Keragaman genotipik entok (Cairina moschata) berdasarkan polimorfisme protein darah

Abstract

<p class="MDPI17abstract"><strong>Objective: </strong>The aim of this study was to identified genetic diversity of muscovy duck in Central Java through blood protein polymorphisms by using electrophoresis method.</p><p class="MDPI17abstract"><strong>Methods: </strong>Blood sample was collected from a total of 60 muscovy ducks from the districts of Demak, Magelang and Pekalongan, 20 samples each. The Electrophoresis Polyacrylamide Gel of Electrophoresis–Thin Layer Electrophoresis (PAGE-TLE) was used in this study. Parameters observed were protein of albumin (Alb), ceruloplasmin (Cp), transferrin (Tf) and amylase-I (Am-I). Gene frequency, heterozygosity (Ho) and genetic distance were analyzed by using DISPAN program.<strong></strong></p><p class="MDPI17abstract"><strong>Results</strong><strong>: </strong>The results showed that the Alb, Cp, Tf and Am-I of muscovy duck showing polymorphic characters. Gene frequency of Alb<sup>B</sup> (0.61) was higher compared to Alb<sup>A</sup> (0.39). Gene frequency of Cp<sup>A</sup> (0.62) was higher than the Cp<sup>B</sup> (0.38). Furthermore, gene frequency of Tf<sup>A</sup> and Tf<sup>B</sup> were 0.32 and 0.68, respectively as well as gene frequency of Am-I<sup>A</sup> (0.82) was higher than Am-I<sup>B</sup> (0.18). Muscovy duck population of Demak showed higher Ho value for Alb and Am-I, whereas Magelang showed higher value for Cp and Tf. Population of muscovy duck of Demak showed closer genetic distance to Pekalongan than Magelang.<strong></strong></p><strong>Conclusions: </strong>The protein blood locus of three population of muscovy duck in Central Jawa showed polymorphic characters.

Morphometric characterization and effect of growth hormone (GH) gene polymorphism on growth traits of Kerinci duck (Anas platyrhynchos)

“…The outcomes of this observe are not a lot exclusive from the research of [23], who stated that diversity of GH genes in exon 1, which was cut with the MboII enzyme, showed that the GH gene of Sikumbang Janti ducks was polymorphic characterized by the appearance of genotypes (+/+), (+/-), and (-/-) and two alleles + and -. Sutopo et al [26] stated that the population of Demak and Pekalongan ducks found two alleles on the GH gene, (+) and (-), which formed homozygous characters (+/+) and (-/-) and heterozygous (+/) characters. This condition indicates that the genotypes of Kerinci ducks vary.…”

Genetic diversity and phylogenetic study of Ongole Grade cattle population in Central Java based on blood protein polymorphism

“…So, it will greatly assist in designing livestock breeding programs. Blood protein analysis also can be used for animal physiological characteristic status (Utomo et al 2017), immunogenetic markers (Hrinca 2015;Tothova et al 2016), and identification of phylogenetical relationships (Nigussie et al 2016;Safran et al 2017;Sutopo et al 2021). Research on the genetic diversity of Ongole Grade cattle has been done by Sutopo et al (2001) in Java; Hartatik et al (2018) in Kebumen; Sutiyono et al (2018) in Rembang; Sutarno et al (2019) in Wonogiri.…”