentiation of keratinocyte sheets was not assayed.…”

Section: Discussionmentioning

confidence: 99%

Harvesting epithelial keratinocyte sheets from temperature-responsive dishes preserves basement membrane proteins and improves cell survival in a skin defect model

Osada

Sekine

Soejima

et al. 2016

J Tissue Eng Regen Med

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Cultured epithelial autograft (CEA) therapy has been used in clinical applications since the 1980s. However, there are some issues related to this treatment that still remain unsolved. Enzymatic treatment is typically used in the collection of epithelial keratinocyte sheets, but it tends to break the adhesion and basement membrane proteins. It is thought that the loss of proteins after enzymatic treatment is responsible for the poor survival of transplanted cell sheets. Our laboratory has developed a temperature-responsive culture dish that does not require enzymatic treatment to harvest the cells. In this study, we compare morphological and survival results from rat epithelial keratinocyte cell sheets harvested by temperature-reducing treatment (TT sheets) against cell sheets harvested by enzymatic (dispase) treatment (DT sheets). TT sheets preserve keratin structure in better conditions and express higher levels of collagen IV and laminin 5 than DT sheets. In order to evaluate cell sheet survival after transplantation, we created an in vivo transplant model. Keratinocyte sheets obtained from GFP-positive animals were transplanted into athymic rats. The survival rate 7 days after transplantation of TT sheet was higher than that of DT sheets. Collagen IV and Laminin 5 expression was observed in the TT sheet transplantation group. These results indicate that the remaining basement membrane proteins are important for initial attachment and cell survival. We believe that the cell sheet harvesting method using temperature-responsive culture dishes provides superior cell survival and can solve one of the roadblocks in CEA therapy. Copyright © 2016 John Wiley & Sons, Ltd.

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“…, ; Hanada et al. , ). Our study confirmed only the construction of keratinocyte sheets, but differentiation of keratinocyte sheets was not assayed.…”

Section: Discussionmentioning

confidence: 99%

Harvesting epithelial keratinocyte sheets from temperature-responsive dishes preserves basement membrane proteins and improves cell survival in a skin defect model

Osada

Sekine

Soejima

et al. 2016

J Tissue Eng Regen Med

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“…In the USA, CEA has been usually applied with air exposure because of increasing take rates. To clarify the mechanism, Hanada has reported keratinization induced by air exposure with in vitro model of a CEA 6) . Other authors have investigated the skin structure histologically to compare normal skin, airexposed CEA, and non-air-exposed CEA.…”

Section: Discussionmentioning

confidence: 99%

“…They suggested that air exposure may be associated with the histological differentiation of CEA 7) . Therefore, to increase the take rate, a dry environment or air exposure after CEA transfer is recommended [6][7][8] . Air exposure of wounds is usually performed for several hours per day.…”

Section: Discussionmentioning

confidence: 99%

Aquacel® Ag Advantage Dressing is Effective in the Wound Management of Extensive Burns After Transplantation of Cultured Epidermal Autograft

Matsuura

Itano

Shoji-Pietraszkiewicz

et al. 2023

International Journal of Surgical Wound Care

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Cultured epidermal autografts have been used in recent years, despite the difficulty of treating extensive burns. Appropriate wound management after cultured epidermal autograft device transfer is also necessary. Physicians must ensure appropriate cultured epidermal autograft administration by managing the growth of microorganisms and meticulously changing patients' dressings. Moreover, cultured epidermal autograft requires a dry environment and exposure to air after engraftment. We provide a better dressing environment using the newly developed material Aquacel® Ag Advantage to manage wounds. A 71-year-old man sustained extensive burns on the right upper extremity, left arm, face and neck, chest, abdomen, anterior thighs, and right back. The total burn area was 38%, and the majority were full-thickness burns. Burn inhalation complicated the patient's condition. We performed consecutive surgical operations of debridement and skin grafting of the cultured epidermal autografts. We used Aquacel® Ag Advantage to manage the burn wounds after cultured epidermal autograft transfer, which helped control the growth of the resistant microbes Pseudomonas aeruginosa, and prevented adherence when changing dressings. This approach also absorbs the extra exudate, thereby keeping the surface of the cultured epidermal autograft dry. This innovative dressing approach may substitute air exposure following cultured epidermal autograft, hence increasing the cultured epidermal autograft take ratio.

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“…CEA is prepared using Green’s technique; in brief, a small skin biopsy was taken, and keratinocytes were separated and cultured with irradiated 3T3 cells. 8 , 9 CEA has been widely used for the treatment of severely burned patients for decades. 9 In Japan, CEA was approved and covered by public health care insurance for the treatment of GCMN in 2016.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Cultured Epithelial Autograft after Curettage for Giant Melanocytic Nevus of the Head

Maeda

Morimoto

Kakudo

et al. 2018

Plastic and Reconstructive Surgery - Global Open

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Keratinization induced by air exposure in the reconstructed human epidermal model: An in vitro model of a cultured epithelial autograft

Cited by 7 publications

References 10 publications

Harvesting epithelial keratinocyte sheets from temperature-responsive dishes preserves basement membrane proteins and improves cell survival in a skin defect model

Harvesting epithelial keratinocyte sheets from temperature-responsive dishes preserves basement membrane proteins and improves cell survival in a skin defect model

Aquacel® Ag Advantage Dressing is Effective in the Wound Management of Extensive Burns After Transplantation of Cultured Epidermal Autograft

Efficacy of Cultured Epithelial Autograft after Curettage for Giant Melanocytic Nevus of the Head

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