KChIP-Like Auxiliary Subunits of Kv4 Channels Regulate Excitability of Muscle Cells and Control Male Turning Behavior during Mating in<i>Caenorhabditis elegans</i>

Chen, Xin; Ruan, Meiyu; Cai, Shi-Qing

doi:10.1523/jneurosci.3429-14.2015

Cited by 3 publications

(2 citation statements)

References 52 publications

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“…Most types of ion channels are found in C. elegans and are known to regulate worm behaviors 6 – 10 . Like their human homologs, C. elegans channels usually consist of pore-forming and accessory subunits 10 , 11 . Many mechanisms underlying the biogenesis of ion channels are conserved from C. elegans to humans 11 – 13 .…”

Section: Introductionmentioning

confidence: 99%

“…Like their human homologs, C. elegans channels usually consist of pore-forming and accessory subunits 10 , 11 . Many mechanisms underlying the biogenesis of ion channels are conserved from C. elegans to humans 11 – 13 . Based on these findings and cumulative knowledge regarding ion channel mutations associated with human diseases, we hypothesized that expression of disease-relevant human ion channels in C. elegans would disturb normal electrical signaling and hence cause phenotypic defects in the transgenic worms.…”

Section: Introductionmentioning

confidence: 99%

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Identification of small-molecule ion channel modulators in C. elegans channelopathy models

Jiang

Lü

et al. 2018

Nat Commun

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Ion channels are important therapeutic targets, but the discovery of ion channel drugs remains challenging due to a lack of assays that allow high-throughput screening in the physiological context. Here we report C. elegans phenotype-based methods for screening ion channel drugs. Expression of modified human ether-a-go-go-related gene (hERG) potassium channels in C. elegans results in egg-laying and locomotive defects, which offer indicators for screening small-molecule channel modulators. Screening in worms expressing hERGA561V, which carries a trafficking-defective mutation A561V known to associate with long-QT syndrome, identifies two functional correctors Prostratin and ingenol-3,20-dibenzoate. These compounds activate PKCε signaling and consequently phosphorylate S606 at the pore region of the channel to promote hERGA561V trafficking to the plasma membrane. Importantly, the compounds correct electrophysiological abnormalities in hiPSC-derived cardiomyocytes bearing a heterozygous CRISPR/Cas9-edited hERGA561V. Thus, we have developed an in vivo high-throughput method for screening compounds that have therapeutic potential in treating channelopathies.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of small-molecule ion channel modulators in C. elegans channelopathy models

Jiang

Lü

et al. 2018

Nat Commun

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The Biosynthesis and Folding of Oily Peptide Chains

Cai

2019

Advances in Membrane Proteins

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Sexual Dimorphism and Sex Differences in Caenorhabditis elegans Neuronal Development and Behavior

2018

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As fundamental features of nearly all animal species, sexual dimorphisms and sex differences have particular relevance for the development and function of the nervous system. The unique advantages of the nematode have allowed the neurobiology of sex to be studied at unprecedented scale, linking ultrastructure, molecular genetics, cell biology, development, neural circuit function, and behavior. Sex differences in the nervous system encompass prominent anatomical dimorphisms as well as differences in physiology and connectivity. The influence of sex on behavior is just as diverse, with biological sex programming innate sex-specific behaviors and modifying many other aspects of neural circuit function. The study of these differences has provided important insights into mechanisms of neurogenesis, cell fate specification, and differentiation; synaptogenesis and connectivity; principles of circuit function, plasticity, and behavior; social communication; and many other areas of modern neurobiology.

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KChIP-Like Auxiliary Subunits of Kv4 Channels Regulate Excitability of Muscle Cells and Control Male Turning Behavior during Mating inCaenorhabditis elegans

Cited by 3 publications

References 52 publications

Identification of small-molecule ion channel modulators in C. elegans channelopathy models

Identification of small-molecule ion channel modulators in C. elegans channelopathy models

The Biosynthesis and Folding of Oily Peptide Chains

Sexual Dimorphism and Sex Differences in Caenorhabditis elegans Neuronal Development and Behavior

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