Karyogamy and Meiosis in the Rust Coleosporium Vernoniae

Olive, Lindsay S.

doi:10.1002/j.1537-2197.1949.tb05231.x

Cited by 39 publications

(4 citation statements)

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“…1974;Williams and Hartley 1971). Karyotypic studies in other rust fungi have also relied on light microscopic analysis of nonpachytene nuclei and have yielded haploid chromosomc numbers similar to those reported by McGinnis (1953) for P. grmzinis (Allen 1933;McGinnis 1954;Olive 1949;Singh 1972;Valkoun and Bartos 1974;Wright and Leonard 1978). Sansome (1959) was the first to derive a karyotype for a rust fungus by counting pachytene bivalents; a haploid chromosome number of ir = 20-22 was determined for Puccirzia krr~ussiaiza by light microscopy of pachytene bivalents released from crushed teliospores and stained with aceto-carmine.…”

Section: Earlier Rust Jiiizgus Katyotypesmentioning

confidence: 76%

An ultrastructural pachytene karyotype for Puccinia graminis f.sp. tritici

Boehm¹,

Wenstrom²,

McLaughlin³

et al. 1992

Can. J. Bot.

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The karyotype for Puccinia graminis f.sp. tritici was determined from reconstructions of electron micrographs of serially sectioned pachytene nuclei. Epifluorescence microscopy was used to select DAPI-stained, pachytene nuclei in teliospore protoplasts from which walls were mechanically removed. Selection increased the probability that pachytene nuclei could be found in the absence of morphological markers. Six pachytene nuclei were reconstructed from four geographically disparate North American isolates. One nucleus was used to obtain a computer-enhanced, three-dimensional reconstruction that could be rendered as rotatable colorized stereo pairs. A karyotype of n = 18 was determined for all six nuclei. The 18 bivalents varied only slightly in size, each ranging from 3.0 to 8.8% of the total length of the genome. Total genomic lengths also proved highly comparable among isolates. Centromeres were not found, precluding use of the centromeric index as an aid in karyotyping. Only the nucleolus-associated bivalent could be cross-correlated among the six reconstructed nuclei. Heterogeneity in length among, but not within, isolates was observed for this bivalent, suggesting that chromosome length may be polymorphic in P. graminis f.sp. tritici, despite a constant total number of chromosomes. Key words: chromosomes, heterobasidiomycete, synaptonemal complex, Uredinales.

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Section: Earlier Rust Jiiizgus Katyotypesmentioning

confidence: 76%

An ultrastructural pachytene karyotype for Puccinia graminis f.sp. tritici

Boehm¹,

Wenstrom²,

McLaughlin³

et al. 1992

Can. J. Bot.

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“…In this idea, re-orientation including re-arrangement of homologous chromosomes takes place just after syngamy regardless whether it is followed with mitosis in diploid organisms, or zygotic meiosis in haploid organisms. In zygotic meiosis of fungi, chromosome pairings essentially occur between contracted chromosomes (McClintock 1945;Olive 1949;Singleton 1953, etc. ) instead between highly elongated ones.…”

Section: Discussionmentioning

confidence: 99%

“…pairing of homologous regions on translocation chromosomes was observed in Drosophila (Hinton 1946), and the somatic chromosome number was not of 2 n but of n in interhpase of Daphne (Hiraoka 1958). And the initiation of synapsis was observed in several fungi to occur between contracted chromosomes instead between elongated ones, mainly associated with the phenomenon of bouquet orientation prior to zygotic meiosis (McClintock 1945;Olive 1949;Singleton 1953;El-Ani 1956), and the presence of bouquet stage was reported in ordinary meiosis too (Hughes-Schrader 1943;Smith 1956).…”

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confidence: 99%

“…For example, somatic recombination requires somatic pairing at the exactly same loci of homologous chromosomes (Pontecorvo 1956, etc. in fungi ;Stern 1936, in Drosophila), and autoradiographic data of Taylor (1958), half-chromatid breaks by radiation (Oestergren andWakoning 1954, andSwanson 1957), half chromatid mutation in Drosophila (Muller, Carlson, and Schalet 1961) and aberrant gene segregations with 5:3 ratio in Sordaria (Olive 1959;Kitani, Olive, and El-Ani 1962) requir the doubleness of so called monad chromosome.…”

mentioning

confidence: 99%

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