Karyotype diversity in cultivated and wild Indian rice through EMA-based chromosome analysis

Jha, Timir Baran

doi:10.1007/s12041-021-01332-z

Cited by 4 publications

(3 citation statements)

References 52 publications

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“…Previously, DAPI +ve signals on centromeric and intercalary regions and CMA +ve /DAPI -ve signals on nucleolar regions are reported in the phylogenetically close A. brac-teata (Pedrosa et al 2001). Therefore, distribution of nonnucleolar AT-rich heterochromatin (depicted by DAPI +ve bands, Bhowmick and Jha 2015, 2019, 2021 may follow differential distribution among the species of subgenus Urophyllon, to be confirmed after analysis of DAPI banding pattern in A. volubilis and related species in question (Goldblatt and Manning 2011). Till then, DAPI -ve NORs remain to be a conserved chromosomal landmark so far in the subgen.…”

Section: Confirmation Of Nucleolar Chromosomes Following Dapi Stainingmentioning

confidence: 90%

“…Hence the aim of the present work is to obtain better cytogenetic analysis with enzymatic maceration and air drying (EMA) method (Kurata and Omura 1978;Fukui 1996) in A. virens over the traditional orcein staining approach. In spite of being a well-known alternate method for chromosome analysis (Fukui and Iijima 1991;Yamamoto et al 2010Yamamoto et al , 2015Nath et al 2015;Jha et al 2020;Jha 2021;Bhowmick and Jha 2022), EMA based approach has not been yet attempted in Albuca virens. Our adoption of EMA method enabled lucid karyotype interpretation, fluorescence staining and understanding meiotic chromosome behavior in this plant.…”

Section: Introductionmentioning

confidence: 99%

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Unfolding chromosomal uniqueness of the Scilloid ornamental Albuca virens by application of EMA based Giemsa- DAPI staining

Bhowmick,

Nag

2023

Caryologia

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The cytogenetic features in the tribe Ornithogaleae of subfamily Scilloideae is a prerequisite for understanding genome evolution. Unfortunately, genomic or the foundational chromosomal features are neglected within majority of Ornithogaloids, incuding Albuca, one of the largest genus of the tribe. Albuca virens (Lindl.) J.C. Manning & Goldblatt is the only ornamental species of Albuca found to be exotic to India. Analysis of karyotype by EMA method followed by Giemsa and DAPI staining is the first step towards molecular cytogenetics attempted in this species and has currently brought significant resolution in chromosome morphology (especially NORs). The Indian population of A. virens with 2n=6 chromosomes, symmetric karyotype and heterophorphy of NORs provide excellent scope to navigate questions on dysploid origin of Albuca. The regular meiotic stages advocate genomic stability despite vegetative propagation and polysomaty in root cells. The comparative review of chromosomal evolution within Albuca has been discussed in relation to the Indian A. virens as a prototype.

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Section: Confirmation Of Nucleolar Chromosomes Following Dapi Stainingmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Unfolding chromosomal uniqueness of the Scilloid ornamental Albuca virens by application of EMA based Giemsa- DAPI staining

Bhowmick,

Nag

2023

Caryologia

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“…Over the years, molecular cytogenetics has rejuvenated research on plant chromosomes. Chromosomes prepared through the enzymatic maceration and air drying (EMA) method followed by Giemsa staining [34,35] provides distinct chromosomal morphology in a cytoplasm-free background. Application of contrasting base-specific fluorochrome dyes 4 -6-Diamidino-2-phenylindole (DAPI) and Chromomycin A 3 (CMA) helps to identify heterochromatin blocks of repetitive DNA sequences directly on the chromosomes [36].…”

Section: Introductionmentioning

confidence: 99%

Comparative Cytogenetics and Fluorescent Chromosome Banding in Five Indian Species of Dipcadi Medik

Samanta

Jha²,

Ray

et al. 2023

Plants

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The genus Dipcadi Medik. (Subfamily: Scilloideae) has a narrow distribution in India and several overlapping morphological traits make the genus taxonomically challenging at the species level. Cytogenetic characterization can provide additional taxonomic data and can be used to evaluate genetic diversity at the species level. We have accomplished comparative karyotype analysis and fluorescence banding patterns using 4′-6-Diamidino-2-phenylindole (DAPI) and Chromomycin A3 (CMA) in five Indian species for the first time. The karyotypes of D. concanense and D. goaense exhibited similar fluorochrome banding profiles. However, D. montanum, D. ursulae and D. erythraeum differ distinctly in their karyotypes. In all taxa, CMA+ve/DAPI−ve or DAPI0 (GC-rich) constitutive heterochromatin was located at the constriction region or terminal satellite of the nucleolar chromosome. DAPI+ve/CMA−ve or CMA0 (AT-rich) heterochromatin dominates in D. montanum, D. ursulae and D. erythraeum. However, D. erythraeum shows a distinct variation in fluorochrome banding pattern from all other species. The distribution of CMA and DAPI bands is a reflection of heterochromatin composition and variations acquired by different species. This characterization can be used to assess phylogenetic relationships in the understudied genus Dipcadi and may serve as a basis for other genomic analyses and evolutionary studies.

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Evaluation of karyotype diversity in Indian traditional aromatic rice cultivars through EMA-based non-fluorescent Giemsa and fluorescent DAPI staining

Jha,

Halder

2023

Genet Resour Crop Evol

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Karyotype diversity in cultivated and wild Indian rice through EMA-based chromosome analysis

Cited by 4 publications

References 52 publications

Unfolding chromosomal uniqueness of the Scilloid ornamental Albuca virens by application of EMA based Giemsa- DAPI staining

Unfolding chromosomal uniqueness of the Scilloid ornamental Albuca virens by application of EMA based Giemsa- DAPI staining

Comparative Cytogenetics and Fluorescent Chromosome Banding in Five Indian Species of Dipcadi Medik

Evaluation of karyotype diversity in Indian traditional aromatic rice cultivars through EMA-based non-fluorescent Giemsa and fluorescent DAPI staining

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