Karyotypic patterns of seven species of molossid bats (Molossidae, Chiroptera)

Morielle‐Versute, Eliana; Varella-Garcia, M.; Taddei, Valdir A.

doi:10.1159/000134154

Cited by 30 publications

(36 citation statements)

References 4 publications

(9 reference statements)

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“…Comparisons of chromosome banding patterns support the hypothesis that Robertsonian and tandem chromosomal fusions were the main events in the karyotype evolution of Chiroptera, and the reduced chromosomal number found in some species of phyllostomid and molossid bats may have resulted from such events (Morielle-Versute et al, 1996;de Faria and Morielle-Versute, 2006;Ao et al, 2006Ao et al, , 2007Mao et al, 2008).…”

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confidence: 61%

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Short CommunicationNew insights into telomeric DNA sequence (TTAGGG)n location in bat chromosomes

Faria¹,

Marchesin²,

Moreira³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. Molossidae species, Cynomops abrasus (2n = 34, fundamental number, FN = 64), Eumops auripendulus (2n = 42, FN = 62), Molossus rufus (2n = 48, FN = 64), Molossops temminckii (2n = 48, FN = 64), and Nyctinomops laticaudatus (2n = 48, FN = 64), and Phyllostomidae species, Phyllostomus discolor (2n = 32, FN = 60), have karyotypes with different chromosome and fundamental numbers, different localization of constitutive heterochromatin, and different numbers and location of nucleolar organizer regions (NORs). Fluorescence in situ hybridization with a human probe of the telomeric sequence (TTAGGG) n produced fluorescent signals in telomeric regions of the six bat species' chromosomes; in E. auripendulus, pericentromeric signals were also observed in the acrocentric and subtelocentric chromosomes. A relationship between telomeric sequences and NORs, and

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confidence: 61%

“…Cynomops abrasus and Eumops auripendulus exhibit a smaller number of chromosomes, with 2n = 34 (FN = 64) and 2n = 42 (FN = 62), respectively (Warner et al, 1974;Morielle-Versute et al, 1996). Warner et al (1974) suggested that 48 could be the ancestral diploid number for this family, since 23 species and eight genera show 2n = 48.…”

mentioning

confidence: 99%

Short CommunicationNew insights into telomeric DNA sequence (TTAGGG)n location in bat chromosomes

Faria¹,

Marchesin²,

Moreira³

et al. 2009

Genet. Mol. Res.

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“…The karyotypes of E. glaucinus and C. perspicillata have many rearrangements, as compared to karyotypes considered primitive. Only some elements considered primitive are observed in the karyotype of E. glaucinus (Morielle-Versute et al, 1996), whereas in C. perspicillata no primitive karyotypes were identified (Stock, 1975). These observations and the FISH results of C. perspicillata indicate that other chromosomal mechanisms besides telomeric associations are likely to be involved where telomeric hybridization is absent.…”

Section: Discussionmentioning

confidence: 63%

In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I

Faria

Morielle‐Versute

2002

Genet. Mol. Biol.

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The purpose of this work was to verify the ability of the enzyme Alu I to cleave and/or remove satellite DNA sequences from heterochromatic regions in chromosomes of bats, by identifying the occurrence of modifications in the pattern of fluorescence in situ hybridization with telomeric DNA. The localization and fluorescence intensity of the telomeric DNA sites of the Alu-digested and undigested chromosomes of species Eumops glaucinus, Carollia perspicillata, and Platyrrhinus lineatus were analyzed. Telomeric sequences were detected at the termini of chromosomes of all three species, although, in C. perspicillata, the signals were very faint or absent in most chromosomes. This finding was interpreted as being due to a reduced number of copies of the telomeric repeat, resulting from extensive telomeric association and/or rearrangements undergone by the chromosomes of Carollia. Fluorescent signals were also observed in centromeric and pericentromeric regions in several two-arm chromosomes of E. glaucinus and C. perspicillata. In E. glaucinus and P. lineatus, some interstitial and terminal telomeric sites were observed to be in association with regions of constitutive heterochromatin and ribosomal DNA (NORs). After digestion, these telomeric sites showed a significant decrease in signal intensity, indicating that enzyme Alu I cleaves and/or removes part of the satellite DNA present in these regions. These results suggest that the telomeric sequence is a component of the heterochromatin, and that the C-band-positive regions of bat chromosomes have a different DNA composition.

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“…This variation ranges from one pair of NORs as observed in Diaemus youngii and Desmodus rotundus, up to five pairs in different species of Myotis (Volleth 1987;Morielle and Varella-Garcia, 1988;Souza and Araújo, 1990;Baker et al, 1992;Morielle-Versute et al, 1996). Moreover, the NORs occur both in terminal regions and interstitial regions of chromosome arms, and they generally correspond to the secondary constrictions.…”

Section: Nucleolus Organizer Regions and Nucleolar Behaviormentioning

confidence: 93%

Nucleolar behavior during meiosis in four species of phyllostomid bats (Chiroptera, Mammalia)

Beguelini¹,

Marchesin²,

Azeredo-Oliveira³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. We analyzed the behavior of the nucleolus, nucleolar structures and nucleolus organizer regions (NORs) during meiotic division in four species of phyllostomid bats that have different numbers and locations of NORs. Nucleoli began disassembly at leptotene, and the subcomponents released from the nucleolus were dispersed in the nucleoplasm, associated with perichromosomal regions, or they remained associated with NORs throughout division. In Phyllostomus discolor, a delay in nucleolus disassembly was observed; it disassembled by the end of pachytene. The RNA complexes identified by acridine orange staining were observed dispersed in the nucleoplasm and associated with perichromosomal regions. FISH with rDNA probe revealed the number of NORs of the species: one NOR in Carollia per- Nucleolar behavior in meiosis of bats spicillata, one pair in Platyrrhinus lineatus and P. discolor, and three pairs in Artibeus lituratus. During pachytene, there was a temporary dissociation of the homologous NORs, which returned to pairing at diplotene. The variation in the number (from one to three pairs) and location of NORs (in sex or autosomal chromosomes, at terminal or interstitial regions) did not seem to interfere with the nucleolar behavior of the different species because no variation in nucleolar behavior that could be correlated with the variation in the number and chromosomal location of NORs was detected.

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Karyotypic patterns of seven species of molossid bats (Molossidae, Chiroptera)

Cited by 30 publications

References 4 publications

Short CommunicationNew insights into telomeric DNA sequence (TTAGGG)n location in bat chromosomes

Short CommunicationNew insights into telomeric DNA sequence (TTAGGG)n location in bat chromosomes

In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I

Nucleolar behavior during meiosis in four species of phyllostomid bats (Chiroptera, Mammalia)

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