KARAKTERISTIK FERMENTATIF MEDIUM deMann Rogosa Sharpe (MRS) ANTOSIANIN BERAS KETAN HITAM (Oryza sativa var. glutinosa) MENGGUNAKAN Pediococcus pentosaceus N11.16

Abstract

Anthocyanin was a bioactive compound which has an anti-infl ammatory effect, anticancer, antimutagen, antioxidant, anticholesterol, and also acts as therapheutic agent for artherioschlerosis. Initial step for anthocyanin metabolism was hydrolyzed to anthocyanidin (aglikon) by β-D-glucosidase. Pediococcus pentosaceus N11.16 is known to be one of bacteria which has a capability to hydrolyze black glutinous rice anthocyanin extract. The aims of this research were to study the fermentative characteristics of MRS containing black glutinous rice anthocyanin medium (MRSm+Anthocyanin) using lactic acid bacteria P. pentosaceus N11.16. The results showed that P. pentosaceus N11.16 could grew well in this medium. Total acid producing bacteria increased untill 2 log cycle with antioxidant activity (%RSA) 75% and the Fe 3+ ion reducing capacity 36.75%. Phenolic compound in the MRSm+ anthocyanin was 584.05 mg asam galat/100 mL after being fermented for 16 hours. Phenolic compound in MRSm+anthocyanin medium were higher than MRSm or control medium (MRS). Beta glucosidase activities of the bacterial cell tend to be higher in theMRS anthocyanin medium than MRS without sugar and control medium.Keywords: Anthocyanin, black glutinous rice, lactic acid bacteria, antioxidant ABSTRAKLangkah awal dalam metabolisme antosianin, komponen bioaktif utama yang terdapat dalam beras ketan hitam, adalah hidrolisis menjadi antosianidin (aglikon) dengan memanfaatkan enzim β,D-glukosidase. Pediococcus pentosaceus N11.16 merupakan salah satu bakteri yang telah diisolasi dan teruji kemampuannya untuk menghidrolisis antosianinberas ketan hitam. Penelitian ini bertujuan untuk mempelajari karakteristik fermentatif medium fermentasi yang mengandung antosianin beras ketan hitam menggunakan bakteri asam laktat P. pentosaceus N11.16. Penelitian dilakukan dengan cara menguji kemampuan tumbuh isolat dalam medium MRS yang dimodifi kasi (MRSm) dengan penambahan antosianin beras ketan hitam. Hasil menunjukkan bahwa bakteri P. pentosaceus N11.16 mampu tumbuhdengan baik dalam medium MRS yang mengandung antosianin beras ketan hitam. Kenaikan mencapai 2 log cycle dengan aktivitas antioksidan (%RSA) mencapai 75% dan kemampuan menangkap logam mencapai 36,75%. Total fenol medium MRSm+antosianin mencapai 584,05 mg asam galat/100 mL medium setelah 16 jam fermentasi. Totalfenol medium MRSm+antosianin cenderung lebih tinggi daripada MRSm atau kontrol (MRS). Aktivitas enzim β glukosidase sel bakteri cenderung lebih tinggi pada medium MRSm+antosianin daripada MRSm atau kontrol (MRS).Kata kunci: Antosianin, beras ketan hitam, bakteri asam laktat, antioksidan

The Potential of Glutinous Rice Tape Added with Lactobacillus plantarum Dad-13 and Saccharomyces boulardii as a Probiotic Food

“…The β-glucosidase enzyme is an enzyme that is trapped in the cell wall and has extracellular activity. It is produced by the bacteria L. plantarum Mut 7 (Suhartatik et al, 2013), Pediococcus pentosaceus N11.16 (Suhartatik et al, 2014), a subspecies of Lactobacillus plantarum, Lactobacillus casei LC-01 and L. casei IFPL7190 (Ávila et al, 2009). Table 2 shows that the addition of the inoculum did not significantly affect the anthocyanin levels in white glutinous rice tape.…”

Evaluation of jamu kunyit asam (Curcuma domestica Val. - Tamarindus indica L.) as probiotic carrier of Lactobacillus plantarum BP102

“…Since the implementation was carried out at different times, differences in the test materials used could affect the results of antioxidant activity. However, Suhartatik et al (2014) showed that the culture of L. plantarum BP102 in MRS broth medium had an antioxidant activity of about 65%. This antioxidant activity occurred due to the presence of phenol compounds in the culture, which was possible from the components of MRS broth which had a phenolic structure, such as amino acids in the beef extract.…”

Pengaruh Pemberian Beras Ketan Hitam (Oryza sativa L. Var glutinosa) Terhadap Perubahan Kadar Gula Darah Tikus Wistar Yang Diinduksi Streptozotocin-NA

“…Akibatnya, sekresi insulin menurun dan menyebabkan gula darah tinggi. 10 Untuk mencegah penghancuran seluruh sel pankreas, tikus juga disuntik dengan NA, yang dapat melindungi sebagian sel pankreas. 12 Terdapat perbedaan yang signifikan pada kadar GDP kelompok negatif (K-) dan kelompok positif (K+) penyebab rendahnya kadar GDP pada kelompok (K-) dikarenakan pada kelompoknya tidak diinjeksi dengan STZ dan NA.…”