Kefir alters craniomandibular bone development in rats fed excess dose of high fructose corn syrup

Ekici, Ömer; Aslan, Esra; Güzel, Hilal; Korkmaz, Ömer Adil; Sadı, Gökhan; Gurol, A M; Boyacı, Mehmet Gazi; Pektaş, Mehmet Bilgehan

doi:10.1007/s00774-021-01273-8

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…We have previously shown [Ekici et al, 2022a] that kefir protects young rats against the HFCS-induced inflammation of the masseter muscle and gingival tissue, as well as from the altered metabolic parameters that accompany this inflammation. We also showed the preventive effects on HFCS-induced bone loss in the craniofacial region of rat models [Ekici et al, 2022b]. Herein, we aimed to investigate the importance of apoptosis on adipocytes and adipogenesis, and the effects of kefir consumption on high-fructose corn syrup-induced adipogenesis at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed Rats

Aslan¹,

Sadı²,

Güzel³

et al. 2023

Pol. J. Food Nutr. Sci.

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Consumption of high-fructose corn syrup (HFCS) in the diet is a causal factor in the development of abdominal obesity; however, the molecular mechanism behind this association is still up for debate. This study evaluated the metabolic disturbances that are caused by HFCS on adipose tissue as well as the possibility of kefir as a therapy to prevent these metabolic disturbances. Male Wistar rats were divided into four groups: control, kefir, HFCS, and HFCS+kefir. HFCS (20%, w/v) was given in drinking water and kefir (1 mL/100 g body weight) by gastric gavage daily for 8 weeks. Levels of insulin signaling, inflammation, and apoptosis-associated proteins of adipose tissues were determined with Western blot and immunohistochemical techniques. Gene expressions were evaluated with semi-quantitative real-time polymerase chain reaction (qRT-PCR). The indirect terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) method was used to assess changes in apoptotic cells, and hematoxylin/eosin staining to determine adipocyte number and diameter. Accordingly, HFCS boosted protein kinase B (Akt) and p-Akt while reducing nuclear factor κB (NF-κB), and tumor necrosis factor alpha (TNFα) levels and kefir treatment restored Akt induction in HFCS-treated rats despite raising NF-κB, and TNFα. Increased expression of Akt and B-cell lymphoma-2 gene (Bcl2) was contrasted with decreased expression of Nfkb, Tnfa, tumor protein 53 gene (p53), and caspase-8 gene (Casp8). Furthermore, while there was a marked reduction in TUNEL-positive cells in the HFCS group, the number of such cells was greater in the HFCS+kefir group. These results show that HFCS intake suppresses apoptosis in adipose tissues, which may be responsible for tissue development and abdominal obesity and may be reversed with kefir administration due to the activation of apoptosis-associated genes and proteins.

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Section: Introductionmentioning

confidence: 99%

Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed Rats

Aslan¹,

Sadı²,

Güzel³

et al. 2023

Pol. J. Food Nutr. Sci.

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Unlocking Innovations: Exploring the Role of Kefir in Product Development

Manjunatha,

Bhattacharjee,

Flores

2024

Curr Food Sci Tech Rep

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Purpose of Review The current review explores kefir's role in product development, investigating production methods, microbial composition, and health benefits across traditional and industrial processes. Variations in fermentation techniques and microbial diversity, highlighting milk and water kefir's potential as functional foods catering to diverse consumer preferences, have been evaluated. Recent Findings Recent literature underscores the diverse microbial community in kefir grains, which contributes to texture, flavor, and biological properties, including antimicrobial, anticarcinogenic, anti-inflammatory, and antioxidant effects. Kefir-based products are diverse and include traditional fermented milk drinks and innovative formulations like kefir cheese and powdered starter cultures, with emerging techniques such as spray drying resolving issues in shelf life and storage stability. Summary The beneficial aspect of Kefir makes it a promising avenue for innovation in product development by exploiting fermentation matrices and augmenting it with vitamins, minerals, and probiotics thereby enhancing its functional properties for gut health and diverse diet. Emerging technologies have been critical in tackling the challenges of shelf life and packaging costs. Continuing research and innovation addresses kefir's potential as a functional food, catering to the growing demand for health-oriented and sustainable products.

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Mangiferin Induces Post-Implant Osteointegration in Male Diabetic Rats

Ongan,

Ekici,

Sadi

et al. 2024

Medicina

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Background and Objectives: Hyperglycemia is known to undermine the osteointegration process of implants. In this study, the effects of mangiferin (MF) on the post-implant osteointegration process in a type-II diabetes model were investigated molecularly and morphologically. Materials and Methods: Sprague Dawley male rats were divided into three groups: control, diabetes, and diabetes + MF. All animals were implanted in their tibia bones on day 0. At the end of the 3-month experimental period, the animals’ blood and the implant area were isolated. Biochemical measurements were performed on blood samples and micro-CT, qRT-PCR, histological, and immunohistochemical measurements were performed on tibia samples. Results: MF significantly improved the increased glucose, triglyceride-VLDL levels, and liver enzymes due to diabetes. By administering MF to diabetic rats, the osteointegration percentage and bone volume increased while porosity decreased. DKK1 and BMP-2 mRNA expressions and OPN, OCN, and OSN mRNA–protein expressions increased by MF administration in diabetic rats. Additionally, while osteoblast and osteoid surface areas increased with MF, osteoclast and eroded surface areas decreased. Conclusions: The findings of our study indicate that MF will be beneficial to the bone-repairing process and osteointegration, which are impaired by type-II diabetes.

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Kefir alters craniomandibular bone development in rats fed excess dose of high fructose corn syrup

Cited by 3 publications

References 36 publications

Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed Rats

Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed Rats

Unlocking Innovations: Exploring the Role of Kefir in Product Development

Mangiferin Induces Post-Implant Osteointegration in Male Diabetic Rats

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