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Abstract

Pyridoxine (vitamin B 6 ) is a water-soluble vitamin commonly found in food and also provided using dietary supplements. It plays an important role in the biosynthesis of neurotransmitters and maintaining healthy levels of homocysteine, the amino acid in the blood, gluconeogenesis, immune function, haemoglobin formation, etc. This study was performed to determine the impact of the Trivedi Effect ® -Biofield Energy Healing Treatment on the structural properties and the isotopic abundance ratio of pyridoxine hydrochloride using LC-MS and GC-MS spectroscopy. Pyridoxine HCl sample was divided into two parts, one part of pyridoxine was considered as control (no Biofield Energy Treatment was provided), while the second part was treated with the Trivedi Effect ® -Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Alice Branton and termed as a treated sample. The LC-MS spectra of both the control and Biofield Energy Treated pyridoxine hydrochloride samples at retention time 2.33 minutes exhibited the mass of the protonated molecular ion peak [M+H] + at m/z 170 (calculated for C 8 H 12 NO 3 + , 170.08) with 100% base peak intensity in the MS spectrum in +ve ion mode was found to be pyridoxine. The LC-MS based isotopic abundance ratios of PM+1/PM and PM+2/PM in the treated pyridoxine were significantly increased by 20.45% and 116.67%, respectively compared with the control sample. Thus, 13 C, 2 H, 15 N, 17 O, and 18 O contributions from (C 8 H 12 NO 3 ) + to m/z 171 and 172 in the treated sample were significantly increased compared with the control sample. Similarly, in the GC-MS chromatograms, the peak area% of the treated sample was increased by 4.06% compared to the control sample. But the mass peak intensity of the treated pyridoxine at m/z 151 was significantly decreased by 14.16% compared to the control sample. The isotopic abundance ratios of P M+1 /P M ( 2 H/ 1 H or 13 C/ 12 C or 15 N/ 14 N or 17 O/ 16 O), P M+2 /P M ( 18 O/ 16 S), and peak area% in the treated pyridoxine were significantly increased compared to the control sample. It can be assumed that the changes in isotopic abundance, peak area%, and mass peak intensities could be due to changes in nuclei possibly via the interference of neutrino particles controlled by The Trivedi Effect ® -Consciousness Energy Healing Treatment. The new form of pyridoxine HCl would be better for designing novel pharmaceutical formulations which would be more soluble, absorbable, and bioavailable, that might offer better therapeutic response for the prevention and treatment of vitamin B 6