Karyotype characterization and evolution in South American species of Lathyrus (Notolathyrus, Leguminosae) evidenced by heterochromatin and rDNA mapping

Chalup, Laura; Samoluk, Sergio Sebastián; Neffa, Viviana Solís; Seijo, Guillermo

doi:10.1007/s10265-015-0756-1

Cited by 7 publications

(4 citation statements)

References 59 publications

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“…The establishment of species relationships through chromosomal analysis is based on the premise that closely related species may have more similar karyotypes than distantly related ones (Acosta et al 2016;Chalup et al 2015;Robledo et al 2009;Seijo et al 2004;Silvestri et al 2015;Weiss-Schneeweiss et al 2008). Here, we combined DAPI/CMA banding and FISH techniques to generate useful chromosomal markers for the identification of chromosome homeologies among the three rhizomatous tetraploid species and between them and the diploid species from different sections of the genus Arachis.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic evidences on the evolutionary relationships between the tetraploids of the section Rhizomatosae and related diploid species (Arachis, Leguminosae)

et al. 2017

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Rhizomatosae is a taxonomic section of the South American genus Arachis, whose diagnostic character is the presence of rhizomes in all its species. This section is of particular evolutionary interest because it has three polyploid (A. pseudovillosa, A. nitida and A. glabrata, 2n = 4x = 40) and only one diploid (A. burkartii, 2n = 2x = 20) species. The phylogenetic relationships of these species as well as the polyploidy nature and the origin of the tetraploids are still controversial. The present study provides an exhaustive analysis of the karyotypes of all rhizomatous species and six closely related diploid species of the sections Erectoides and Procumbentes by cytogenetic mapping of DAPI/CMA heterochromatin bands and 5S and 18-26S rDNA loci. Chromosome banding showed variation in the DAPI heterochromatin distribution pattern, which, together with the number and distribution of rDNA loci, allowed the characterization of all species studied here. The bulk of chromosomal markers suggest that the three rhizomatous tetraploid species constitute a natural group and may have at least one common diploid ancestor. The cytogenetic data of the diploid species analyzed evidenced that the only rhizomatous diploid species-A. burkartii-has a karyotype pattern different from those of the rhizomatous tetraploids, showing that it is not likely the genome donor of the tetraploids and the non-monophyletic nature of the section Rhizomatosae. Thus, the tetraploid species should be excluded from the R genome, which should remain exclusively for A. burkartii. Instead, the karyotype features of these tetraploids are compatible with those of different species of the sections Erectoides and Procumbentes (E genome species), suggesting the hypothesis of multiple origins of these tetraploids. In addition, the polyploid nature and the group of diploid species closer to the tetraploids are discussed.

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Section: Discussionmentioning

confidence: 99%

Cytogenetic evidences on the evolutionary relationships between the tetraploids of the section Rhizomatosae and related diploid species (Arachis, Leguminosae)

et al. 2017

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“…However, L. odoratus L. bears microsatellites in two pairs of chromosomes (nos. 3 and 5) via the karyotype analysis by [ 11 , 57 ] studied 14 species of Lathyrus (included L. odoratus L. and L. paranensis Burkart) by double FISH to determine the 45S and the 5S rDNA loci in addition to the CMA and DAPI banding patterns, they analyzed too the karyotypes in relation to geographic and climatic changes. In their investigation, they detected two loci of 45S rDNA on the short arm of chromosomes no.…”

Section: Discussionmentioning

confidence: 99%

Ribosomal DNA localization on Lathyrus species chromosomes by FISH

Ali

Osman

2020

Journal of Genetic Engineering and Biotechnology

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Background Fluorescence In Situ Hybridization (FISH) played an essential role to locate the ribosomal RNA genes on the chromosomes that offered a new tool to study the chromosome structure and evolution in plant. The 45S and 5S rRNA genes are independent and localized at one or more loci per the chromosome complement, their positions along chromosomes offer useful markers for chromosome discriminations. In the current study FISH has been performed to locate 45S and 5S rRNA genes on the chromosomes of nine Lathyrus species belong to five different sections, all have chromosome number 2n=14, Lathyrus gorgoni Parl, Lathyrus hirsutus L., Lathyrus amphicarpos L., Lathyrus odoratus L., Lathyrus sphaericus Retz, Lathyrus incospicuus L, Lathyrus paranensis Burkart, Lathyrus nissolia L., and Lathyrus articulates L. Results The revealed loci of 45S and 5S rDNA by FISH on metaphase chromosomes of the examined species were as follow: all of the studied species have one 45S rDNA locus and one 5S rDNA locus except L. odoratus L., L. amphicarpos L. and L. sphaericus Retz L. have two loci of 5S rDNA. Three out of the nine examined species have the loci of 45S and 5S rRNA genes on the opposite arms of the same chromosome (L. nissolia L., L. amphicarpos L., and L. incospicuus L.), while L. hirsutus L. has both loci on the same chromosome arm. The other five species showed the loci of the two types of rDNA on different chromosomes. Conclusion The detected 5S and 45S rDNA loci in Lathyrus could be used as chromosomal markers to discriminate the chromosome pairs of the examined species. FISH could discriminate only one chromosome pair out of the seven pairs in three species, in L. hirsutus L., L. nissolia L. and L. incospicuus L., and two chromosome pairs in five species, in L. paranensis Burkart, L. odoratus L., L. amphicarpos L., L. gorgoni Parl. and L. articulatus L., while it could discriminate three chromosome pairs in L. sphaericus Retz. these results could contribute into the physical genome mapping of Lathyrus species and the evolution of rDNA patterns by FISH in the coming studies in future.

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“…Though the polymorphism can be observed in the amount, type and distribution of heterochromatin but the general patterns were relatively unique for each species studied as reported in Vigna [39,40], Citrus [41], Cestrum [42] and Abelmoschus [16]. taxonomic value at the species level [43], leading to greater genome size [1]. The polymorphism of the heterochromatic-rich repetitive fractions advocates their important role in genome structure and function of the genus Curcuma.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Heterochromatin Distribution Pattern in Wild and Cultivated Species of Curcuma L.

JM¹,

DR²

2016

Med Aromat Plants

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Fluorochrome banding pattern with CMA and DAPI showed variation in the type, amount and distribution of heterochromatin in all the Curcuma species studied. The study provided clear insight on the numbers and genomic components such as the repetitive (GC, AT and GC/AT-rich) and non-repetitive DNA fraction(s). The polymorphism in heterochromatic-rich repetitive fraction(s) reflects higher molecular organization of the genome and evolutionary status of the genus Curcuma. The present works entails the importance of heterochromatin rich-repetitive DNA fraction(s) which play significant role in genome organization and evolutionary pattern in the genus Curcuma.

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Karyotype characterization and evolution in South American species of Lathyrus (Notolathyrus, Leguminosae) evidenced by heterochromatin and rDNA mapping

Cited by 7 publications

References 59 publications

Cytogenetic evidences on the evolutionary relationships between the tetraploids of the section Rhizomatosae and related diploid species (Arachis, Leguminosae)

Cytogenetic evidences on the evolutionary relationships between the tetraploids of the section Rhizomatosae and related diploid species (Arachis, Leguminosae)

Ribosomal DNA localization on Lathyrus species chromosomes by FISH

Comparative Analysis of Heterochromatin Distribution Pattern in Wild and Cultivated Species of Curcuma L.

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