Kappa light chain mRNA bearing plasma cells are predominant in periodontitis lesions

Takahashi, Keiso; Moughal, N. A.; Mooney, John J.; Kinane, D. F.

doi:10.1111/j.1600-0765.1996.tb00490.x

Cited by 10 publications

(15 citation statements)

References 18 publications

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“…Plasma cells and epithelium, especially basal layers, were usually strongly stained (data not shown). Further, we have shown strong signals for immunoglobulin light chain mRNA in plasma cells by non-isotopic ISH [22].…”

Section: Discussionmentioning

confidence: 57%

“…It has been reported that the in situ hybridization method for the detection of immunoglobulin light chain mRNA-positive cells gives different results from those obtained by immunohistochemistry [15,20,21]. The differences between the two techniques have been confirmed by Sandholm and Saxen and in our laboratory from studies of inflamed gingiva [11,22].…”

Section: Introductionmentioning

confidence: 60%

See 1 more Smart Citation

IgG and IgA subclass mRNA-bearing plasma cells in periodontitis gingival tissue and immunoglobulin levels in the gingival crevicular fluid

Takahashi

Mooney²,

Frandsen

et al. 1997

Clinical and Experimental Immunology

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SUMMARYThe humoral immune response, especially the production of IgG and IgA, is considered to have a protective role in the pathogenesis of periodontal disease, but the precise mechanisms are still unknown. In order to determine local IgG and IgA production, we investigated the presence of human IgG and IgA subclass mRNA-bearing plasma cells within periodontal tissue by in situ hybridization using digoxigenin-labelled oligonucleotide probes in 24 gingival biopsy samples (pocket depth 5 5 mm) which were obtained from eight patients with adult periodontitis. Furthermore, we examined IgG and IgA subclass proteins and digested IgA1 Fab portions in the gingival crevicular fluid (GCF), corresponding to the sites from which the tissues were taken, by ELISA. IgG and IgA subclass mRNA-expressing cells were detected in all serial formalin-fixed/paraffinembedded gingival tissue sections sampled. Plasma cells showed strong cytoplasmic staining with a high contrast and a good retention of morphology with these probes. IgG1 mRNA-expressing cells were predominant (mean 63%) and IgG2 mRNA-expressing cells were present at around 23% of total IgG plasma cells, while IgG3 and IgG4 mRNA-expressing cells were present to a much lesser extent (3% and 10%, respectively). Similar proportions of IgG subclass proteins in GCF were detected, which were also consistent with 'normal' serum levels. In terms of IgA subclass, IgA1 mRNA-positive cells were predominant (mean 65 . 1%, P

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Section: Discussionmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 60%

IgG and IgA subclass mRNA-bearing plasma cells in periodontitis gingival tissue and immunoglobulin levels in the gingival crevicular fluid

Takahashi

Mooney²,

Frandsen

et al. 1997

Clinical and Experimental Immunology

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“…This may be due to hyperactivity influenced by an imbalance in regulatory T-lymphocytes which dominated the periodontal lesion. [3233] Takahashi et al, [13] reported that activated B-cell or plasma cell produced immunoglobulins, with the light chain κ of the immunoglobulin being predominant in periodontitis lesions. Hence, κ light chain was chosen as a marker for activated B-cell or plasma cell.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that antibodies produced against periodontal pathogens have a predominance of κ light chains. [13] Hence, κ light chain was chosen as a marker for activated B-cell or plasma cell.…”

Section: Methodsmentioning

confidence: 99%

Lymphocyte subpopulation in healthy and diseased gingival tissue

Amunulla

Venkatesan

Hemalatha

et al. 2008

J Indian Soc Periodontol

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In this study, infiltrating lymphocytes subpopulation in gingival sections of healthy, inflamed, and periodontitis lesions was investigated. A set of cluster of differentiation (CD) antigen specific monoclonal/polyclonal antibodies to detect different cell types within the tissues was used. These included anti-CD3 (pan T-cell), anti-CD45RO (memory T-cell), anti-CD20 (B-cell), and kappa light chain (plasma cells). Biopsies of gingival tissue were obtained from 17 patients who had clinically healthy gingiva, from 18 patients with gingivitis, and 17 patients with periodontitis.A significantly greater proportion of T-cells (P < 0.00) was observed in healthy gingival and gingivitis tissue samples compared to periodontitis tissue samples. In addition, a greater proportion of B-cells was observed in periodontitis lesions than in the gingival lesions (P < 0.00). The memory T-cells and the kappa light-chain plasma cells were present in both healthy and diseased tissues, suggestive of previous activation by periodontal pathogenic microorganisms.In conclusion, these differences in the relative proportions of B- and T-cells may reflect a difference in the immunopathology of periodontitis and gingivitis lesions.

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“…The order of immune cell infiltration into the gingiva during PD etiology therefore differs from the order identified in adipose tissue from the diet-induced obese (DIO) mouse model of insulin resistance and T2D (see above). The percentage of B cells in chronic PD lesions positively correlates with disease severity [50, 52–54], suggesting B cells promote rather than initiate PD. The B cells in human PD lesions have a transitional or activated phenotype, as indicated by surface expression of the B cell activation marker CD5 [55–59].…”

Section: Roles For B Cells In Inflammatory Diseasementioning

confidence: 99%

B cells as under-appreciated mediators of non-auto-immune inflammatory disease

Nikolajczyk

2010

Cytokine

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B lymphocytes play roles in many autoimmune diseases characterized by unresolved inflammation, and B cell ablation is proving to be a relatively safe, effective treatment for such diseases. B cells function, in part, as important sources of regulatory cytokines in autoimmune disease, but B cell cytokines also play roles in other non-autoimmune inflammatory diseases. B cell ablation may therefore benefit inflammatory disease patients in addition to its demonstrated efficacy in autoimmune disease. Current ablation drugs clear both pro- and anti-inflammatory B cell subsets, which may unexpectedly exacerbate some pathologies. This possibility argues that a more thorough understanding of B cell function in human inflammatory disease is required to safely harness the clinical promise of B cell ablation. Type 2 diabetes (T2D) and periodontal disease (PD) are two inflammatory diseases characterized by little autoimmunity. These diseases are linked by coincident presentation and alterations in Toll-like receptor (TLR)-dependent B cell cytokine production, which may identify B cell ablation as a new therapy for co-affected individuals. Further analysis of the role B cells and B cell cytokines play in T2D, PD and other inflammatory diseases is required to justify testing B cell depletion therapies on a broader range of patients.

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Kappa light chain mRNA bearing plasma cells are predominant in periodontitis lesions

Cited by 10 publications

References 18 publications

IgG and IgA subclass mRNA-bearing plasma cells in periodontitis gingival tissue and immunoglobulin levels in the gingival crevicular fluid

IgG and IgA subclass mRNA-bearing plasma cells in periodontitis gingival tissue and immunoglobulin levels in the gingival crevicular fluid

Lymphocyte subpopulation in healthy and diseased gingival tissue

B cells as under-appreciated mediators of non-auto-immune inflammatory disease

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