Keeping the Serpin Machine Running Smoothly: Figure 1.

Gettins, Peter G.W.

doi:10.1101/gr.168900

Cited by 38 publications

(25 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Observations suggest that serine protease inhibitors exert inhibitory activity against proteases in the control of events associated with inflammatory reactions and connective tissue turnover (6,7). Recently it has been reported that serpinA3 is aberrantly expressed in metastatic melanoma (18).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of the serine protease inhibitor serpinA3 in human colorectal adenocarcinomas

et al. 2011

View full text Add to dashboard Cite

Abstract. Proteases facilitate a number of steps in cancer progression. The serine protease inhibitors (serpins) are a protein superfamily with inhibitory activity against proteases. One of these proteases, serpinA3, appears to have a multifaceted role and is associated with inflammatory reactions, Alzheimer's disease, malignant melanoma and gastric cancer. To gain insight into the potential effect of serpinA3 on colorectal cancer (CRC) we determined whether serpinA3 is altered in colorectal tissue or plasma in CRC patients. Collectively, by using ELISA we noted a significantly lower serpinA3 level in cancer tissue compared to paired normal tissue. Moreover, the tumour serpinA3 level tended to be higher in disseminated disease as compared to localised disease. No significant difference in the plasma levels of serpinA3 was noted in the patients when compared to the controls. However, plasma serpinA3 and C-reactive protein (marker of inflammation) in the CRC patients and controls were significantly positively correlated. To confirm and detect localization of serpinA3 expression, immunohistochemistry was performed. Immunohistochemistry showed heterogeneous immunoreactivity in epithelial cells in the cancer and normal tissue and extracellular staining within bands of stroma as well as in some stromal cells. A Taq Man system was used to investigate a single nucleotide polymorphism (rs4934) in the serpinA3 signal sequence gene with supposed effect on serpinA3 secretion and expression. No significant difference was observed between CRC and control subjects regarding genotype and allelic distributions, nor were associations noted between clinical characteristics and serpinA3 levels. In conclusion, an altered serpinA3 concentration in CRC tissue may be a potential biomarker in CRC progression. SerpinA3 concentrations in plasma appear to be correlated with systemic inflammation, but do not appear to be specific to CRC patients. Further studies are warranted to improve our understanding of the role of serpinA3 in CRC.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Serine protease inhibitors (serpins) belong to a protein superfamily with inhibitory activity against proteases. These serpins are involved in various physiological processes and in the regulation of events associated with inflammatory reactions and connective tissue turnover (6,7).…”

Section: Introductionmentioning

confidence: 99%

Expression of the serine protease inhibitor serpinA3 in human colorectal adenocarcinomas

et al. 2011

View full text Add to dashboard Cite

show abstract

“…It belongs to a family of intracellular and extracellular serine and cysteine proteinase inhibitors called serpins. Their target enzymes regulate very diverse processes, including apoptosis, inflammation and neoplasia (Gettins, 2000). Its expression has been shown to be strongly induced by IFN-c and in vivo by intracellular bacteria such as Mycobacterium bovis bacille Calmette-Guérin (BCG), L. monocytogenes and Salmonella typhimurium, and by bacterial products like LPS or peptidoglycan (Hamerman et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling of host responses in mouse lungs following aerosol infection with type A Francisella tularensis

Andersson

Hartmanová

KuoLee

et al. 2006

Journal of Medical Microbiology

View full text Add to dashboard Cite

Tularaemia caused by inhalation of type A Francisella tularensis bacteria is one of the most aggressive infectious diseases known, but the reasons for the very rapid spread of the organism from the lungs to internal organs and the ensuing mortality are unknown. The present study used the mouse model to examine in detail the host immune response in the lung. After an aerosol challenge with 20 c.f.u. of the type A strain FSC033, all mice developed clinical signs of severe disease, showed weight loss by day 4 of infection and died the next day. Histopathological findings in the lung revealed acute inflammation and intense vasculitis and perivasculitis on day 4. Gene transcriptional changes in the mouse lung samples were examined on days 1, 2 and 4 of infection using a cDNA microarray with 20 600 mouse clones representing 18 500 genes. In total, 424 genes were found to be differentially expressed, some of which were both up-and downregulated at different time points, 192 of which were upregulated and 234 of which were downregulated for at least one time point. A high percentage of selected genes identified by the microarray analysis were confirmed to be differentially regulated by quantitative real-time PCR. Categorization of the differentially expressed genes showed that those preferentially involved in host immune responses were activated extensively on day 4 but hardly or not at all on days 1 and 2. Further analysis revealed that several of the genes upregulated on day 4 are known to depend on gamma interferon or tumour necrosis factor alpha for their regulation. In keeping with this finding, tumour necrosis factor alpha and gamma interferon levels were found to be increased significantly in bronchoalveolar lavage on day 4.

show abstract

“…One of the most significantly induced genes was serpina 3 g, a member of the mouse serpins family (Table1, section VI). Serpins are serine proteinase inhibitors that are irreversible suicide inhibitors of protease enzymes regulating processes of coagulation, fibrinolysis, complement activation, angiogenesis, apoptosis, inflammation, and neoplasia (60). An important cytokine family to be included under this title is the transforming growth factor family.…”

Section: Section V)mentioning

confidence: 99%

Immune Response toMycobacterium tuberculosisand Identification of Molecular Markers of Disease

Gonzalez-Juarrero

Kingry²,

Ordway³

et al. 2009

Am J Respir Cell Mol Biol

View full text Add to dashboard Cite

Keeping the Serpin Machine Running Smoothly: Figure 1.

Cited by 38 publications

References 20 publications

Expression of the serine protease inhibitor serpinA3 in human colorectal adenocarcinomas

Expression of the serine protease inhibitor serpinA3 in human colorectal adenocarcinomas

Transcriptional profiling of host responses in mouse lungs following aerosol infection with type A Francisella tularensis

Immune Response toMycobacterium tuberculosisand Identification of Molecular Markers of Disease

Contact Info

Product

Resources

About