Katecholaminhaltige Neurone in der neuroendokrinen Steuerung Prinzip, Anwendung der Mikrofluorimetrie

Lichtensteiger, W.

doi:10.1016/s0079-6336(70)80010-9

Cited by 51 publications

(15 citation statements)

References 250 publications

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“…Lichtensteiger, 1969bLichtensteiger, , 1970a. For instance the mean of the logarithmically transformed frequency distribution of relative fluorescence intensity (m) was 3-497 24 hr after 10 ,ag oestradiol-dipropionate and 3-462 after treatment with a high dose of oestradiol-dipropionate (100,ug) and progesterone (2 mg) for 2 days (logarithmical means from experiments by 68 TUBERAL CATECHOLAMINE NEURONES Lichtensteiger et al 1969; the second logarithm is unpublished).…”

Section: Resultsmentioning

confidence: 98%

“…2). The initial response of the nerve cell body turned out to be a very fast one: after 10 min of stimulation, fluorescence intensities higher than the maxima of the normal oestrous cycle were observed (for logarithmical means, see Lichtensteiger, 1970a). They differ markedly from the low intensity levels of the animals in which the electrode had been left in place for 10 min without stimulation.…”

Section: Discussionmentioning

confidence: 96%

“…As has been shown in detail for the arcuate nucleus, these distributions can be closely approximated by the logarithmically normal (lognormal) type of distribution (Lichtensteiger, 1970a). Especially when higher mean fluorescence intensities occurred, the lognormal four-parameter distribution, which allows for the existence of an upper and lower limit for the distribution (Johnson, 1949), appeared to yield a somewhat better approximation than the lognormal two-parameter distribution, which is unbounded with respect to positive infinity.…”

Section: Microfluorimetrymentioning

confidence: 88%

“…The microscopic and electronic instrumentation employed for microfluorimetry in incident light has been described in detail (Lichtensteiger 1969b(Lichtensteiger , 1970a. The illumination was slightly modified: the new Ploem illuminator was used for reflexion of the exciting light through the objective to the preparation.…”

Section: Microfluorimetrymentioning

confidence: 99%

“…This region is known for its role in neuroendocrine regulation. As the tuberal catecholamine-containing nerve cells are thought to be involved in the control of anterior pituitary function (see Lichtensteiger, 1970a, for review), it seemed of interest to investigate whether they could be influenced by stimulation of the medial preoptic area. The experiments were conducted on ovariectomized rats treated for 1 day with oestrogen and progesterone.…”

Section: Introductionmentioning

confidence: 99%

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Effect of electrical stimulation on the fluorescence intensity of catecholamine‐containing tuberal nerve cells*

Lichtensteiger¹

1971

The Journal of Physiology

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SUMMARY1. By means of a microfluorimetric technique, the intensity of the catecholamine fluorescence was measured in nerve cells of the arcuate and periventricular hypothalamic nuclei of ovariectomized rats pre-treated with oestrogen and progesterone. In such animals the fluorescence intensity is in the range of the lowest intensities that were observed during the oestrous cycle.2. Unilateral intermittent electrical stimulation of the arcuate nucleus or of the medial preoptic area induced an acute increase of the fluorescence intensity in the cell bodies of the tuberal catecholamine-containing nerve cell group. The change was already manifest 5 min after the onset of stimulation and within 10 min the mean fluorescence intensity reached levels that are among the highest so far observed in this cell group. Prolonged stimulation of the medial preoptic area led to a biphasic response, as shown by a decrease in intensity between 30 and 60 min of stimulation.3. The increase in fluorescence intensity caused by stimulation of the arcuate nucleus was completely prevented by pre-treatment with the tyrosine hydroxylase inhibitor ac-methyl-tyrosine. Thus, the effect appears to be due to an enhancement of amine synthesis.4. The intensity values found after synthesis inhibition were used for a preliminary estimation of the catecholamine turnover time in the nerve cell bodies of the arcuate nucleus.5. In conclusion, electrical stimulation appears to induce a charac-

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Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 96%

Section: Microfluorimetrymentioning

confidence: 88%

Section: Microfluorimetrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of electrical stimulation on the fluorescence intensity of catecholamine‐containing tuberal nerve cells*

Lichtensteiger¹

1971

The Journal of Physiology

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Catecholaminergic Brain Stem Regulatory Systems

Björklund

Lindvall

1986

Comprehensive Physiology

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Toxicity of 6‐hydroxydopamine and dopamine for dopaminergic neurons in culture

Michel

Hefti

1990

J of Neuroscience Research

317

126

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Toxicity of 6-hydroxydopamine (6-OHDA) and dopamine were studied in cultures of dissociated fetal rat mesencephalic cells. To assess survival and function of dopaminergic cells we quantified the number of tyrosine hydroxylase-positive cells and measured dopamine uptake. Non-dopaminergic cells were monitored by counting the number of cells visible with phase-contrast microscopy and measuring GABA uptake. 6-OHDA, in contrast to MPP+, which selectively destroyed dopaminergic neurons, was found to be a non-selective neurotoxin in this culture system. Between 10 and 100 microM, dopaminergic and non-dopaminergic cells were destroyed. At concentrations higher than 100 microM, i.e., concentrations frequently used to lesion catecholaminergic neurons in vivo, 6-OHDA resulted in structural fixation and loss of viability of dopaminergic and non-dopaminergic cells. Dopamine produced the same actions at slightly higher concentrations. One hundred to 300 microM was toxic for all cell types, and concentrations above 300 microM resulted in fixation. The findings suggest that 6-OHDA cannot be considered a selective toxin for catecholaminergic neurons in vitro. The demonstrated toxicity of dopamine tends to support speculations that processes related to dopamine metabolism may play a role in the pathogenesis of Parkinson's disease.

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Katecholaminhaltige Neurone in der neuroendokrinen Steuerung Prinzip, Anwendung der Mikrofluorimetrie

Cited by 51 publications

References 250 publications

Effect of electrical stimulation on the fluorescence intensity of catecholamine‐containing tuberal nerve cells*

Effect of electrical stimulation on the fluorescence intensity of catecholamine‐containing tuberal nerve cells*

Catecholaminergic Brain Stem Regulatory Systems

Toxicity of 6‐hydroxydopamine and dopamine for dopaminergic neurons in culture

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