Karyotype reshufflings of Festuca pratensis × Lolium perenne hybrids

Majka, Joanna; Zwierzykowski, Z.; Majka, Maciej; Kosmala, Arkadiusz

doi:10.1007/s00709-017-1161-5

Cited by 10 publications

(8 citation statements)

References 45 publications

(62 reference statements)

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“…Human fragile sites are known as hotspots for chromosome aberrations, especially in many tumor cells [37, 38]. However, in Festuca × Lolium plants, the analysis of the relation between FSs associated with 35S rDNA regions and the location of structural rearrangements revealed that intergenomic changes did not correspond to FSs [39].…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic and molecular genotyping in the allotetraploid Festuca pratensis × Lolium perenne hybrids

et al. 2019

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Background Species of the Festuca and Lolium genera, as well as intergeneric Festuca × Lolium ( Festulolium ) hybrids, are valuable fodder and turf grasses for agricultural and amenity purposes worldwide. Festulolium hybrids can merge in their genomes agronomically important characteristics. However, in polyploid plants, especially in allopolyploids, the hybridization of divergent genomes could contribute to various abnormalities, such as variability in chromosome number, structural rearrangements, and/or disorders in inheritance patterns. Here we studied these issues in allotetraploid Festuca pratensis × Lolium perenne hybrids. Results Cytogenetic procedures, including fluorescent in situ hybridization, genomic in situ hybridization, and molecular markers – inter-simple sequence repeats (ISSR) were exploited. This cytogenetic approach indicated the dynamics in the number and distribution of ribosomal RNA genes and structural rearrangements for both parental genomes ( Festuca and Lolium ) in hybrid karyotypes. The separate analysis of F. pratensis and L. perenne chromosomes in hybrid plants (F 2 -F 3 generations of F. pratensis × L. perenne ) revealed the asymmetrical level of rearrangements. Recognized structural changes were mainly located in the distal part of chromosome arms, and in chromosomes bearing ribosomal DNA, they were more frequently mapped in arms without this sequence. Based on the ISSR markers distribution, we found that the tetrasomic type of inheritance was characteristic for the majority of ISSR loci, but the disomic type was also observed. Nonetheless, no preference in the transmission of either Festuca or Lolium alleles to the following generations of allotetraploid F. pratensis × L. perenne hybrid was observed. Conclusion Our study reports cytogenetic and molecular genotyping of the F. pratensis × L. perenne hybrid and its following F 2 -F 3 progenies. The analysis of 137 allotetraploid F. pratensis × L. perenne hybrids revealed the higher level of recombination in chromosomes derived from F. pratensis genome. The results of ISSR markers indicated a mixed model of inheritance, which may be characteristic for these hybrids. Electronic supplementary material The online versio...

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Section: Discussionmentioning

confidence: 99%

Cytogenetic and molecular genotyping in the allotetraploid Festuca pratensis × Lolium perenne hybrids

et al. 2019

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“…This technique allows for the genome-specific chromosome labeling in the cells of allopolyploid or hybrid species. It has been widely used in plant studies to identify putative parental genomes in the interspecific or intergeneric hybrids or allopolyploids (for the review see Chester et al, 2010 ; Silva & Souza, 2013 ), to study the evolution of polyploid genomes ( Song et al, 1995 ; Gaeta et al, 2007 ; Majka et al, 2018 ; Tan et al, 2017 ), or to detect the introgression of alien chromatin ( Schneider et al, 2005 ; Tan et al, 2005 ). It has also become a valuable tool to analyze the meiotic recombination and behavior of chromosomes in the hybrids ( Zwierzykowski et al, 2008 ) as well as translocation ( Kruppa et al, 2013 ), addition ( Molnar-Lang et al, 2000 ; Ji & Chetelat, 2007 ), or substitution ( Pan et al, 2017 ) lines.…”

Section: Discussionmentioning

confidence: 99%

Complex characterization of oat (Avena sativaL.) lines obtained by wide crossing with maize (Zea maysL.)

Skrzypek

Warzecha

Noga

et al. 2018

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BackgroundThe oat × maize addition (OMA) lines are used for mapping of the maize genome, the studies of centromere-specific histone (CENH3), gene expression, meiotic chromosome behavior and also for introducing maize C4 photosynthetic system to oat. The aim of our study was the identification and molecular-cytogenetic characterization of oat × maize hybrids.MethodsOat DH lines and oat × maize hybrids were obtained using the wide crossing of Avena sativa L. with Zea mays L. The plants identified as having a Grande-1 retrotransposon fragment, which produced seeds, were used for genomic in situ hybridization (GISH).ResultsA total of 138 oat lines obtained by crossing of 2,314 oat plants from 80 genotypes with maize cv. Waza were tested for the presence of maize chromosomes. The presence of maize chromatin was indicated in 66 lines by amplification of the PCR product (500 bp) generated using primers specific for the maize retrotransposon Grande-1. Genomic in situ hybridization (GISH) detected whole maize chromosomes in eight lines (40%). All of the analyzed plants possessed full complement of oat chromosomes. The number of maize chromosomes differed between the OMA lines. Four OMA lines possessed two maize chromosomes similar in size, three OMA—one maize chromosome, and one OMA—four maize chromosomes. In most of the lines, the detected chromosomes were labeled uniformly. The presence of six 45S rDNA loci was detected in oat chromosomes, but none of the added maize chromosomes in any of the lines carried 45S rDNA locus. Twenty of the analyzed lines did not possess whole maize chromosomes, but the introgression of maize chromatin in the oat chromosomes. Five of 66 hybrids were shorter in height, grassy type without panicles. Twenty-seven OMA lines were fertile and produced seeds ranging in number from 1–102 (in total 613). Sixty-three fertile DH lines, out of 72 which did not have an addition of maize chromosomes or chromatin, produced seeds in the range of 1–343 (in total 3,758). Obtained DH and OMA lines were fertile and produced seeds.DiscussionIn wide hybridization of oat with maize, the complete or incomplete chromosomes elimination of maize occur. Hybrids of oat and maize had a complete set of oat chromosomes without maize chromosomes, and a complete set of oat chromosomes with one to four retained maize chromosomes.

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“…Another issue affecting the extent of use of Festulolium in the grass business is the stability of hybrid genomes and the potential for the elimination of chromatin of one of the species in consecutive generations of sexual reproduction. It has been observed that the Lolium chromatin prevails in the Festulolium hybrids (Zwierzykowski et al 1998, Kopecký et al 2006, Książczyk et al 2015, Majka et al 2018a. Zwierzykowski et al (2006Zwierzykowski et al ( , 2011 observed slow and gradual replacement of the Festuca chromosomes by those of Lolium in consecutive generations of F. pratensis × L. perenne hybrids (Fig.…”

Section: Genome Balance and Stability Of Hybridsmentioning

confidence: 95%

Cytogenetic insights into Festulolium

Majka¹,

Majka²,

Kopecký³

et al. 2020

Biol. plant.

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Climate change calls for new methods and plant materials to breed crops adapted to new environmental conditions. Sustainable forage and amenity grass production during periods of severe drought and heat waves during summer, and unequal distribution of precipitation over the year will require drought-tolerant genotypes. However, high-yielding ryegrasses (Lolium spp.), which are the most commonly used grass species, suffer during abiotic stresses. Introgression of drought and heat tolerance from closely related fescues (Festuca spp.) offers an opportunity to develop superior hybrid cultivars to mitigate the negative impact of climate change. Intergeneric cross-hybridization and the development of Festulolium (Festuca × Lolium) hybrids was initiated 100 years ago and resulted in registration of almost one hundred cultivars. For a long time, their genome composition was not known and was debated by breeders and geneticists. In the last three decades, molecular cytogenetic and genomic approaches have enabled their detailed characterization. These studies revealed a gradual replacement of Festuca chromosomes by those of Lolium in consecutive generations leading to an almost complete elimination of Festuca chromatin in the introgression forms. On the other hand, amphiploid cultivars seem to be more stable with the optimal proportions of the Lolium to Festuca genomes at about 2:1. In this mini review, we discuss recent advances in the analysis of the genome composition of Festulolium hybrids with a specific focus on genome (in)stability.

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Karyotype reshufflings of Festuca pratensis × Lolium perenne hybrids

Cited by 10 publications

References 45 publications

Cytogenetic and molecular genotyping in the allotetraploid Festuca pratensis × Lolium perenne hybrids

Cytogenetic and molecular genotyping in the allotetraploid Festuca pratensis × Lolium perenne hybrids

Complex characterization of oat (Avena sativaL.) lines obtained by wide crossing with maize (Zea maysL.)

Cytogenetic insights into Festulolium

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