Karyotype Analysis and Chromosomal Distribution of Repetitive DNA Sequences of &lt;b&gt;&lt;i&gt;Cucumis metuliferus&lt;/i&gt;&lt;/b&gt; Using Fluorescence in situ Hybridization

Yagi, Kouhei; Siedlecka, Ewa; Pawełkowicz, Magdalena; Wojcieszek, Michał; Przybecki, Zbigniew; Tagashira, Norikazu; Hoshi, Yoshikazu; Malepszy, S.; Pląder, Wojciech

doi:10.1159/000369183

Cited by 8 publications

(7 citation statements)

References 21 publications

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“…In another phylogenetic study, it was suggested that C. metuliferus may be derived from the African Cucumis species, and thus may be a putative diploid ancestral species of the C. heptadactylus group [Sebastian et al, 2010]. The 45S rDNA and 5S rDNA of C. metuliferus was mapped previously on chromosomes 11 and 12 [Yagi et al, 2014]. In our study, chromosomes 11 and 12 of C. metuliferus were found to be rch type I and II, respectively.…”

Section: Discussionsupporting

confidence: 61%

“…The probes were directly labeled with tetramethyl-rhodamine-5-dUTP (red) (Roche Diagnostics, Mannheim, Germany) using a nick translation kit (Roche Diagnostics). The chromosomal preparation and FISH methods were performed following Yagi et al [2014]. After checking the locations of the signals, blended probes were used for signal detection.…”

Section: Chromosomal Preparation and Fishmentioning

confidence: 99%

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Molecular Cytogenetic Analysis of Cucumis Wild Species Distributed in Southern Africa: Physical Mapping of 5S and 45S rDNA with DAPI

Yagi

Pawełkowicz²,

Osipowski³

et al. 2015

Cytogenet Genome Res

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Wild Cucumis species have been divided into Australian/Asian and African groups using morphological and phylogenetic characteristics, and new species have been described recently. No molecular cytogenetic information is available for most of these species. The crossability between 5 southern African Cucumis species (C. africanus, C. anguria, C. myriocarpus, C. zeyheri, and C. heptadactylus) has been reported; however, the evolutionary relationship among them is still unclear. Here, a molecular cytogenetic analysis using FISH with 5S and 45S ribosomal DNA (rDNA) was used to investigate these Cucumis species based on sets of rDNA-bearing chromosomes (rch) types I, II and III. The molecular cytogenetic and phylogenetic results suggested that at least 2 steps of chromosomal rearrangements may have occurred during the evolution of tetraploid C. heptadactylus. In step 1, an additional 45S rDNA site was observed in the chromosome (type III). In particular, C. myriocarpus had a variety of rch sets. Our results suggest that chromosomal rearrangements may have occurred in the 45S rDNA sites. We propose that polyploid evolution occurred in step 2. This study provides insights into the chromosomal characteristics of African Cucumis species and contributes to the understanding of chromosomal evolution in this genus.

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Section: Discussionsupporting

confidence: 61%

Section: Chromosomal Preparation and Fishmentioning

confidence: 99%

Molecular Cytogenetic Analysis of Cucumis Wild Species Distributed in Southern Africa: Physical Mapping of 5S and 45S rDNA with DAPI

Yagi

Pawełkowicz²,

Osipowski³

et al. 2015

Cytogenet Genome Res

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“…In Cucumis, studies involving molecular cytogenetics were mainly focused on important cultivated crops (e.g., cucumbers, melons) (Chen et al 1998;Han et al 2008;Liu et al 2010). Few relevant studies have been reported concerning the genome organization and chromosomal structure of wild species, except for some concerning chromosome morphology and karyotype (Chen et al 1999;Zhao et al 2011;Yagi et al 2014Yagi et al , 2015. Most wild species of Cucumis have small chromosomes, which makes traditional cytogenetics methods, like banding, not suitable for Cucumis chromosome analysis (difficult to stain).…”

Section: Introductionmentioning

confidence: 99%

Comparative chromosomal localization of 45S and 5S rDNAs and implications for genome evolution inCucumis

Zhang

Yang

et al. 2016

Genome

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Ribosomal DNAs are useful cytogenetic markers for chromosome analysis. Studies investigating site numbers and distributions of rDNAs have provided important information for elucidating genome organization and chromosomal relationships of many species by fluorescence in situ hybridization. But relevant studies are scarce for species of the genus Cucumis, especially in wild species. In the present study, FISH was conducted to investigate the organization of 45S and 5S rDNA among 20 Cucumis accessions, including cultivars and wild accessions. Our results showed that the number of 45S rDNA sites varied from one to five pairs in different accessions, and most of these sites are located at the terminal regions of chromosomes. Interestingly, up to five pairs of 45S rDNA sites were observed in C. sativus var. sativus, the species which has the lowest chromosome number, i.e., 2n = 14. Only one pair of 5S rDNA sites was detected in all accessions, except for C. heptadactylus, C. sp, and C. spp that had two pairs of 5S rDNA sites. The distributions of 5S rDNA sites showed more variation than 45S rDNA sites. The phylogenetic analysis in this study showed that 45S and 5S rDNA have contrasting evolutionary patterns. We find that 5S rDNA has a polyploidizationrelated tendency towards the terminal location from an interstitial location but maintains a conserved site number, whereas the 45S rDNA showed a trend of increasing site number but a relatively conserved location.

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“…Chromosome painting clones enable ancestral genome recognition in allopolyploid species. Zhang et al [ 45 ] identified sequences that distinguish the three genomes of Triticum aestivum , and in Cucumis metuliferus a species-specific repetitive sequence (pMetSat) has been identified by Yagi et al [ 46 ]. In our study, we observed a hybridization signal of the chromosome-like painting clone L16 Fp04 only in F .…”

Section: Discussionmentioning

confidence: 99%

Exploiting repetitive sequences and BAC clones in Festuca pratensis karyotyping

et al. 2017

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The Festuca genus is thought to be the most numerous genus of the Poaceae family. One of the most agronomically important forage grasses, Festuca pratensis Huds. is treated as a model plant to study the molecular mechanisms associated with tolerance to winter stresses, including frost. However, the precise mapping of the genes governing stress tolerance in this species is difficult as its karyotype remains unrecognized. Only two F. pratensis chromosomes with 35S and 5S rDNA sequences can be easily identified, but its remaining chromosomes have not been distinguished to date. Here, two libraries derived from F. pratensis nuclear DNA with various contents of repetitive DNA sequences were used as sources of molecular probes for fluorescent in situ hybridisation (FISH), a BAC library and a library representing sequences most frequently present in the F. pratensis genome. Using FISH, six groups of DNA sequences were revealed in chromosomes on the basis of their signal position, including dispersed-like sequences, chromosome painting-like sequences, centromeric-like sequences, knob-like sequences, a group without hybridization signals, and single locus-like sequences. The last group was exploited to develop cytogenetic maps of diploid and tetraploid F. pratensis, which are presented here for the first time and provide a remarkable progress in karyotype characterization.

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Karyotype Analysis and Chromosomal Distribution of Repetitive DNA Sequences of <b><i>Cucumis metuliferus</i></b> Using Fluorescence in situ Hybridization

Cited by 8 publications

References 21 publications

Molecular Cytogenetic Analysis of <b><i>Cucumis</i></b> Wild Species Distributed in Southern Africa: Physical Mapping of 5S and 45S rDNA with DAPI

Molecular Cytogenetic Analysis of <b><i>Cucumis</i></b> Wild Species Distributed in Southern Africa: Physical Mapping of 5S and 45S rDNA with DAPI

Comparative chromosomal localization of 45S and 5S rDNAs and implications for genome evolution inCucumis

Exploiting repetitive sequences and BAC clones in Festuca pratensis karyotyping

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