Karyological Analysis and Morphometrics of the Lesser Asiatic House Bat, <i>Scotophilus kuhlii</i> (Chiroptera, Vespertilionidae)

Abstract

Summary Karyological analysis and morphometrics of the lesser Asiatic house bat (Scotophilus kuhlii) from Thailand were studied. Blood samples were taken from 5 male and 5 female bats, lymphocytes were cultured at 37 C for 72 h in presence of colchicine, and metaphase spreads were performed on microscopic slides and air-dried. Conventional staining and GTG-banding techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of S. kuhlii was 2n=36, and the fundamental number (NF) was 52 in both male and female. The types of autosomes were 6 large metacentric, 4 large submetacentric, 6 large telocentric, 2 medium submetacentric, 10 medium telocentric, 2 small metacentric, and 4 small telocentric chromosomes. The X chromosome was a large submetacentric chromosome and the Y chromosome was the smallest acrocentric chromosome. The GTG-banding technique, indicated that the number of bands and locations in S. kuhlii was 167, and that each chromosome pair could be clearly differentiated. In addition, a pair of long arms near the centromere of chromosome pair 17 showed clearly observable nucleolar organizer regions (NORs). In terms of morphological measurement, the following measurements were taken 6 external morphological characters as well as 13 cranial and dental characters. The karyotype formula of S. kuhlii was as follows:

Chromosome Analysis and Morphometric of Croslet Horseshoe Bat, <i>Rhinolophus coelophyllus</i> and Least Horseshoe Bat, <i>Rhinolophus pusillus</i> (Chiroptera, Rhinolophidae)

“…NORs produce numerous gene expressions, and they are composed of more non-histone protein than other chromosome regions. These proteins induce specific dark bands (NOR positive) by the reduction of organic silver that changes silver to be dark (Gold (Supanuam et al 2012), and H. larvatus (2n=32) had two NORs positions on the long arm near the centromere of large metacentric chromosome pair 8 (Jantarat et al 2014). Furthermore, NORs are usually located close to a subcentromeric region of a chromosome arm.…”

Chromosome Analysis and Morphometric of Intermediate Roundleaf Bat, Hipposideros larvatus (Chiroptera, Hipposideridae) by Conventional, GTG-banding and Ag-NOR Banding Techniques

“…The order Chiroptera is an interesting group with chromosome evolution ranging from considerably conservative to quite divergent (Varella-Garcia et al 1989). Chromosomal studies of bats from Thailand have reported only 36 species representing seven families (Harada et al 1982, Hood and Baker 1986, Harada et al 1985a, 1985b, Bickham et al 1986, Hood et al 1988, Wu et al 2009, Supanuam et al 2012.…”