Kangiella taiwanensis sp. nov. and Kangiella marina sp. nov., marine bacteria isolated from shallow coastal water

Abstract

Two Gram-negative, heterotrophic, aerobic, marine bacteria, designated strains KT1T and KM1T, were isolated from seawater samples collected from the shallow coastal regions of northern Taiwan. Cells grown in broth cultures were non-flagellated rods. NaCl was required for growth. Optimal growth occurred with 2–5 % NaCl, at 25–30 °C and at pH 8. They grew aerobically and were not capable of anaerobic growth by fermenting d-glucose or other carbohydrates. Q-8 was the only isoprenoid quinone. The major polar lipid detected in strain KT1T was phosphatidylmonomethylethanolamine, whereas those detected in KM1T were phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine and an unidentified phospholipid. Cellular fatty acids were nearly all iso-branched, with iso-C15 : 0 as the most abundant component (54.6–57.2 % of the total). Strains KT1T and KM1T had DNA G+C contents of 43.9 and 46.3 mol%, respectively. The two strains shared 98.1 % 16S rRNA gene sequence similarity; levels of similarity with the type strains of species of the genus Kangiella were 95.6–98.4 %. Data from the present taxonomic study conducted using a polyphasic approach revealed that the isolates could be classified as representatives of two novel species of the genus Kangiella , for which the names Kangiella taiwanensis sp. nov. (type strain KT1T = BCRC 80330T = JCM 17727T) and Kangiella marina sp. nov. (type strain KM1T = BCRC 80329T = JCM 17728T) are proposed.

Lignocellulose-responsive bacteria in a southern California salt marsh identified by stable isotope probing

“…Cultured members of this group have not previously been shown to degrade complex carbon (Yoon et al, 2004; Jean et al, 2012) suggesting possible new roles for Kangiella either directly using lignocellulose or indirectly using lignocellulose-derived degradation by-products in the HBW marsh. Alternatively, we cannot rule out the possibility that during the 30-day incubation period, cross feeding among bacterial groups occurred in this microcosm, which could also result in labeling of unexpected lineages such as Kangiella .…”

Kangiella profundi sp. nov., isolated from deep-sea sediment

“…The genome sequence of the type strain of the type species Kangiella koreensis was determined as part of the Genomic Encyclopedia of Bacteria and Archaea project (Han et al, 2009). At the time of writing, the genus Kangiella consists of eight species with validly published names, and the type strains of all species were isolated from samples collected in shallow water marine environments, including tidal flat zones, coastal seawater and sediments, and marine sponges (Yoon et al, 2004(Yoon et al, , 2012Romanenko et al, 2010;Ahn et al, 2011;Jean et al, 2012;Lee et al, 2013). The genera Kangiella and Alcanivorax are the only sibling genera in the family Alcanivoraceae.…”

“…Differential phenotypic characteristics of strain FT102 T and the type strains of eight recognized species of the genus Kangiella Strains: 1, FT102 T (data from this study); 2, K. koreensis JCM 12317 T (this study); 3, K. aquimarina JCM 12318 T (this study); 4, K. japonica JCM 16211 T (Romanenko et al, 2010); 5. K. spongicola DSM 23219 T (Ahn et al, 2011); 6, K. taiwanensis JCM 17727 T (Jean et al, 2012); 7, K. marina JCM 17728 T (Jean et al, 2012); 8, K. geojedonensis KCTC 23420 T (Yoon et al, 2012); 9, K. sediminilitoris KCTC 23892 T (Lee et al, 2013 Yoon et al (2004). (Lee et al, 2013).…”

Aliikangiella marina gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122, and proposal of Kangiellaceae fam. nov. in the order Oceanospirillales

“…Recently, the genus Kangiella was assigned to this family based on molecular analyses of 16S rRNA gene sequences (Silveira & Thompson, 2014). However, phylogenetic results of the latest The All-Species Living Tree (Release LTPs119, Munoz et al , 2011) and species identification in the genera Kangiella and Pleionea do not support this assignment (Yoon et al , 2004, 2012; Romanenko et al , 2010; Ahn et al , 2011; Jean et al , 2012; Fagervold et al , 2013; Lee et al , 2013; Kim et al , 2015; Xu et al , 2015), so the exact taxonomic standings of these Kangiella -related organisms are uncertain (Fagervold et al , 2013). In this study, strain GYP-15 T , phylogenetically close to the genera Kangiella and Pleionea , was isolated from the culture broth of a marine microalga, Picochloruma sp.…”