Summary Karyotype analysis in 9 species of Jute (Corchorus, Family. Tiliaceae) is performed through Image Analyzing System with the objective to get accurate numerical data from metaphase plate microphotographs for ascertaining interrelationship among/between the species following clustering analysis. Karyotypes revealed (2nϭ14) 3 (C. fascicularis), 2 (C. capsularis, C. olitorius, C. aestuans, C. pseudocapsularis, C. pseudoolitorius) and 1 (C. tridens, C. trilocularis and C. urticaefolius) morphologically distinct chromosome types in the genus with prevalence of median chromosomes, mostly graded (evidenced from relative chromosome length) and symmetrics. C. fascicularis also showed 2nϭ28 (11.54%) chromosomes. Absolute chromosome length among the species varied from 1.37 mm to 3.50 mm. Haploid chromatin length (11.97 mm-17.72 mm) ranged significantly (pϽ0.001) among the species. An identification key of the species has been formulated on the basis of karyomorphological data. A dendrogram was constructed and it showed close relationship between C. olitorius and C. aestuans and among C. pseudoolitorius, C. trilocularis and C. urticaefolius. A complex evolution in the genus was suggested.