KARYOKINESIS OF THE SOMATIC NUCLEUS OF <i>ASPERGILLUS NIDULANS</i>. I. THE JUVENILE CHROMOSOME CYCLE (FEULGEN STAINING)

Weijer, J.; Weisberg, S. H.

doi:10.1139/g66-046

Cited by 19 publications

(6 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The centriolar bodies of N. coryli are Feulgen positive. That centrioles may contain DNA has also been shown in Feulgen stained slides of Neurospora crassa (Weijer, Koopmans & Weijer, 1963McDonald & Weijer, 1966) and of Aspergillus nidulans (Weijer & Weisberg, 1966). By applying acridine-orange fluorescence to isolated basal bodies of the Ciliate Paramaecium, Smith-Sonneborn & Plaut (1967) confirmed the results found in Tetrahymena by Seaman (1960) and Randall & Disbrey (1965) that basal bodies (kinetosomes) contain DNA.…”

Section: Centriolesmentioning

confidence: 61%

“…For fixing and staining several methods were tested. The following method gave the most satisfying results: a sample of N. coryli was diluted with distilled water (1:3) and dried on slides for 30-45 minutes at 60°C (Weijer & Weisberg, 1966). The slides were transferred to Carnoy's (6:3:1) mixture for 45 minutes, hydrolyzed during 20 minutes in 1 N HC1 at 60°C, stained overnight with leuco-basic-fuchsin (pH 3.5) and embedded in Euparal.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A cytological study of Nematospora coryli pegl

Koopmans

1977

Genetica

View full text Add to dashboard Cite

Section: Centriolesmentioning

confidence: 61%

Section: Methodsmentioning

confidence: 99%

A cytological study of Nematospora coryli pegl

Koopmans

1977

Genetica

View full text Add to dashboard Cite

“…The linear arrangement of the chromosomes observed during division phases in Penicillium (LAANE, 1967) have previously been described in Neurospora and Aspergillus (see WEIJER and WEISBERG, 1966). Similar arrangements have been found in very specialized cells (spermatid cells of the Chinese hamster Cricetulus barabensis) by DOUGLAS (1965), and in so different organisms as the cave cricket (INOE and SATO ,1962), in the grasshopper Romneln microptern (TAY-LOR, 1964), in Hepaticue (RIETBERGER, 1964), and in icerynie coccids ( HUGHES-SCHRADER, 1946).…”

Section: Discussionmentioning

confidence: 74%

Further Cytological Studies in the Genus Penicillium Link

Laane

2009

Hereditas

View full text Add to dashboard Cite

“…In the uninucleate cells of eukaryote organisms, mitosis and cell division are coordinated both in time and position. In Aspergillus nidulans, a fungus genetically similar (apart from its perfect stage) to Verticillium, nuclear division (Robinow & Caten, 1969;Weijer & Weisberg, 1966;Rosenberger & Kessel, 1967) and DNA synthesis (Kessel & Rosenberger, 1968 ;Bainbridge, 1971) are normally synchronized, or at least partially synchronized, when the fungus is growing on a rich medium; nuclear divisions are followed by the formation of a series of septae (Clutterbuck, 1970;Bainbridge, 1976). The timing of nuclear division during germination has been studied in A. nidulans and Fusarium oxysporum (Bainbridge, 1971 ;Kumari et al, 1975).…”

Section: Introductionmentioning

confidence: 99%

DNA Content of Germinating Spores, Individual Hyphal Cells and Resting Structure Cells of Verticillium spp. Measured by Microdensitometry

Typas

Heale

1980

Microbiology

View full text Add to dashboard Cite

Using microdensitometry, the cell cycle was investigated in large populations (500) of Feulgen-stained nuclei in conidia, hyphae and resting structures of various strains (all haploid) of Verticillium albo-atrum and V. dahliae as well as a stable diploid strain, V. dahliae var. longisporum. When uninucleate conidia, harvested from 3 d Roux bottle agar cultures, were incubated in stationary liquid complete medium at 24 "C in the dark, they exhibited partial synchrony for germ-tube emergence (50 % emergent at about 7 h, reaching 95 % at 10 to 12 h), DNA replication (50 % increase at about 7.5 h) and mitosis (50 % at about 10 h, reaching 95 % at 12 h); the second nuclear division occurred at 21 to 24 h.Significant amounts of DNA replication were not detected until 6 h ; maximum levels, i.e. a doubling of DNA content (in nuclei not yet divided), occurred at 10 h. There was a 3 h G1 phase between the first and second nuclear divisions ; the S and G2 phases were estimated at about 30 and 90 min duration, respectively. The absolute DNA content per nucleus for ungerminated conidia of one strain of V. dahliae was found to be 0.025 to 0.030pg. In growing hyphae, only the apical cell(s) contained two or more nuclei; DNA levels approximated to those for ungerminated conidia (Gl) but a small number of nuclei had begun the S phase. In hyphal tip regions comprising up to 10 cells, a synchronous nuclear division was observed, and nuclei in the apical cells often had higher DNA values than other nuclei in the same hypha. Cells of young microsclerotia (6 to 8 d-old) of V. dahliae and resting mycelium (9 to 12d-old) of V. albo-atrum were haploid and uninucleate with a small minority of the nuclei in the S phase; no evidence for diploidy or endopolyploidy was found at these sampling levels. g i l h nidulans and Alternaria solani hyphae from radial growth rates and ranges in apical cell length. Journal of General Microbiology 97, CLUTTERBUCK, A. J. (1969). Cell volume per nucleus in haploid and diploid strains of Aspergillus nidulans. Journal of General Microbiology 55, CLUTTERBUCK, A. J. (1970). Synchronous nuclear division and septation in Aspergillus nidulans. Journal of General Microbiology 60, 133-135.

show abstract

KARYOKINESIS OF THE SOMATIC NUCLEUS OF ASPERGILLUS NIDULANS. I. THE JUVENILE CHROMOSOME CYCLE (FEULGEN STAINING)

Cited by 19 publications

References 12 publications

A cytological study of Nematospora coryli pegl

A cytological study of Nematospora coryli pegl

Further Cytological Studies in the Genus Penicillium Link

DNA Content of Germinating Spores, Individual Hyphal Cells and Resting Structure Cells of Verticillium spp. Measured by Microdensitometry

Contact Info

Product

Resources

About