Kanamycin is effective for selecting transformed peas

Grant, J. E.; Cooper, Pauline A.; Gilpin, Brent; Hoglund, Sonja J.; Reader, J.K.; Pither-Joyce, Meeghan; Timmerman-Vaughan, Gail M.

doi:10.1016/s0168-9452(98)00184-8

Cited by 38 publications

(24 citation statements)

References 11 publications

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“…This improvement over cocultivation with strain AGL 1, which is routinely used for pea transformation (Grant et al 1995(Grant et al , 1998Schroeder et al 1993;Morton et al 2000), was at least fourfold for all except one experiment in which there was no improvement. The improvement was consistent over environmental and physiological growth conditions of pea mother plants.…”

Section: Discussionmentioning

confidence: 95%

“…For each experiment, the peas were picked from the field at the same time, and cocultivation with Agrobacterium tumefaciens strains AGL 1 or KYRT1 was carried out on the same day to standardize conditions as much as possible. Preparation of the explants, cocultivation and culture conditions were as reported in Grant et al (1995;1998). The two binary vectors used, pTGUS and pMG, were based on pGA643 (An et al 1988).…”

Section: Plant Materials and Pea Transformationmentioning

confidence: 99%

“…Torisky et al (1997) reported the development of a disarmed strain of A. tumefaciens Chry5 called KYRT1 that was equally or more effective than strains EHA105 or GV3850 at delivering DNA into soybean cotyledonary node explants. In the investigation reported here, we compared the efficacy of KYRT1 with that of strain AGL 1 using our regular transformation method (Grant et al 1995(Grant et al , 1998 over three summers and with two cultivars, Bolero and Lincoln, and one process pea breeding line.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Agrobacterium tumefaciens strain on the production of transgenic peas ( Pisum sativum L.)

et al. 2003

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We compared the efficiency of two Agrobacterium tumefaciens strains, AGL 1 and KYRT1, for producing transgenic pea plants. KYRT1 is a disarmed strain of Chry5 that has been shown to be highly tumourigenic on soybean. The efficacies of the strains were compared using cotyledon explants from three pea genotypes and two plasmids. The peas were sourced from field-grown plants over three Southern Hemisphere summer seasons. Overall, KYRT1 was found to be on average threefold more efficient than AGL 1 for producing transgenic plants. We suggest that KYRT1 is sensitive to cocultivation temperature as the expected increase in efficiency was not achieved at high laboratory temperatures.

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Section: Discussionmentioning

confidence: 95%

Section: Plant Materials and Pea Transformationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of Agrobacterium tumefaciens strain on the production of transgenic peas ( Pisum sativum L.)

et al. 2003

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“…Puonti-Kaerlas (1992), De Kathen and Jacobsen (1990) as well as Schroeder et al (1993) found kanamycin less effective for selection than hygromycin and phosphinotricin. On the other hand, Grant et al (1998) deemed kanamycin in higher concentrations and in combination with successive selection steps as effective. Similar results were published by Morton et al (2000).…”

Section: Agrobacterium-mediated Transformationmentioning

confidence: 99%

“…Thenceforth, a number of protocols have been reported for Agrobacteriummediated gene transfer in pea (Bean et al 1997;Grant et al 1998;NadolskaOrczyk and Orczyk 2000;Pniewski and Kapusta 2005;Polowick et al 2000;Schroeder et al 1994;, as listed in Table 10. In the procedure described by Polowick et al (2000), segments of the embryogenic axis are used.…”

Section: Peamentioning

confidence: 99%

Gene Transfer in Legumes

Atif¹,

Patat-Ochatt²,

Švábová

et al. 2012

Progress in Botany

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Pea

McPhee¹

2008

Compendium of Transgenic Crop Plants

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Development of gene technology methods for plants has allowed novel genes to be introduced, where natural variation is lacking, irrespective of hybridization barriers. Pea, an important agricultural crop worldwide, lacks certain genes for disease resistance and would benefit from introduction of novel resistance traits. In addition to its role in agricultural production, pea plays a vital dietary role in developing countries and could serve as a valuable vehicle to provide enhanced nutrition to malnourished populations. Gene technology in pea was first studied in the late 1980s and viable protocols for gene introduction and selection of transformed plants were established in the early 1990s. Although other methods have been tested, Agrobacterium tumefaciens ‐mediated methods for gene introduction are preferred. Several examples of successful transformation have been reported and the introduction of the α‐amylase gene from common bean for resistance to Bruchus pisorum , the pea seed weevil, has potential to overcome the adverse effects of this agronomically important pest. Consideration of regulatory hurdles and intellectual property rights are crucial to the successful implementation of gene technology and has hindered the use of Bruchid‐resistant peas. Application of gene technology has its greatest potential in basic studies of gene function. Gene complementation experiments allow the function of candidate genes to be verified and characterized. Overall, gene technology is a very powerful tool and is expected to have a significant impact on crop improvement and genomic evaluation of all crop plants.

show abstract

Kanamycin is effective for selecting transformed peas

Cited by 38 publications

References 11 publications

Influence of Agrobacterium tumefaciens strain on the production of transgenic peas ( Pisum sativum L.)

Influence of Agrobacterium tumefaciens strain on the production of transgenic peas ( Pisum sativum L.)

Gene Transfer in Legumes

Pea

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