Keeping time in the dark: Potato diel and circadian rhythmic gene expression reveals tissue‐specific circadian clocks

Abstract

The circadian clock is an internal molecular oscillator and coordinates numerous physiological processes through regulation of molecular pathways. Tissue-specific clocks connected by mobile signals have previously been found to run at different speeds in Arabidopsis thaliana tissues. However, tissue variation in circadian clocks in crop species is unknown. In this study, leaf and tuber global gene expression in cultivated potato under cycling and constant environmental conditions was profiled. In addition, we used a circadian-regulated luciferase reporter construct to study tuber gene expression rhythms. Diel and circadian expression patterns were present among 17.9% and 5.6% of the expressed genes in the tuber. Over 500 genes displayed differential tissue specific diel phases. Intriguingly, few core

Elucidation of the interactome of the sucrose transporter StSUT4: sucrose transport is connected to ethylene and calcium signalling

“…The sub-cellular localization of plant sucrose transporters is highly dynamic and affected by various external factors such as pH, temperature, oligomerization, ions, the substrate sucrose, inhibitors such as Brefeldin A (BFA) or cycloheximide (CHX), and biotic interactions such as colonization by mycorrhizal fungi, etc. ( Krügel et al ., 2008 , 2012 ; Liesche et al , 2008 , 2010 ; Bitterlich et al , 2014 ; Garg et al , 2020 ; Hansch et al , 2020 ). Sub-cellular localization of sucrose transporter might not only be affected by homo-oligomerization, but also by other interaction partners.…”

“…A very recent study identifies StSUT4 , together with the sugar transporter S UGARS W ILL E VENTUALLY BE E XPORTED T RANSPORTER ( SWEET11) as core circadian clock genes in below-ground storage organs, showing a robust circadian transcriptional rhythm in detached tubers independent from leaves, with important implication for the regulation of tuberization in potato crop plants ( Hoopes et al , 2022 ). These transcriptomic data further strengthen the hypothesis of StSUT4 as a key component of the circadian clock in potato tubers.…”

Inbreeding effects on the performance and genomic prediction for polysomic tetraploid potato offspring grown at high Nordic latitudes

“…Tuberization, affected by daylength and early maturity and plant growth, as measured by its canopy and vine senescence, are complex quantitative traits and their multi-genic nature (e.g. circadian clone genes regulating tuberization [21,22], or phytochromes light receptors [23], among others) makes these characteristics difficult to evaluate in the early stages of the potato breeding cycle, particularly in long day sites with short cropping season. Furthermore, daylength fluctuations promote flowering time and tuber differentiation plus bulking in potato, both of which, as summarized by Rodríguez-Falcón et al [24], share common regulatory pathways.…”

Diurnal transcriptome dynamics reveal the photoperiod response of Pyrus

“…In recent decades, the molecular mechanism of photoperiod regulation of plant development has been deciphered in several plants. Recently, photoperiod regulation of other life processes has become a popular research topic, and many key genes involved in regulatory networks have been identified (Hoopes et al, 2022; Oravec & Greenham, 2022). Here, we provide an approach for exploring diurnally expressed genes related to pear growth and their role in regulating plant fitness.…”