Karyotype comparison between<i>Haliotis rubra</i>,<i>H. laevigata</i>and the interspecific hybrid

Botwright, Natasha A.

doi:10.1111/are.12156

Cited by 9 publications

(8 citation statements)

References 29 publications

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“…0.05% for 3h) is similar to the findings for optimization in post-larval stages of some other fish species (e.g. Liu et al, 2001;Botwright 2015;Zhao et al, 2015;Huang et al, 2017). This is, however, at variance with the finding of Shao et al, (2010) for eggs and larvae of Japanese flounder (Paralichthys olivaceus) and summer flounder (Paralichthys dentatus) as they reported best metaphase chromosomes with 0.02% for 1-2hours.…”

Section: Discussionsupporting

confidence: 85%

“…Despite the ease of chromosome preparation from eggs and larvae of fish (Shao et al, 2010, Karami et al, 2010Amar-Basulto et al, 2011;Botwright 2015), cytogenetic studies involving specific tissues of fingerlings, juvenile or adult fish may have some merits over the use of whole larvae or egg. Aside the possibility of having a large amount of tissue from which metaphase chromosome can be isolated multiple times; this method has a pride of place in the characterization of the progenies of intergeneric hybridization (distance crosses) between different fish species from different genus (Okomoda et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Peer Review #1 of "Optimization of the cytogenetic protocol for Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822) (v0.1)"

Thomas¹

2018

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To obtain well spread chromosomes, the cytogenetic protocol for Pangasianodon hypophthalmus and Clarias gariepinus were optimized. This includes, the colchicine concentration (0.01, 0.025, 0.05 %) / exposure duration (1, 3, and 5 h), hypotonic solution (distilled water or 0.075M KCl solution) /exposure duration (30min, 1h, and 2h), the time of cell suspension preparation (at hypotonic treatment or before slide preparation) and chromosome aging period (0, 3, and 7 days in Carnoy's fixative). In addition, the type (i.e. fin, gill or kidney) and the amount of tissue (10, 50, 100 or 150mg) were also investigated. Regardless of the species, the result obtained showed that well-spread chromosomes could be obtained using the following optimized protocol: Juveniles are injected with 0.05% colchicine (at 1mlkg-1) and allowed to swim for 3h. Then, 50mg of gill tissue is made into cell suspension in 0.075M KCl for 1h. The cell suspension is treated in Carnoy's fixative (changed 3times at 20min interval) and then aged for three days. Finally, chromosome slides are made and stained with 10% Giemsa for 1h.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "Optimization of the cytogenetic protocol for Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822) (v0.1)"

Thomas¹

2018

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“…Distant crossing has been used to increase the possibility of variation in hybrid offspring (Barton, 2001;Gerber, Tibbets, & Dowling, 2001;Litsios & Salamin, 2014;Mallet, 2007). This strategy makes it possible for the total haploid genome of one species to combine with that of another.…”

Section: Discussionmentioning

confidence: 99%

“…Generally, the desired goal is to obtain offspring that perform better than both parental species in terms of the survival rates, growth rates, flesh quality, fertilityand stress resistance. Previous reports found that better growth rates were acquired in many fish species, such as tilapia, salmonid, carp, oyster, catfish and abalone by interspecific hybridization (Al‐Harbi, ; Botwright, ; Hulata, ; Lafarga‐De, Nunez‐Acuna, & Gallardo‐Escarate, ; Refstie, ; You, Ke, Luo, & Wang, ). Crosses between different species also resulted in improved environmental and disease tolerances (Dorson, Chevassus, & Torhy, ; Lim, Leamaster, & Brock, ).…”

Section: Introductionmentioning

confidence: 99%

The obvious heterosis and genetic characters of intergeneric cross and backcross juveniles between blunt snout bream ( Megalobrama amblycephala ) and topmouth culter ( Culter alburnus )

Zheng

Guo

et al. 2019

Aquac Res

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In this study, we used artificial insemination to generate hybrid groups of fish [MC‐F1(MA♀×CA♂) and MC‐F2(MC‐F1♀×♂)] by intergeneric crosses of Megalobrama amblycephala (MA) and Culter alburnus (CA); sequential backcrosses [CAM‐B1 (CA♀×MC‐F1♂) and MCC‐B1 (MC‐F1♀×CA♂)] were also performed. All these hybrids showed high rates of fertilization, hatching and survival (p > 0.05). For genetic traits, compared with those of the M. amblycephala and C. alburnus parental lines (Table 1), the fertilization rate, hatching rate and 7‐day survival rate of MC‐F1(MA♀×CA♂), MC‐F2(MC‐F1♀×♂), CAM‐B1 (CA♀×MC‐F1♂) and MCC‐B1 (MC‐F1♀×CA♂) by artificial insemination exhibited similar high rates (p > 0.05). The morphology of the four hybrids MC‐F1/F2, CAM‐B1 and MCC‐B1 were intermediate between those of their parents. Compared with their parents of MA and CA, weight gain rate (WG), specific growth rate (SGR) and protein efficiency ratio (PER) of hybrids MC‐F1/F2, CAM‐B1 and MCC‐B1 were significantly (p < 0.05) increased and feed conversion ratio (FCR) was significantly (p < 0.05) decreased after 3 months feeding. Moreover, protein content of muscle for MC‐F1/F2, CAM‐B1 and MCC‐B1 was significantly (p < 0.05) higher and carbohydrate content of muscle was significantly (p < 0.05) lower than their parents. The females and males of the four hybrids had normal gonadal development. In this study, we successfully generated intergeneric and backcross hybridization lines with fertile potential among fish of the Cultrinae subfamily and these hybrids had obvious heterosis in terms of growth performance, feed utilization and muscle quality.

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“…Ke and You () conducted crossbreeding of a Taiwanese population ( ♀ ) × a Japanese population (♂) of Haliotis diversicolor aquatili and obtained a new strain that had significantly improved disease resistance and productivity. This was then followed by studies on abalones using crossbreeding of different geographic populations, H. diversicolor (You et al ) and H. discus hannai (Deng et al ), as well as interspecies hybridization, Haliotis laevigata × Hrubra rubra (Dang et al ; Botwright ), H. discus hannai × Haliotis gigantea (Luo et al ; Liang et al ), Haliotis rufescens × H. discus hannai (Lafarga‐de la et al , ), and H. discus hannai × Haliotis fulgens (You et al ). Genetic breeding of gastropods has also been performed in Physa acuta (Henry et al ), Melanoides tuberculata (Facon et al ), Lymnaea stagnalis (Coutellec and Caquet ), and Cornu aspersum (Nespolo et al ).…”

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confidence: 99%

Evaluation of Crosses Between Two Geographic Populations of Native Chinese and Introduced Thai Spotted Ivory Shell, Babylonia areolata, in Southern China

Lü

et al. 2016

J World Aquaculture Soc

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In the present study, intraspecific crossing experiments between Chinese (H) and Thai populations (T) of Babylonia areolata were conducted by using a complete diallel design (H, T, TH, and HT). A total of 45 pedigreed families were obtained and cultured under the same environmental conditions. Positive heterosis was observed for many developmental parameters and shell sizes for most stages from juvenile to adult. Hatching and metamorphosis occurred significantly earlier in the hybrid (HT) than in the parent groups and a crossed group (TH). Rate of metamorphosis was also higher in HT than in the other three groups. Heterosis in shell length and body weight of the crossed group (HT) from the nursery and grow-out stage to harvest time was high and significantly different from the H, T, and TH groups. For mortality rate from juvenile to adult, total and average heterosis of the crossed group (TH) was positive and significantly different from H, T, and HT. The results of the present study show that intraspecies crosses can be a potentially useful way to increase the economic traits for B. areolata and thus provide a promising method for genetic improvement of the species as a new aquaculture stock in southern China.

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Karyotype comparison betweenHaliotis rubra,H. laevigataand the interspecific hybrid

Cited by 9 publications

References 29 publications

Peer Review #1 of "Optimization of the cytogenetic protocol for Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822) (v0.1)"

Peer Review #1 of "Optimization of the cytogenetic protocol for Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822) (v0.1)"

The obvious heterosis and genetic characters of intergeneric cross and backcross juveniles between blunt snout bream ( Megalobrama amblycephala ) and topmouth culter ( Culter alburnus )

Evaluation of Crosses Between Two Geographic Populations of Native Chinese and Introduced Thai Spotted Ivory Shell, Babylonia areolata, in Southern China

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