Karyology of Mugil Liza and M. Curema From Venezuela

Abstract

Karyotypes of Mugil liza and M. curema from Venezuela were studied. M. liza karyotype is 2n=48 acrocentric chromosomes, but M. curema is 2n=24, containing one submetacentric and 11 metacentric pairs. The chromosomic complement described for M. liza constitutes the first report for the speciesand coincides with the modal karyotype (2n=48) in the Mugilidae family. Venezuelan M. curema karyotype differs in number and shape from M. curema from Louisiana (USA), which has been reported to have a complement 2n=28 (10 metacentric pairs, two subtelocentric pairs and two acrocentric pairs).

The Mugil curema species complex (Pisces, Mugilidae): a new karyotype for the Pacific white mullet mitochondrial lineage

“…Even among the 15 cryptic species identified within the Mugil cephalus species complex (Durand and Borsa 2015), the six cytogenetically investigated lineages share not only the chromosome formula but also the major cytogenetic features (see Rossi et al 2016 for references). The only exceptions to this picture of 2n = 48 are represented by Liza (currently Planiliza) abu (Heckel, 1843) (Değer et al 2013) and the two “ M. curema ” lineages so far investigated (LeGrande and Fitzsimons 1976, Nirchio and Cequea 1998, Nirchio et al 2005). Planiliza abu (Değer et al 2013), which has a limited Asian distribution, shows a karyotype characterized by 2 large metacentric and 46 subtelo-, acrocentric chromosomes, a diploid number of 48 and FN = 50; a pericentric inversion is invoked to interpret the origin of the metacentric chromosome pair.…”

“…Cell suspensions were obtained from the cephalic kidney, following the procedure reported by Nirchio and Oliveira (2006). Nucleolus organizer regions (NORs) were identified by silver (Ag) nitrate staining (Howell and Black 1980), and C-banding patterns were obtained following the protocol described by Sumner (1972).…”

“…Although only two of the four mitochondrial lineages of “ M. curema ” have been cytogenetically investigated to date, they have been found to differ from each other in diploid number and chromosome formula, as well as differing from all the other mugilids investigated to date (see Rossi et al 2016 for a review). M. curema sensu strictu shows a karyotype composed of 2n = 28 chromosomes (LeGrande and Fitzsimons 1976, Nirchio et al 2005) and M. margaritae shows a karyotype composed of 2n = 24 chromosomes (Nirchio and Chequea 1998, Nirchio et al 2005). …”

Karyological Characterization of Three Fish Species (Family: Characidae)

“…They were caught and transported to the lab and kept alive until processed. Mitotic chromosomes were prepared from head kidney, liver and gills as described by Nirchio & Cequea [1998]. Each specimen was injected with 0.05% Colchicines (1ml / 100g fish weight) the fish were maintained in a well aerated aquarium and after 2hr they were sacrificed.…”

Identificación molecular de especies crípticas de Mugil curema (Pisces: Mugilidae) en las costas de México

“…El primer linaje fue hallado en Brasil el cual tiene un número cromosómico 2n=28, citotipo I (Nirchio et al, 2005) considerado como la verdadera M. curema reportada por LeGrande & Fitzmons (1976) nombrado provisionalmente "T2" por Durand & Borsa (2015), y que coincide con el tipo II de Fraga et al (2007); todo el Golfo de México pertenece a este linaje. El segundo cariotipo (2n=24, citotipo II), fue encontrado en Venezuela (Nirchio & Cequea, 1998;Nirchio et al, 2003) el cual corresponde con el segundo linaje nombrado inicialmente como "Mugil spp. N" por Durand & Borsa (2015) y actualmente como Mugil margaritae (Menezes et al, 2015).…”