Karyotypic, palynological, and RAPD study on 12 taxa from two subsections of section Idaeobatus in Rubus L. and taxonomic treatment of R. ellipticus, R. pinfaensis, and R. ellipticus var. obcordatus

Wang, Xiaorong; Tang, Haoru; Zhang, Hanwu; BiFeng, Zhong; Xia, Wu-Feng; Liu, Yuan

doi:10.1007/s00606-009-0190-8

Cited by 7 publications

(4 citation statements)

References 13 publications

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“…Taxonomic treatments of R. ellipticus, R. ellipticus var. obcordatus , and R. pinfaensis have been fraught with controversy for a long time (Van Thuan, 1968 ; Lauener, 1970 ; Yü et al, 1985 ; Li and He, 2001 ; Lu and Boufford, 2003 ; Wang et al, 2009 ). Rubus ellipticus and R. ellipticus var.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Insights into Chinese Rubus (Rosaceae) from Multiple Chloroplast and Nuclear DNAs

Wang

Chen

et al. 2016

Front. Plant Sci.

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Rubus L. is a large and taxonomically complex genus, species of which exhibit apomixis, polyploidy, and frequent hybridization. Most of Chinese Rubus are assigned in two major sections, Idaeobatus and Malachobatus. To explore the phylogenetic relationships within Chinese Rubus, inferences upon three chloroplast DNA (rbcL, rpl20-rps12, and trnG-trnS), nuclear ribosomal ITS, and two low-copy nuclear markers (GBSSI-2 and PEPC) were deduced in 142 Rubus taxa from 17 subsections in 6 sections. nrITS and GBSSI-2 were the most informative among the six DNA regions assessed. Phylogenetic relationships within Rubus were well-resolved by combined nuclear datasets rather than chloroplast markers. The phylogenetic inferences strongly supported that section Idaeobatus was a polyphyletic group with four distant clades. All samples of sect. Malachobatus formed a monophyletic clade, in which R. tsangorum and R. amphidasys of sect. Dalibardastrum, and R. peltatus from subsection Peltati of sect. Idaeobatus were always nested. Rubus pentagonus (2n = 2x = 14) from subsect. Alpestres of sect. Idaeobatus was a sister group to the polyploid sect. Malachobatus, as well as the polytomy of three sect. Cyalctis members. This suggests that some polyploids of Malachobatus might originate from common ancestors, via polyploidization of hybrids between R. pentagonus and sect. Cylactis species. They had experienced species explosion in a short time. Section Dalibardastrum species have potential parental lineages from subsects. Moluccani and Stipulosi of sect. Malachobatus. Based on molecular phylogenies, we also provided recommendations for the taxonomic treatments of four taxa. In addition, our results showed certain incongruence between chloroplast and nuclear markers, which might be due to hybridization and introgression.

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Section: Discussionmentioning

confidence: 99%

Phylogenetic Insights into Chinese Rubus (Rosaceae) from Multiple Chloroplast and Nuclear DNAs

Wang

Chen

et al. 2016

Front. Plant Sci.

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“…In previous studies, the relationships among three taxa, R. ellipticus , R. ellipticus var. obcordatus , and R. wallichianus , were controversial [ 24 , 85 , 86 ]. Our phylogenetic analysis revealed that R. ellipticus has a more distant relationship with R. wallichianus and R. ellipticus var.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Complete Chloroplast Genomes of Rubus in China: Hypervariable Regions and Phylogenetic Relationships

Xu,

Li,

Chen

et al. 2024

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With more than 200 species of native Rubus, China is considered a center of diversity for this genus. Due to a paucity of molecular markers, the phylogenetic relationships for this genus are poorly understood. In this study, we sequenced and assembled the plastomes of 22 out of 204 Chinese Rubus species (including varieties) from three of the eight sections reported in China, i.e., the sections Chamaebatus, Idaeobatus, and Malachobatus. Plastomes were annotated and comparatively analyzed with the inclusion of two published plastomes. The plastomes of all 24 Rubus species were composed of a large single-copy region (LSC), a small single-copy region (SSC), and a pair of inverted repeat regions (IRs), and ranged in length from 155,464 to 156,506 bp. We identified 112 unique genes, including 79 protein-coding genes, 29 transfer RNAs, and four ribosomal RNAs. With highly consistent gene order, these Rubus plastomes showed strong collinearity, and no significant changes in IR boundaries were noted. Nine divergent hotspots were identified based on nucleotide polymorphism analysis: trnH-psbA, trnK-rps16, rps16-trnQ-psbK, petN-psbM, trnT-trnL, petA-psbJ, rpl16 intron, ndhF-trnL, and ycf1. Based on whole plastome sequences, we obtained a clearer phylogenetic understanding of these Rubus species. All sampled Rubus species formed a monophyletic group; however, sections Idaeobatus and Malachobatus were polyphyletic. These data and analyses demonstrate the phylogenetic utility of plastomes for systematic research within Rubus.

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“…On the contrary, the differences between R. ellipticus and R. ellipticus var. obcordatus not only focus on the leaflet shape and size, but also on the growth habits and habitat, inflorescence and flowering time [39]. Moreover, significant differences also exhibited in the pollen features, rDNA chromosomal distribution and genomic relationships by molecular cytogenetics [12, 39, 40].…”

Section: Discussionmentioning

confidence: 99%

Allopolyploid origin in Rubus (Rosaceae) inferred from nuclear granule-bound starch synthase I (GBSSI) sequences

Wang

Chen

et al. 2019

BMC Plant Biol

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Background Polyploidy and hybridization are ubiquitous in Rubus L., a large and taxonomically challenging genus. Chinese Rubus are mainly concentrated into two major sections, the diploid Idaeobatus and the polyploid Malachobatus . However, it remains unclear to be auto- or allo- polyploid origin of polyploids in Rubus . We investigated the homoeologs and the structure of the GBSS I-1 (granule-bound starch synthase I) gene in 140 Rubus individuals representing 102 taxa in 17 (out of the total 24) subsections of 7 (total of 12) sections at different ploidy levels. Results Based on the gene structure and sequence divergence, we defined three gene variants, GBSS I-1a, GBSS I-1b, and GBSS I-1c. When compared with GBSS I-1a, both GBSS I-1b and GBSS I-1c have a shorter fourth intron, and GBSS I-1c had an additional deletion in the fifth intron. For diploids, either GBSS I-1a or GBSS I-1b was detected in 56 taxa consisting of 82 individuals from sect. Idaeobatus , while both alleles existed in R. pentagonus and R. peltatus . Both homoeologs GBSS I-1a and GBSS I-1b were identified in 39 taxa (48 individuals) of Malachobatus polyploids. They were also observed in two sect. Dalibardastrum taxa, in one sect. Chamaebatus taxon, and in three taxa from sect. Cylactis . Interestingly, all three homoeologs were observed in the three tetraploid taxa. Phylogenetic trees and networks suggested two clades (I and II), corresponding to GBSS I-1a, and GBSS I-1b/1c sequences, respectively. GBSS I-1 homoeologs from the same polyploid individual were resolved in different well-supported clades, and some of these homoelogs were more closely related to homoelogs in other species than they were to each other. This implied that the homoeologs of these polyploids were donated by different ancestral taxa, indicating their allopolyploid origin. Two kinds of diploids hybridized to form most allotetraploid species. The early-divergent diploid species with GBSS I-1a or -1b emerged before polyploid formation in the evolutionary history of Rubus . Conclusion This study provided new insights into allopolyploid origin and evolution from diploid to polyploid within the genus ...

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Karyotypic, palynological, and RAPD study on 12 taxa from two subsections of section Idaeobatus in Rubus L. and taxonomic treatment of R. ellipticus, R. pinfaensis, and R. ellipticus var. obcordatus

Cited by 7 publications

References 13 publications

Phylogenetic Insights into Chinese Rubus (Rosaceae) from Multiple Chloroplast and Nuclear DNAs

Phylogenetic Insights into Chinese Rubus (Rosaceae) from Multiple Chloroplast and Nuclear DNAs

Comparative Analysis of Complete Chloroplast Genomes of Rubus in China: Hypervariable Regions and Phylogenetic Relationships

Allopolyploid origin in Rubus (Rosaceae) inferred from nuclear granule-bound starch synthase I (GBSSI) sequences

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