Karyotype and nuclear DNA content variation in Passiflora L

Ferreira, Darley Aparecido Tavares; Fontes, Milene Miranda Praça; Vieira, Ariane Tonetto; Nunes, Ana Paula Ferreira; Clarindo, Wellington Ronildo

doi:10.1016/j.scienta.2020.109532

Cited by 7 publications

(5 citation statements)

References 39 publications

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“…Very accurate long-read data can also be used for this estimation ( 86 ). When polyploid signals remain challenging to resolve from short reads alone, additional assessments with flow cytometry and/or karyotyping methods have tremendous utility ( 16 , 87 ). The quantity and composition of the repetitive content can also inform the optimal long-read versus short-read strategy.…”

Section: A Roadmap For Moving Forward With Green Plantsmentioning

confidence: 99%

Green plant genomes: What we know in an era of rapidly expanding opportunities

Kress

Soltis

Kersey

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Green plants play a fundamental role in ecosystems, human health, and agriculture. As de novo genomes are being generated for all known eukaryotic species as advocated by the Earth BioGenome Project, increasing genomic information on green land plants is essential. However, setting standards for the generation and storage of the complex set of genomes that characterize the green lineage of life is a major challenge for plant scientists. Such standards will need to accommodate the immense variation in green plant genome size, transposable element content, and structural complexity while enabling research into the molecular and evolutionary processes that have resulted in this enormous genomic variation. Here we provide an overview and assessment of the current state of knowledge of green plant genomes. To date fewer than 300 complete chromosome-scale genome assemblies representing fewer than 900 species have been generated across the estimated 450,000 to 500,000 species in the green plant clade. These genomes range in size from 12 Mb to 27.6 Gb and are biased toward agricultural crops with large branches of the green tree of life untouched by genomic-scale sequencing. Locating suitable tissue samples of most species of plants, especially those taxa from extreme environments, remains one of the biggest hurdles to increasing our genomic inventory. Furthermore, the annotation of plant genomes is at present undergoing intensive improvement. It is our hope that this fresh overview will help in the development of genomic quality standards for a cohesive and meaningful synthesis of green plant genomes as we scale up for the future.

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Section: A Roadmap For Moving Forward With Green Plantsmentioning

confidence: 99%

Green plant genomes: What we know in an era of rapidly expanding opportunities

Kress

Soltis

Kersey

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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“…Carbol-fuchsin was used for staining. Photos were taken under a 100Â oil lens (Chen 2003;Ferreira et al 2020;Qiao et al 2020). The chromosome length and arm ratio were calculated, and chromosome type and karyotype classification were judged according to the average chromosome of 10-20 cells selected.…”

Section: Experimental Methodsmentioning

confidence: 99%

A taxonomic study on pollen morphology, karyotype features, floral and fruit pigment components of 13 taxa of Lonicera L.

Zhai²,

Sun

et al. 2023

Palynology

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Due to extensive introduction and breeding, the taxonomic relationships of Lonicera L. are unclear. Alternative scientific methods are needed to explore the classification of these new Lonicera. In this paper, the pollen morphology characteristics, karyotype features, and floral and fruit pigment components of 13 taxa of Lonicera were analyzed by means of scanning electron microscopy, karyotype analysis, High Performance Liquid Chromatography Mass Spectrometry (HPLC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS). The 13 representatives of Lonicera were divided into groups of four, three, three and three taxa through the analysis of pollen morphology characteristics, karyotype features, floral composition and fruit pigment composition, respectively. Lonicera ruprechtiana and Lonicera tatarica always clustered together. Five hybrids, including Lonicera korolkow × Lonicera maackii and Lonicera korolkow × Lonicera tatarica No. 8 can generally be clustered with their parents. These results indicate that the analysis of pollen morphology characteristics, karyotype features, and floral and fruit pigment composition has value and significance for the study of systematic classification and relationships, and the conclusions of this study partially support morphological taxonomy.

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“…Chromosome karyotype characteristics of Lonicera L.: Refer to the root tip squashing method for sampling, fixation, dissociation, staining, measuring and photographing (Parrish et al 2021;Wu et al 2021). When the root length of Lonicera L. seeds was up to 0.5-1.0 cm, 20 root tips were taken and pressed into slices; the numbers of clear and scattered chromosomes in the cells was counted when more than 85% of the cells have a constant and consistent chromosome numbers in 20 cells in each species Lonicera L., then the photos were taken under the 100 times oil lens (Ferreira et al 2020;Ruiyang Chen 2003;Yonggang Qiao et al 2020). The chromosome length and arm ratio were calculated and chromosome type and karyotype classification were judged according to the average chromosome of 10-20 cells selected; when the indexes are quantified, the quantitative indexes take their values, and the qualitative indexes are assigned value to 1, 2 ,...n, according to their different forms.…”

Section: Experimental Methodsmentioning

confidence: 99%

A taxonomic study on pollen morphology characteristics, chromosome karyotype characteristics, floral and fruit pigment compositions of 13 species and hybrids of Lonicera L

Li¹,

Zhai²,

Li³

et al. 2022

Preprint

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In this paper, the pollen morphology characteristics, chromosome karyotype characteristics, floral and fruity pigment compositions of 13 species and hybrids of Lonicera L. were analyzed by means of scanning electron microscopy, root tip squash method, HPLC-MS and GC-MS, and then they were classified separately to discuss their genetic relationship and taxonomic relationship. According to the morphological taxonomy, Lonicera L. distributed in northern are divided into two categories by whether the branchlets are hollow or not. In this experiment, Lonicera chrysanthaTurcz. ex Ledeb., Lonicera maackii (Rupr.) Maxim., Lonicera ruprechtiana Regel., Lonicera tatarica Linn. and Lonicera korolkowi ‘Zabelii’. belongsto the category of hollow branchlets; and Lonicera edulisTurcz. and Lonicera tatarinowiiMaxim. belongs to the category of branchlets with solid white pith. Through the analysis of pollen morphology characteristics, chromosome karyotype characteristics, floral and fruit pigment compositions, and using the above four indexes for clustering, Lonicera ruprechtiana Regel. and Lonicera tatarica Linn. can always be clustered together. Five hybrids such as Lonicera korolkow‘Zabelii’.×Lonicera maackii (Rupr.) Maxim., Lonicera korolkow‘Zabelii’.×Lonicera tatarica Linn.No.8 etal. can basically clustered with their parents, which is conform to the morphological taxonomy. These results indicate that the analysis of pollen morphology characteristics, chromosome karyotype characteristics, floral and fruit pigment compositions, has certain value and significance for the study of system classification and genetic relationship of Lonicera L., and the conclusions of this study partially support morphological taxonomy. Among the four clusters, the clustering result of pollen morphology characteristics was in the highest agreement with morphological taxonomy.

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Karyotype and nuclear DNA content variation in Passiflora L

Cited by 7 publications

References 39 publications

Green plant genomes: What we know in an era of rapidly expanding opportunities

Green plant genomes: What we know in an era of rapidly expanding opportunities

A taxonomic study on pollen morphology, karyotype features, floral and fruit pigment components of 13 taxa of Lonicera L.

A taxonomic study on pollen morphology characteristics, chromosome karyotype characteristics, floral and fruit pigment compositions of 13 species and hybrids of Lonicera L

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