Katalytische Aktivität der Serumlipase im kontinuierlichen titrimetrischen Test in Abhängigkeit von der Temperatur

Abstract

The effect of temperature on the catalytic activity of serum lipase in the continuous titrimetric assay Summary:The catalytic activity of the pancreatic lipase in serum was measured between 20 °C and 37 °C by the continuous titrimetric assay. As the temperature was increased, the time course of Substrate hydrolysis showed an increasing tendency to becorne non-linear. The degree of non-linearity and its time of onset depended on the sample, the volurne of sefurn in the assay, and the composition of the triglyceride Substrate mixture. Twenty seven series of investigations between 20 °C and 32 °C (maximal) showed a Q !0 value of l .45 (s = 0.03) and ari activation energy of = 27.42 ± 0.565 kJ/mol (6550 ± 135 cal/mol; ± s).At low assay temperature, the addition of colipase caused a slight deterioration of the reaction kinetics, whereas at higher temperatüres it caused a marked improvement in the time course of Substrate hydrolysis. We were unable, however, to identify a fixed colipase concentration that would promote a linear reaction irrespective of sample origih and Volume.

Macro Lipase — A New Member of the Family of Immunoglobulin-Linked Enzymes

“…We therefore only calculated apparent energies of activation for the temperature interval 25 -34 °C. The activation energies compare well with recently published results (22), which were obtained using different assays. Determinations of the energies of activation by the assays used in our study (tab.…”