Karyotypic and molecular cytogenetic characterization of diploid and polyploid accessions of medicinal herbs in the genus Paris from northern Thailand

Chow, Janene; Puangpairote, Tidarat; Anamthawat‐Jónsson, Kesara; Umpunjun, Puangpaka

doi:10.2306/scienceasia1513-1874.2020.037

Cited by 3 publications

(1 citation statement)

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“…Since pretreatment is necessary (Rezaei et al, 2014), so the 2 cm-long roots were first cold pretreated for 1.5 h at 4 ºC, followed by chemical pretreatment in 0.05 M aqueous colchicine solution for 2.5 h (Ahmadi-Roshan et al, 2016). They were then fixed in acetic acid: alcohol (1:3) for 1 h. The roots were hydrolyzed for 15 min in 1M HCl, followed by staining with aceto-orcein 2% (w/v) for 2 h (Chowa et al, 2020;Sayadi et al, 2021;Najafi et al, 2022). Squash method was applied at 45% HCl (v/v) for preparing slides.…”

Section: Cytological Preparationmentioning

confidence: 99%

Chromosomal and genome size variations in Opium poppy (Papaver somniferum L.) from Afghanistan

Rasekh,

Karimzadeh

2024

Caryologia

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The genus Papaver classified in the Papaveraceae family, is a valuable, non-alternative medicinal plant which has illustrated a massive variety of pharmacologically important alkaloids. Chromosomal and monoploid genome size diversity of seven populations collected from different districts of Balkh Province in northern parts of Afghanistan were studied. All populations were diploid, six of which (P1-P6) had 22 chromosomes, while P7 had 20 larger chromosomes. The mean chromosome length (CL) of P1-P6 populations was 1.32 μm (0.91-1.74 μm), but that of P7 population was 2.24 μm. The results of flow cytometric analysis showed that the mean monoploid 2Cx DNA of P1-P6 populations was 5.701 pg (5.574-5.901 pg), whereas that of P7 population was 5.795 pg, confirming intraspecific variation. This study is being reported for the first time from the northern part of Afghanistan’s opium cultivation area, and P7 population is also being reported for the first time in terms of chromosome number. Valuable information on Cytogenetics can be used in some research fields, including polygenetic analysis, taxonomic relationships, evolutionary characteristics, and plant breeding.

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Section: Cytological Preparationmentioning

confidence: 99%

Chromosomal and genome size variations in Opium poppy (Papaver somniferum L.) from Afghanistan

Rasekh,

Karimzadeh

2024

Caryologia

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The size of viable pollen is correlated with ploidy level, as evidenced by a polyploid series of <i>Ocimum</i> L. from Thailand

Lekhapan,

Anamthawat-Jónsson,

Chokchaichamnankit

2024

cytologia

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Revision on the Genus Paris in Thailand, with a New Species Paris siamensis

Ruchisansakun

Sraphet

Yothawut³

et al. 2023

Plants

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The genus Paris is an important and confusing taxon due to high variation within species, and differences between species are sometimes difficult to delimit. Thus, the status of some taxa has changed over time. To clarify the status of Paris species for plant conservation and effective management of this genus in Thailand, we performed an intensive survey in northern Thailand, studied morphological characteristics, and constructed a molecular phylogenic tree, which we compared to recently published results of this genus. Our results indicate that there are two species in Thailand: P. yunnanensis and a new species, P. siamensis. Detailed descriptions, illustrations, and the phylogenetic position of these two species are provided here.

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Karyotypic and molecular cytogenetic characterization of diploid and polyploid accessions of medicinal herbs in the genus Paris from northern Thailand

Cited by 3 publications

References 0 publications

Chromosomal and genome size variations in Opium poppy (Papaver somniferum L.) from Afghanistan

Chromosomal and genome size variations in Opium poppy (Papaver somniferum L.) from Afghanistan

The size of viable pollen is correlated with ploidy level, as evidenced by a polyploid series of <i>Ocimum</i> L. from Thailand

Revision on the Genus Paris in Thailand, with a New Species Paris siamensis

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