Kdr mutations in Triatoma infestans from the Gran Chaco are distributed in two differentiated foci: Implications for pyrethroid resistance management

Sierra, Ivana; Capriotti, Natalia; Fronza, Georgina; Mougabure‐Cueto, Gastón; Ons, Sheila

doi:10.1016/j.actatropica.2016.03.014

Cited by 38 publications

(36 citation statements)

References 15 publications

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“…However, it is important to mention that Sierra et al . () observed that T . infestans‐ resistant populations from Gran Chaco (ecoregion covering part of Argentina, Bolivia and Paraguay) have the kdr mutation at similar frequencies but have very different resistance ratios (Henry‐Halldin et al ., ).…”

Section: Discussionmentioning

confidence: 95%

“…The main cause of the extremely high levels of resistance in T . infestans seems to be the appearance of kdr mutations, L1014F and L925I, the only two mutations found in triatomines so far, affecting the target site of pyrethroids, the voltage‐gated sodium channel (Fabro et al ., ; Capriotti et al ., ; Roca‐Acevedo et al ., ; Sierra et al ., ). However, it is important to mention that Sierra et al .…”

Section: Discussionmentioning

confidence: 97%

“…The detection of resistant populations of T. infestans confirms the potential of triatomines to develop high lev-els of resistance. The main cause of the extremely high levels of resistance in T. infestans seems to be the appearance of kdr mutations, L1014F and L925I, the only two mutations found in triatomines so far, affecting the target site of pyrethroids, the voltage-gated sodium channel (Fabro et al, 2012;Capriotti et al, 2014;Roca-Acevedo et al, 2015;Sierra et al, 2016). However, it is important to mention that Sierra et al (2016) observed that T. infestans-resistant populations from Gran Chaco (ecoregion covering part of Argentina, Bolivia and Paraguay) have the kdr mutation at similar frequencies but have very different resistance ratios (Henry-Halldin et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

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NovelKdrmutations (K964R and A943V) in pyrethroid‐resistant populations ofTriatoma mazzottiiandTriatoma longipennisfrom Mexico and detoxifying enzymes

et al. 2018

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Although having five different ways of transmission the vector-borne is the principal way of transmission of Chagas disease, which involves insects of the subfamily Triatominae (Hemiptera: Reduviidae). Nineteen of the 31 species that occur in Mexico are associated with humans, and all are capable of transmitting the disease. Pyrethroids are the insecticides recommended for the control of these vectors in Mexico. We determined the susceptibility to the pyrethroids deltamethrin and permethrin of peridomestic populations of Triatoma mazzottii Usinger and two populations of Triatoma longipennis Usinger in comparison with a reference strain for each species. Bioassays were performed for the determination of the LD for both field populations and reference strains. A maximum of 27 fold resistance to deltamethrin was observed in T. mazzottii, meanwhile, for permethrin, T. longipennis from Jalisco show the highest value of 3.19 fold. There was significantly increased activity of esterases in field populations in comparison with their corresponding reference strain. The results of the search of kdr mutations related to the resistance to deltamethrin and permethrin in the evaluated species show the presence of mutations in the field populations, as is the case with individuals of T. mazzottii, for which the mutation was found A943V, and for the two populations of T. longipennis included in this study, we report the presence of the kdr mutation K964R. Evaluation of the various mechanisms involved in resistance to pyrethroids in triatomines from Mexico could guide us to the real justification for insecticide resistance monitoring.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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NovelKdrmutations (K964R and A943V) in pyrethroid‐resistant populations ofTriatoma mazzottiiandTriatoma longipennisfrom Mexico and detoxifying enzymes

et al. 2018

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“…The most relevant and most documented resistance mechanisms in insects (i.e. reduced penetration, enhanced metabolism and modified site of action) were described in pyrethroid-resistant T. infestans (Picollo et al, 2005;Pedrini et al, 2009;Germano et al, 2012;Sierra et al, 2016). Recently a one-point mutation (L925I) associated with resistance in the gene responsible for the voltage-gated sodium channel (i.e.…”

Section: Discussionmentioning

confidence: 99%

Excretion/defecation patterns in Triatoma infestans populations that are, respectively, susceptible and resistant to deltamethrin

Lobbia

Calcagno

Mougabure‐Cueto

2018

Medical Vet Entomology

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Pyrethroid resistance has been detected in Triatoma infestans (Klug) (Hemiptera: Reduviidae) specimens from different areas of Argentina and Bolivia. Genes conferring resistance can have a pleiotropic effect with epidemiological and evolutionary consequences. This research studied excretion/defecation patterns in deltamethrin-resistant T. infestans in order to elucidate its biological performance, adaptive consequences and role in the transmission of Chagas' disease. One deltamethrin-susceptible strain and two deltamethrin-resistant strains were used. Fifth-instar nymphs were fed ad libitum and their defecations recorded during and after the first or second feeding in the stadium. Resistant insects began to defecate later, defecated less, showed a lower proportion of defecating individuals and lower defecation indices compared with susceptible insects during the first hour after feeding. The number of bloodmeals in the stadium did not affect the main variables determining the pattern of defecation. The present study suggests that alterations in the excretion/defecation pattern in resistant insects entail an adaptive cost and, considering only this pattern, determine a lower capacity for transmission of Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) compared with susceptible insects.

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“…Later, a new pyrethroid resistance-conferring mutation (L925I) was identified in the sodium channel of T. infestans from El Mala, a little locality in the Argentinean Gran Chaco region. Unlike L1014F mutation that was identified in many insect species from different families, L925I mutation has been identified only in hemipterans (Capriotti, Mougabure-Cueto, Rivera-Pomar, & Ons, 2014;Sierra, Capriotti, Fronza, Mougabure-Cueto, & Ons, 2016).…”

Section: Target-site Insensitivitymentioning

confidence: 95%

Identifying Current and Missing Knowledge in the Control of Pyrethroid-Resistant Triatoma infestans, Vector of Chagas Disease

Acevedo¹,

Picollo²

2017

GJHS

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Triatomines are blood-sucking bugs that occur mainly in Latin America. They are vectors of Trypanosoma cruzi, the parasite that causes Chagas disease. Chemical control of Chagas disease´s vectors by using pyrethroid insecticides has been highly successful for the elimination of domestic infestation and consequently the reduction of the vector transmission. However, at the beginning of the 2000s a decrease in the effectiveness of the chemical control of triatomines was detected in several areas from Argentina and Bolivia, particularly in the Gran Chaco eco-region.During the last 15 years, several studies demonstrated the evolution of insecticide resistance in Triatoma infestans and established the presence of different toxicological profiles, the autosomal inherence of resistance, the biological costs of deltamethrin resistance, the expression of deltamethrin resistance thorough the embryonic development, and the main mechanisms of resistance (target-site insensitivity and metabolic detoxification of insecticides).The emergence of pyrethroid resistance coupled with the usual difficulties in sustaining adequate rates of insecticide applications emphasize the need of incorporating other tools for integrated vector and disease control, such as the proposal of the organo-phosphorus insecticide fenitrothion as an alternative chemical strategy for the management of the resistance because it was effective against pyrethroid-resistant populations in laboratory and semi-field trials.New studies on the current situation of presence and spread of resistant populations of triatomines and the acceptance of the use of alternative insecticides are critical requirements in the implementation of strategies for the management of resistance and for the rational design of campaigns oriented to reducing the vector transmission of Chagas' disease.

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Kdr mutations in Triatoma infestans from the Gran Chaco are distributed in two differentiated foci: Implications for pyrethroid resistance management

Cited by 38 publications

References 15 publications

NovelKdrmutations (K964R and A943V) in pyrethroid‐resistant populations ofTriatoma mazzottiiandTriatoma longipennisfrom Mexico and detoxifying enzymes

NovelKdrmutations (K964R and A943V) in pyrethroid‐resistant populations ofTriatoma mazzottiiandTriatoma longipennisfrom Mexico and detoxifying enzymes

Excretion/defecation patterns in Triatoma infestans populations that are, respectively, susceptible and resistant to deltamethrin

Identifying Current and Missing Knowledge in the Control of Pyrethroid-Resistant Triatoma infestans, Vector of Chagas Disease

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