Kaposi’s sarcoma herpesvirus load in biopsies of cutaneous and oral Kaposi’s sarcoma lesions

Pak, Fatemeh; Mwakigonja, Amos; Kokhaei, Parviz; Hosseinzadeh, Nahid; Pyakurel, Pawan; Kaaya, Ephata; Bogdanović, Gordana; Selivanova, Galina; Biberfeld, Peter

doi:10.1016/j.ejca.2007.05.023

Cited by 26 publications

(30 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some studies reported an increase in the KSH/HHV8 DNA load of patients with clinical KS [21,28,29]. In contrast, other studies showed a decrease in the HHV8 viral load in sera from patients with late stages of KS, indicating the probability of suppression of HHV8 release and/or immunosegregation of virus during these later stages [26,[30][31][32]. Another study showed that measurement of the HHV8 load [33].…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

LANA-1, Bcl-2, Mcl-1 and HIF-1α protein expression in HIV-associated Kaposi sarcoma

et al. 2009

View full text Add to dashboard Cite

Human herpesvirus 8 (HHV8) is necessary for Kaposi sarcoma (KS) to develop, but whether the tissue viral load is a marker of KS progression is still unclear. Little is known about the level of expression of apoptosis-regulating proteins and of hypoxia-inducible factors (HIFs) in KS tumour cells relative to HHV8 expression. We therefore investigated the expression of the latency-associated nuclear antigen (LANA-1) of HHV8, Bcl-2, Mcl-1, Bax, Bcl-xL, caspase 3 and HIF-1alphain KS tissue specimens at different stages of the disease. The expression of these proteins was evaluated immunohistochemically using tissue microarrays (TMAs) in tissue specimens from 245 HIV-positive patients at different stages of the disease. Both LANA-1 and HIF-1alpha were expressed in KS biopsies taken at different stages, but their level increased throughout tumour progression. Additionally, the levels of Bcl-2 and Mcl-1 were higher in visceral KS lesions compared to levels observed in cutaneous and mucosal KS. This study demonstrates that late tumour stages of KS in tissues from HIV-positive patients are associated with high levels of LANA-1, HIF-1alpha and of the anti-apoptotic proteins, Bcl-2 and Mcl-1. Finally, the expression of these proteins can be potentially used as a tissue biomarker in defining patients with a higher risk of disease progression.

show abstract

Section: Discussionmentioning

confidence: 94%

“…The natural history and pathogenesis of HHV8 infections in immunosuppressed patients has been extensively studied by different groups [21,[26][27][28][29][30]. The identification of KS patients with poor survival is essential in determining the treatment strategy.…”

Section: Discussionmentioning

confidence: 99%

LANA-1, Bcl-2, Mcl-1 and HIF-1α protein expression in HIV-associated Kaposi sarcoma

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Usually superficial radiation beams, such as electron beams (400 cGy once weekly for 6-8 consecutive weeks), are used. Although systemic chemotherapy is often effective in the treatment of classic KS, it is not generally recommended in AIDS-related KS because it leads to further immunosuppression, which can affect survival [147]. Current systemic therapy for KS includes liposomal anthracyclines (doxorubicin and daunorubicin) and paclitaxel.…”

Section: Kaposi Sarcomamentioning

confidence: 98%

Sarcomas of the head and neck in adult patients: current concepts and future perspectives

Rapidis

2008

Expert Review of Anticancer Therapy

View full text Add to dashboard Cite

Sarcomas comprise a heterogeneous and biologically diverse group of malignant neoplasms having as a common denominator their origin from mesenchymal cells. Head and neck sarcomas account for 4 to less than 20% of total body sarcomas depending on the criteria, such as age of patients (pediatric vs adult population), type of sarcomas (soft-tissue vs bony sarcomas) and site of location. Although head and neck sarcomas occur infrequently in adults, in the pediatric population one in three sarcomas will occur in the head and neck region. Most head and neck sarcomas are of the soft-tissue type, with only 20% being of bony or cartilaginous origin. Sarcomas display a diverse array of histologies and a wide spectrum of clinical behavior, ranging from relatively slow growing lesions to aggressive locally and regionally destructive tumors with the potential for systemic metastases. Osteosarcomas, rhabdomyosarcomas, pleomorphic sarcomas (malignant fibrous histiocytomas), fibrosarcomas and angiosarcomas are among the most common histologic types of sarcoma found in the head and neck. Surgery has been the primary therapeutic approach for the management of head and neck sarcomas. Survival rates for head and neck sarcomas suggest worse outcomes than for their extremity counterparts. Lymph node metastasis only occurs in 3-10% of sarcomas of the head and neck. An improvement in local disease control has recently been suggested with the combined use of surgery and radiotherapy. Conflicting results have been reported on the benefit from the use of chemotherapy as an adjuvant or neoadjuvant regimen, especially for high-grade sarcomas in long-term survival or local disease control. Encouraging results have recently been reported with the use of molecular targeted therapies with tyrosine kinase inhibitors and antiangiogenetic agents.

show abstract

“…Gene expression investigations in human diseases provide useful diagnostic and prognostic approaches for many disorders (1)(2)(3). Tissue specimens can be kept for a long time after fixing in formalin and embedding in paraffin (FFPE).…”

Section: Introductionmentioning

confidence: 99%

An Infant With Non-Ketotic Hyperglycemia: A Case Report

Danaei

Nooripour

2016

Middle East J Rehabil Health

View full text Add to dashboard Cite

Background: RNA extraction from Formalin Fixed Paraffin Embedded tissue (FFPE) provides valuable information. The main obstacle for pure RNA extraction from FFPE specimens is RNA degradation over time and low yield of RNA due to chemical processing. In the present study, RNA extraction from FFPE specimens were optimized for storage time, proteinase K concentration, and tissue size hemogenation. Methods: To evaluate the effect of storage time on RNA extraction yield, total RNA from 78 FFPE breast tissue specimens (1 -3 years n = 52, and less than one year, n = 26) were extracted by High Pure Paraffin Kit (Roche). The effect of 2 different proteinase K on RNA

show abstract

Kaposi’s sarcoma herpesvirus load in biopsies of cutaneous and oral Kaposi’s sarcoma lesions

Cited by 26 publications

References 37 publications

LANA-1, Bcl-2, Mcl-1 and HIF-1α protein expression in HIV-associated Kaposi sarcoma

LANA-1, Bcl-2, Mcl-1 and HIF-1α protein expression in HIV-associated Kaposi sarcoma

Sarcomas of the head and neck in adult patients: current concepts and future perspectives

An Infant With Non-Ketotic Hyperglycemia: A Case Report

Contact Info

Product

Resources

About