KARAKTERISTIK FISIKO-KIMIA DAN AKTIVITAS ANTIOKSIDAN KITOSAN YANG DIPRODUKSI DARI SISIK IKAN KAKATUA (Scarus sp.)

Abstract

The aims of this study was to determine the physicochemical characteristics and to know the antioxidant activity of chitosan parrot fish scales (Scarus sp.). The test parameters used in this study include color, particle size, moisture content, ash content, nitrogen content, degree of deacetylation, viscosity, molecular weight and antioxidant activity. Chitosan is synthesized from the fish scales through the demineralization, deproteination, and deascetylation stage. Viscosity was measured using an viscometer Ostwald, degree of deacetylation e using an Spectrophotometer IR, and antioxidant activity was tested using the DPPH Method. The results of the analysis showed that chitosan scales of parrot fish (Scarus sp.) Produced in this study had physical properties namely white and powder size, and had a moisture content of 0.37%, ash content of 0.48%, nitrogen content of 0.38% , deacetylation degree of 90.23%, viscosity 1.19 cP and molecular weight 24.547 kDa. Chitosan antioxidant activity test has an IC50 value of 3004.66 ppm which is classified as having weak antioxidant activity because it is more than 200 ppm. Based on these results, the chitosan produced in this study can be said to have good quality, but chitosan scales of parrot fish (Scarus sp.) Have potential as antioxidants even though but are relatively weak.