Karyotype of Slash Pine (Pinus elliottii var. elliottii) Using Patterns of Fluorescence in situ Hybridization and Fluorochrome Banding

Doudrick, R. L.; Heslop‐Harrison, J.; Nelson, C. Dana; Schmidt, Thomas; Nance, Warren L.; Schwarzacher, Trude

doi:10.1093/oxfordjournals.jhered.a111583

Cited by 82 publications

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“…Data on banding patterns can be used for comparative karyotyping in some species, and they detect inter-and intraspecific variation patterns (Hizume et al 1983(Hizume et al , 1989(Hizume et al , 1990; however, fluorescent banding does not provide sufficient information for the discrimination of all homologous chromosome pairs. Not all homologous pairs in Pinus species were identified by probe signals in studies of karyotypes by FISH that used 35S rDNA and 5S rDNA probes (Doudrick et al 1995, Liu et al 2003, Cai et al 2006, Bogunić et al 2011, telomere sequences (Fuchs et al 1995, Shibata et al 2005, Islam-Faridi et al 2007, or simple sequence repeats (SSR) (Pavia et al 2014). Combined fluorescent banding and FISH investigations have identified interstitial and proximal CMA bands that are consistent with 35S rDNA and/or the short repetitive sequence proximal CMA band-specific repeat (PCSR) loci, and interstitial DAPI bands that are consistent with signals of repetitive sequences containing the Arabidopsis-type telomere sequence (Hizume et al 2001, Shibata et al 2005.…”

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A Comparative Analysis of Multi-Probe Fluorescence In Situ Hybridisation (FISH) Karyotypes in 26 Pinus Species (Pinaceae)

2016

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Summary Every species has a unique karyotype, but certain genera have common karyptypes among species. The markers (chromosome length and centromere position) used in traditional karyotyping do not distinguish all chromosome pairs in the genus Pinus. However, the application of multi-probe fluorescence in situ hybridisation (FISH) procedures allowed exact karyotyping of 26 Pinus congeners. We used these new data to examine species relationships. The 35S rDNA and 5S rDNA, Arabidopsis-type telomere repeat sequences, and the proximal CMA band-specific repeat (PCSR) of P. densiflora were used as FISH probes for our analysis of chromosomes in 26 Pinus species. Each species had a unique FISH karyotype and most homologous chromosome pairs were identified. The FISH karyotypes were used to compare corresponding or homologous chromosomes among the species. Common or similar FISH signal patterns appeared in closely related species. Species that had inherited common FISH signal patterns were classified into four karyotype groups. We used cluster analyses to compare quantitative differences in FISH signals within these groups. The results of these analyses were consistent with recent systematic interpretations and resolved differences among existing taxonomic systems based on diverse methodologies. Our results indicate that FISH signal patterns reflect the history of species differentiation and that comparative FISH karyotyping has potential as an important tool for studying the taxonomy or phylogeny of Pinus.

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confidence: 99%

A Comparative Analysis of Multi-Probe Fluorescence In Situ Hybridisation (FISH) Karyotypes in 26 Pinus Species (Pinaceae)

2016

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“…For more detail understands of chromosomes excellent techniques need to be used. In Pinus several techniques have been applied to chromosome analyses such as observation of small constrictions, C-banding, G-banding, fluorescent banding and in situ hybridization (Tanaka and Hizume 1980;Hizume et al 1983Hizume et al , 1989Hizume et al , 1990Hizume et al , 2002Doudrick et al 1995;Lubaretz et al 1996;Jacobs et al 2000;Shibata et al 2016;Bogunić et al 2011a,b). An effectiveness of fluorescent banding method and fluorescent in situ hybridization (FISH) using several proves is demonstrated in several pine species (Hizume et al 1983(Hizume et al , 1989(Hizume et al , 1990(Hizume et al , 2002Bogunić et al 2011a, b).…”

Section: Abstract: Chromosome Diploxylon Pine Fluorescent Band Karmentioning

confidence: 99%

Chromosome banding in the genus Pinus V. Fluorescent banding patterns in 16 diploxylon pines

et al. 2016

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ABSTRACT:In Pinus , Subgenus Pinus 16 species were investigated on their somatic chromosomes by a fluorescent banding technique using chromomycin A 3 (CMA) and 4',6-diamidino-2-phenylindole (DAPI). The chromosome number of 2n=24 was commonly counted in all the species studied. Their karyotypes were composed of many long metacentric chromosomes and a few short submetacentric chromosomes, and two karyotypes were recognized in respect to number of short chromosomes in the subgenus. CMA-bands appeared at an interstitial and/or proximal region of chromosomes and DAPI-band did at a proximal region. Thin DAPI-bands appeared at interstitial regions and DAPI-dots appeared at a centromeric region in most chromosomes. In a chromosome complement each homologous chromosome was identified on the base of its shape and fluorescent banding pattern. The typical banding patterns were compared among the species studied. Many interstitial CMA-bands at the secondary constrictions appeared on many metacentric chromosomes. Proximal fluorescent bands varied in fluorescent nature and number and divided into several groups. Section Pinus short three chromosome pairs were two or three patterns of fluorescent banding. The section Trifolae species had many proximal DAPI-bands and less proximal CMA-bands than section Pinus. The shortest chromosome had proximal bands indicating two groups on the kinds of fluorescent band.

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“…1-3, 5, 6;Shibata et al 2004, Puizina et al 2008. Several species of Pinus (Hizume et al 1983, 1990, Doudrick et al 1995 and of Picea Carlson 1997, Siljak-Yakovlev et al 2002) also have complex DAPIbanding patterns; these variations in DAPI-banding pattern among species might occur independently in each genus and indicate that DAPI-banding patterns should be evaluated within the genus. The phenomenon might be coincident with taxonomic and phylogenetic treatment in each genus.…”

Section: Abies Georgeimentioning

confidence: 99%

Fluorescent Chromosome Banding Patterns in Six Species of Abies, Pinaceae

2016

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SummaryThe six Abies species, A. laciocarpa, A. veitchii, A. sachalinensis, A. mariesii, A. faxoniana and A. georgei, were investigated for their chromosomes by the fluorescent banding method using fluorochromes chromomycin A 3 (CMA) and 4′,6-diamidino-2-phenylindole (DAPI). All six species have 2n=24 chromosomes and a similar karyotype consisting of seven pairs of long metacentric chromosomes and five pairs of shorter submetaand subtelocentric chromosomes, supporting previous studies. Eight clear CMA-bands appeared at the interstitial region of one arm of long metacentric chromosomes in all species, and in A. veitchii and A. sachalinensis, their number varied from six to eight among plants and/or populations. A weak CMA-band appeared at the interstitial region of one chromosome pair, while a weak CMA-band appeared at the proximal region in some species. In most species DAPI did not produce clear bands, and sites of clear CMA-bands were DAPI negative. Only A. mariesii showed many DAPI-bands at the interstitial and/or centromeric regions of several chromosomes. A few weak DAPI-bands appeared in some other species. The fluorescent banding and FISH patterns reported in Abies species were compared and discussed with taxonomic treatment and molecular phylogeny of Abies.

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Karyotype of Slash Pine (Pinus elliottii var. elliottii) Using Patterns of Fluorescence in situ Hybridization and Fluorochrome Banding

Cited by 82 publications

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A Comparative Analysis of Multi-Probe Fluorescence <i>In Situ</i> Hybridisation (FISH) Karyotypes in 26 <i>Pinus</i> Species (Pinaceae)

A Comparative Analysis of Multi-Probe Fluorescence <i>In Situ</i> Hybridisation (FISH) Karyotypes in 26 <i>Pinus</i> Species (Pinaceae)

Chromosome banding in the genus <i>Pinus</i> V. Fluorescent banding patterns in 16 diploxylon pines

Fluorescent Chromosome Banding Patterns in Six Species of <i>Abies</i>, Pinaceae

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