Karymorphological and molecular studies on seven species in Polygonoideae (Polygonaceae) in Egypt

Abstract

ABSTRACT. Seven species in four genera of the Polygonoideae (Polygonaceae) in Egypt were subjected to karyomorphological and molecular studies in order to identify their chromosomal characteristics and investigate their phylogenetical relationships by the conventional staining method and the 5S rDNA PCR. Seed germination after treatment with low temperature stratifi cation and acidifi cation by concentrated H 2 SO 4 was studied. Three rates of germination were obtained in response to the cold stratifi cation and acidifi cation: 1) High in Polygonum equisetiforme, Persicaria lanigera, Pe. lapathifolia and Pe. salicifolia; 2) low in Rumex dentatus; 3) no effect in R. pictus and Emex spinosa. Variation in the chromosome complements number, length and structure were detected for Po. equisetiforme (2n=58; new count); Pe. lanigera (2n=40; new count); Pe. lapathifolia (2n=22); Pe. salicifolia (2n=60); Emex spinosa (2n=18; a new count); Rumex dentatus (2n=40); and R. pictus (2n=18; a new count). Eighteen polymorphic bands of 5S rDNA were used to determine the similarities among the taxa with the similarity coeffi cient ranging between 0.2 and 0.67.

Species differentiation of two cultivar species in <i>Fagopyrum</i> (Polygonaceae)

“…The PCR product was purified using the Qiagen kit protocol (Qiagen, Germany), and the results were verified using 1% agarose gel. The 5S rDNA primers have the same sequences as the ones used in El-Twab et al (2012), and the 45S rDNA primers were designed with NCBI blast based on the information by Yasui and Ohnishi (1998) and the sequence of ITS1-5.8S-ITS2 region registered in the DDBJ/EMBL/Gene Bank database. FISH probes were labeled using the nick-translation method.…”