Karyotype analysis of Egyptian henbane, Hyoscyamus muticus L.

Tyagi, B. R.; Dubey, Ratna

doi:10.1508/cytologia.54.313

Cited by 7 publications

(9 citation statements)

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“…Hyoscyamus muticus of 2n=28 (Srivastava and Lavania 1987;Tyagi and Dubey 1989;Misra et al 1991;Badr et al 1997 andMohamed 1997). Only Bahl and Tyagi (1992) reported that H. muticus had 2n=30.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetical and ecological studies of some wild congeneric species in the Solanaceae distributed in upper Egypt

2010

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ABSTRACT.Cytogenetical and ecological studies in some species of the Solanaceae were made in the Mediterranean-type ecosystem in Upper Egypt. The chromosome complement of Datura innoxia (2n=24) consisted of 24 median-centromeric chromosomes; that of D. stramonium (2n=24) consisted of 22 median-and two submedian-centromeric chromosomes; that of Hyoscyamus albus (2n=68) consisted of 62 median-and six submedian-centromeric chromosomes, that of H. muticus (2n=28) consisted of 21 median-and seven submedian-centromeric chromosomes; that of Withania somnifera (2n=36; new count) consisted of 27 median-and nine submedian-centromeric chromosomes; and that of Solanum nigrum (2n=72) consists of 68 median and four submedian-centromeric chromosomes. Thirty-three reproducible polymorphic bands were resulted after four RAPD-PCR primers; those bands were used for studying the genetics similarity among the species. Average similarity coeffi cient was ranged from 0.05 to 0.47. RAPD bands resulted after PCR: D. innoxia and D. stramonium showed fi ve bands each; H. albus and H. muticus showed two and one bands respectively; W. somnifera seven bands and S. nigrum showed 13 bands, different patterns were produced among congeneric species. Ionic status of hot water extract of the species revealed that Hyoscyamus species was more accumulated Na + and Ca ++ , while Datura species accumulated more K + and Mg ++ and W. somnifera and S nigrum were intermediate. These strategies of ionic accumulation were in relation to the different chromosome number of species, which might help for surviving the species in desert ecosystem.

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Section: Discussionmentioning

confidence: 99%

Cytogenetical and ecological studies of some wild congeneric species in the Solanaceae distributed in upper Egypt

2010

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“…Its somatic metaphase chromosomes are small (2.14-3.88 ,um) and of relatively uniform size (Tyagi and Dubey 1989). A conventional karyotype of somatic metaphase chromosomes of H. muticus after acetocarmine staining has been presented by Tyagi and Dubey (1989), but only a few of the chromosomes of the haploid complement could be reliably identified.…”

Section: Discussionmentioning

confidence: 99%

“…However, when C-bands coupled with other characteristics such as chromosome length and arm ratio, they proved valuable in positive identification of all the 14 chromosomes of H. muticus. Although, a number of primary trisomics and translocation stocks have been established in H. muticus (Tyagi 1990, Tyagi andBahl 1992), the lack of definitive somatic karyotype has precluded their use in placing gene(s)/marker(s) and linkage groups onto known chromosomes and in testing their independence. In spite of certain limitations, pachytene chromosome analysis remains a very powerful cytogenetic tool.…”

Section: Discussionmentioning

confidence: 99%

“…In spite of certain limitations, pachytene chromosome analysis remains a very powerful cytogenetic tool. It has been widely applied in various plant species (McClintock 1929, Barton 1950, Jacob 1956, Gillies 1968, Ramanna and Wagenvoort 1976, Nakamura and Tsuchiya 1982, Dundas et al 1983, Khush et al 1984, Singh and Hymowitz 1988, Tyagi and Dubey 1990, Wu 1993, PintoMaglio and Cruz 1998. Pachytene chromosomes are more extended, paired (haploid number) and highly differentiated, and such criteria as relative length, position of the centromere and distribution pattern of heterochromatin may be more readily applied to the identification of the chromosomes at this stage than is possible in the somatic complement.…”

Section: Discussionmentioning

confidence: 99%

“…An elite inbred-CIMAP/NP-41 of H. muticus developed by Tyagi (1986) was chosen for study. Flower buds, at the appropriate stage of development, were collected from field grown healthy plants and fixed in a freshly prepared mixture of 95% ethanol : chloroform : glacial acetic acid (6 : 3 : 1 v/v).…”

Section: Methodsmentioning

confidence: 99%

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