With 3 plates and 2 figures in the text)We define the species boundaries of white-toothed shrews (genus Crocidura) in Taiwan using karyological and morphological characteristics. Ninety-nine animals were obtained from all over Taiwan at capture rates usually less than 10%. Three species are recognized by distinct cytotypes:Crociduru uttenuata tanakae 2n = 40, FN = 56; Crocidura suaveolens hoslefti 2n = 40, FN = 50; Crocidura kurodai 2n = 40, FN = 54. A suite of six morphological characters diagnose the three species: shape of skull, position of incisive foramina, shape of fourth upper premolar, shape of pinna, tail vibrissae, and foot pads. A species key and notes on the life history of each species are provided. Finally, we discuss chromosomal evolution and biogeography of Crociduru in East and South East Asia.