Karasal bitkilerde DNA barkodlama: Bazı DNA barkod bölgelerinin incelenmesi

Abstract

DNA Barcoding in Terrestrial Plants: Investigation of Some DNA Barcode RegionsAbstract: DNA barcoding is a technique that has been widely used to identify species and discover phylogenetic utilities of species for last 30 years. The main goal of this technique is to discriminate organisms at species level by short DNA fragments originated from nucleus or plastids. Although nucleus originated barcoding regions provide more data for species identification rather than plastid regions, none of the single-locus regions is adequate for universality and provides enough data for comparing plant groups. There is no barcoding region available to be used for all plant groups. Therefore, combining more than one barcoding regions could improve resolution.Using short DNA fragments as barcoding region is achieved to top saturation and new techniques are being studied by researchers. The whole chloroplast genome has enormous data and this data could provide enough specificity for universal barcoding for land plants.

Analysis of Various DNA Barcodes on the Turkish Protected Designation of Origin Apricot “Iğdır Kayısısı” (Prunus armeniaca cv. Şalak)

“…Each DNA barcoding region has different characteristics due to its origin. The Internal Transcribed Spacer (ITS) region of the nuclear ribosomal cistron is one of the most used DNA barcoding regions for the plants for 25 years (Hürkan, 2017), due to its universality, ease of amplification, good polymorphism rate, and ideal barcode length (~630 bp) (White et al, 1990). The region combines both coding and non-coding sequences which empower the resolution power of the complicated plant groups (Kress et al, 2005).…”

The importance in DNA barcoding of the regions which is covering rRNA genes and its sequences in the genus Quercus L.

“…The cytochrome c oxidase-1 (CO1) gene from the mitochondria has enough nucleotide differentiation rates to identify many groups of animals and is routinely used to identify new species as an universal barcode (Hebert et al, 2003(Hebert et al, , 2004Greenstone et al, 2005;Ward et al, 2005;Smith et al, 2006;Piredda et al, 2011;Hürkan, 2017) but this rate is relatively low and unsuitable in plants (Chase et al, 2005;Kress et al, 2005;Fazekas et al, 2008;Hollingsworth et al, 2009). Therefore alternative barcode regions should be screened that can be universally successful in all species, however such a barcode region has not been found yet (Chase and Fay, 2009;Hollingsworth et al, 2009).…”